PMID- 37816718
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231123
IS  - 2058-7716 (Print)
IS  - 2058-7716 (Electronic)
IS  - 2058-7716 (Linking)
VI  - 9
IP  - 1
DP  - 2023 Oct 10
TI  - IGF2BP2 acts as a m(6)A modification regulator in laryngeal squamous cell 
      carcinoma through facilitating CDK6 mRNA stabilization.
PG  - 371
LID - 10.1038/s41420-023-01669-7 [doi]
LID - 371
AB  - Laryngeal squamous cell carcinoma (LSCC) is one of the most commonly seen cancers 
      in the head and neck region with increasing morbidity and mortality globally. 
      N6-methyladenosine (m(6)A) modification plays a critical role in the 
      carcinogenesis of LSCC. In this study, two datasets from online database were 
      analyzed for differentially expressed genes (DEGs) between LSCC and normal 
      samples. Furthermore, we carried out a series of experiments, including 
      hematoxylin & eosin staining, immunohistochemical (IHC) staining, CCK-8, colony 
      formation, transwell, flow cytometry, xenograft tumor model assays, actinomycin D 
      assay, cycloheximide (CHX) assay, methylated m(6)A RNA immunoprecipitation 
      (Me-RIP), RNA immunoprecipitation (RIP) assay, to verify the relevant findings in 
      vivo and in vitro. Insulin like growth factor 2 mRNA binding protein 2 (IGF2BP2) 
      was identified as an up-regulated m(6)A regulator in LSCC samples. Lower IGF2BP2 
      expression was linked to higher survival probability in LSCC and other head and 
      neck squamous cell carcinoma patients. In LSCC cells, IGF2BP2 knockdown 
      attenuated cancer cell aggressiveness, possibly through modulating cell cycle 
      arrest. In the xenograft tumor model derived from IGF2BP2 knocked-down LSCC 
      cells, IGF2BP2 knockdown inhibited tumor growth. IGF2BP2 up-regulated CDK6 
      expression through facilitating the stability of CDK6 mRNA and protein. CDK6 
      knockdown caused no changes in IGF2BP2 expression, but partially eliminated the 
      promotive effects of IGF2BP2 overexpression on LSCC cells' aggressiveness. 
      Overexpressed IGF2BP2 in LSCC serves as an oncogenic factor, promoting LSCC cell 
      proliferation and invasion in vitro and tumor growth in a xenograft tumor model 
      in vivo through facilitating CDK6 mRNA stabilization.
CI  - (c) 2023. Cell Death Differentiation Association (ADMC).
FAU - Tang, Xiaojun
AU  - Tang X
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital 
      of Central South University, Changsha, Hunan, China.
FAU - Tang, Qinglai
AU  - Tang Q
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital 
      of Central South University, Changsha, Hunan, China.
FAU - Li, Shisheng
AU  - Li S
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital 
      of Central South University, Changsha, Hunan, China.
FAU - Li, Mengmeng
AU  - Li M
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital 
      of Central South University, Changsha, Hunan, China.
FAU - Yang, Tao
AU  - Yang T
AUID- ORCID: 0000-0002-5575-4657
AD  - Department of Otolaryngology Head and Neck Surgery, The Second Xiangya Hospital 
      of Central South University, Changsha, Hunan, China. yt7065@csu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231010
PL  - United States
TA  - Cell Death Discov
JT  - Cell death discovery
JID - 101665035
PMC - PMC10564923
COIS- The authors declare no competing interests.
EDAT- 2023/10/11 00:42
MHDA- 2023/10/11 00:43
PMCR- 2023/10/10
CRDT- 2023/10/10 23:18
PHST- 2023/07/28 00:00 [received]
PHST- 2023/09/28 00:00 [accepted]
PHST- 2023/09/22 00:00 [revised]
PHST- 2023/10/11 00:43 [medline]
PHST- 2023/10/11 00:42 [pubmed]
PHST- 2023/10/10 23:18 [entrez]
PHST- 2023/10/10 00:00 [pmc-release]
AID - 10.1038/s41420-023-01669-7 [pii]
AID - 1669 [pii]
AID - 10.1038/s41420-023-01669-7 [doi]
PST - epublish
SO  - Cell Death Discov. 2023 Oct 10;9(1):371. doi: 10.1038/s41420-023-01669-7.