PMID- 37817516
OWN - NLM
STAT- Publisher
LR  - 20231011
IS  - 2212-3946 (Electronic)
IS  - 1574-888X (Linking)
DP  - 2023 Oct 4
TI  - Mesenchymal Stem Cell-conditioned Medium Protecting Renal Tubular Epithelial 
      Cells by Inhibiting Hypoxia-inducible Factor-1alpha and Nuclear Receptor 
      Coactivator-1.
LID - 10.2174/011574888X247652230928064627 [doi]
AB  - BACKGROUND: Acute kidney injury (AKI) is characterized by inflammatory 
      infiltration and damage and death of renal tubular epithelial cells (RTECs), in 
      which hypoxia plays an important role. Deferoxamine (DFO) is a well-accepted 
      chemical hypoxia-mimetic agent. Mesenchymal stem cell-conditioned medium (MSC-CM) 
      can reduce local inflammation and repair tissue. In this study, we explored the 
      effect and molecular mechanism of MSC-CM-mediated protection of RTECs under 
      DFO-induced hypoxia. METHODS: Rat renal proximal tubule NRK-52E cells were 
      treated with different concentrations of DFO for 24 hours, followed by evaluation 
      of RTEC injury, using a Cell Counting Kit-8 (CCK-8) to detect cell viability and 
      western blotting to evaluate the expression of transforming growth factor- beta 1 
      (TGF-beta1), alpha-smooth muscle actin (alpha-SMA), and hypoxia-inducible factor-1 alpha 
      (HIF-1alpha) in NRK-52E cells. Then, three groups of NRK-52E cells were used in 
      experiments, including normal control (NC), 25 muM DFO, and 25 muM DFO + MSC-CM. 
      MSC-CM was obtained from the human umbilical cord. MSC-CM was used to culture 
      cells for 12 hours before DFO treatment, then fresh MSC-CM and 25 muM DFO were 
      added, and cells were cultured for another 24 hours before analysis. RESULTS: 
      Western blotting and cellular immunofluorescence staining showed culture of 
      NRK-52E cells in 25 muM DFO for 24 hours induced HIF-1alpha and nuclear receptor 
      coactivator-1 (NCoA-1), simulating hypoxia. MSC-CM could inhibit the DFO-induced 
      up-regulation of alpha-SMA, TGF-beta1, HIF-1alpha and NCoA-1. CONCLUSION: Our results 
      suggest that MSC-CM has a protective effect on RTECs by down-regulating HIF-1alpha 
      and NCoA-1, which may be the harmful factors in renal injury.
CI  - Copyright(c) Bentham Science Publishers; For any queries, please email at 
      epub@benthamscience.net.
FAU - Liao, Chunling
AU  - Liao C
AD  - Department of Nephrology, the Second Affiliated Hospital of Shantou University 
      Medical College, Shantou, 515041, China.
FAU - Weng, Wenjuan
AU  - Weng W
AD  - Department of Nephrology, the Second Affiliated Hospital of Shantou University 
      Medical College, Shantou, 515041, China.
FAU - Liu, Yiping
AU  - Liu Y
AD  - Department of Nephrology, the Second Affiliated Hospital of Shantou University 
      Medical College, Shantou, 515041, China.
FAU - Lin, Yongda
AU  - Lin Y
AD  - Department of Nephrology, the Second Affiliated Hospital of Shantou University 
      Medical College, Shantou, 515041, China.
FAU - Wang, Jiali
AU  - Wang J
AD  - Department of Nephrology, the Second Affiliated Hospital of Shantou University 
      Medical College, Shantou, 515041, China.
FAU - Zhou, Tianbiao
AU  - Zhou T
AD  - Department of Nephrology, the Second Affiliated Hospital of Shantou University 
      Medical College, Shantou, 515041, China.
LA  - eng
PT  - Journal Article
DEP - 20231004
PL  - United Arab Emirates
TA  - Curr Stem Cell Res Ther
JT  - Current stem cell research & therapy
JID - 101272517
SB  - IM
OTO - NOTNLM
OT  - HIF-1?
OT  - MSC-conditioned medium (MSC-CM)
OT  - NCoA-1
OT  - acute kidney injury (AKI)
OT  - deferoxamine (DFO)
OT  - renal tubular epithelial cells (RTECs)
EDAT- 2023/10/11 06:45
MHDA- 2023/10/11 06:45
CRDT- 2023/10/11 02:33
PHST- 2023/02/28 00:00 [received]
PHST- 2023/08/09 00:00 [revised]
PHST- 2023/08/24 00:00 [accepted]
PHST- 2023/10/11 06:45 [medline]
PHST- 2023/10/11 06:45 [pubmed]
PHST- 2023/10/11 02:33 [entrez]
AID - CSCR-EPUB-134759 [pii]
AID - 10.2174/011574888X247652230928064627 [doi]
PST - aheadofprint
SO  - Curr Stem Cell Res Ther. 2023 Oct 4. doi: 10.2174/011574888X247652230928064627.