PMID- 37817016
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231123
IS  - 2731-9229 (Electronic)
IS  - 2731-9229 (Linking)
VI  - 18
IP  - 1
DP  - 2023 Oct 10
TI  - The shape-dependent inhibitory effect of rhein/silver nanocomposites on porcine 
      reproductive and respiratory syndrome virus.
PG  - 126
LID - 10.1186/s11671-023-03900-x [doi]
LID - 126
AB  - Traditional Chinese medicines (TCMs)/nanopreparations as viral antagonists 
      exhibited a structure-function correlation, i.e., the differences in surface 
      area/volume ratio caused by the variations in shape and size could result in 
      different biochemical properties and biological activities, suggesting an 
      important impact of morphology and structure on the antiviral activity of 
      TCM-based nanoparticles. However, few studies paid attention to this aspect. 
      Here, the effect of TCM-based nanoparticles with different morphologies on their 
      antiviral activity was explored by synthesizing rhein/silver nanocomposites 
      (Rhe@AgNPs) with spherical (S-Rhe/Ag) and linear (L-Rhe/Ag) morphologies, using 
      rhein (an active TCM ingredient) as a reducing agent and taking its self-assembly 
      advantage. Using porcine reproductive and respiratory syndrome virus (PRRSV) as a 
      model virus, the inhibitory effects of S-Rhe/Ag and L-Rhe/Ag on PRRSV were 
      compared. Results showed that the product morphology could be regulated by 
      varying pH values, and both S- and L-Rhe/Ag exhibited good dispersion and 
      stability, but with a smaller size for L-Rhe/Ag. Antiviral experiments revealed 
      that Rhe@AgNPs could effectively inhibit PRRSV infection, but the antiviral 
      effect was morphology-dependent. Compared with L-Rhe/Ag, S-Rhe/Ag could more 
      effectively inactivate PRRSV in vitro and antagonize its adsorption, invasion, 
      replication, and release stages. Mechanistic studies indicated that Rhe@AgNPs 
      could reduce the production of reactive oxygen species (ROS) induced by PRRSV 
      infection, and S-Rhe/Ag also had stronger ROS inhibitory effect. This work 
      confirmed the inhibitory effect of Rhe@AgNPs with different morphologies on PRRSV 
      and provided useful information for treating PRRSV infection with metal 
      nanoparticles synthesized from TCM ingredients.
CI  - (c) 2023. Springer Science+Business Media, LLC, part of Springer Nature.
FAU - Ren, Caifeng
AU  - Ren C
AD  - State Key Laboratory of Agricultural Microbiology, College of Resource and 
      Environment, College of Science, Huazhong Agricultural University, Wuhan, 430070, 
      People's Republic of China.
FAU - Ke, Qiyun
AU  - Ke Q
AD  - State Key Laboratory of Agricultural Microbiology, College of Veterinary 
      Medicine, Huazhong Agricultural University, Key Laboratory of Preventive 
      Veterinary Medicine in Hubei Province, Cooperative Innovation Center for 
      Sustainable Pig Production, Wuhan, 430070, People's Republic of China.
FAU - Fan, Xiaoxia
AU  - Fan X
AD  - State Key Laboratory of Agricultural Microbiology, College of Resource and 
      Environment, College of Science, Huazhong Agricultural University, Wuhan, 430070, 
      People's Republic of China.
FAU - Ning, Keke
AU  - Ning K
AD  - College of Science, Huazhong Agricultural University, Wuhan, 430070, People's 
      Republic of China.
FAU - Wu, Yuan
AU  - Wu Y
AD  - College of Science, Huazhong Agricultural University, Wuhan, 430070, People's 
      Republic of China.
FAU - Liang, Jiangong
AU  - Liang J
AD  - State Key Laboratory of Agricultural Microbiology, College of Resource and 
      Environment, College of Science, Huazhong Agricultural University, Wuhan, 430070, 
      People's Republic of China. liangjg@mail.hzau.edu.cn.
LA  - eng
GR  - 21904046/National Natural Science Foundation of China/
GR  - 22074048/National Natural Science Foundation of China/
GR  - 2022YFD1800305/National Key Research and Development Program of China/
PT  - Journal Article
DEP - 20231010
PL  - Switzerland
TA  - Discov Nano
JT  - Discover nano
JID - 9918540788706676
PMC - PMC10564707
OTO - NOTNLM
OT  - Antiviral mechanisms
OT  - Porcine reproductive and respiratory syndrome virus
OT  - Reactive oxygen species
OT  - Rhein
OT  - Silver nanocomposites
COIS- There are no competing interests to declare.
EDAT- 2023/10/11 00:42
MHDA- 2023/10/11 00:43
PMCR- 2023/10/10
CRDT- 2023/10/10 23:45
PHST- 2023/07/13 00:00 [received]
PHST- 2023/09/18 00:00 [accepted]
PHST- 2023/10/11 00:43 [medline]
PHST- 2023/10/11 00:42 [pubmed]
PHST- 2023/10/10 23:45 [entrez]
PHST- 2023/10/10 00:00 [pmc-release]
AID - 10.1186/s11671-023-03900-x [pii]
AID - 3900 [pii]
AID - 10.1186/s11671-023-03900-x [doi]
PST - epublish
SO  - Discov Nano. 2023 Oct 10;18(1):126. doi: 10.1186/s11671-023-03900-x.