PMID- 37820905
OWN - NLM
STAT- MEDLINE
DCOM- 20231127
LR  - 20231127
IS  - 1879-0003 (Electronic)
IS  - 0141-8130 (Linking)
VI  - 253
IP  - Pt 6
DP  - 2023 Dec 31
TI  - Anaerobic production and biosynthesis mechanism of exopolysaccharides in 
      Schizophyllum commune 20R-7-F01.
PG  - 127339
LID - S0141-8130(23)04236-8 [pii]
LID - 10.1016/j.ijbiomac.2023.127339 [doi]
AB  - Exopolysaccharides (EPS) produced by microorganisms play a vital role in 
      physiological and ecological processes. However, the mechanisms of EPS synthesis 
      and release in anaerobic environments remain poorly understood. Here, we provide 
      the first evidence of anaerobic EPS synthesis by the fungus Schizophyllum commune 
      20R-7-F01, isolated from coal-bearing sediments ~2.0 km below the seafloor. Under 
      anaerobic conditions, the fungus exhibited significantly higher specific EPS 
      production (1.57 times) than under aerobic conditions. Transcriptomic analysis 
      revealed 2057 differentially expressed genes (DEGs) in the strain cultured 
      anaerobically for 7 days compared to aerobically. Among these genes, 642 were 
      significantly upregulated, while 1415 were significantly downregulated, mainly 
      associated with carbon metabolism pathways. Genes involved in glycolysis and EPS 
      synthesis, including hexokinase (HK), phosphoglucomutase (PGM), and 
      (1 --> 3)-beta-glucan synthase (GLS), were significantly upregulated, while those 
      related to the TCA cycle, respiratory chain, and pentose phosphate pathway were 
      downregulated under anaerobic conditions. These findings highlight the 
      oxygen-dependent regulation of EPS synthesis and suggest that EPS may serve as a 
      key mechanism for fungal adaptation to anaerobic environments.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Ma, Yunan
AU  - Ma Y
AD  - State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 
      210023, China.
FAU - Zhao, Mengshi
AU  - Zhao M
AD  - State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 
      210023, China.
FAU - Zhou, Fan
AU  - Zhou F
AD  - State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 
      210023, China.
FAU - Liu, Xuan
AU  - Liu X
AD  - State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 
      210023, China.
FAU - Liu, Changhong
AU  - Liu C
AD  - State Key Laboratory of Pharmaceutical Biotechnology, Nanjing University, Nanjing 
      210023, China. Electronic address: chliu@nju.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231011
PL  - Netherlands
TA  - Int J Biol Macromol
JT  - International journal of biological macromolecules
JID - 7909578
RN  - 0 (beta-Glucans)
SB  - IM
MH  - *Schizophyllum/genetics/metabolism
MH  - Anaerobiosis
MH  - Glycolysis
MH  - *beta-Glucans/metabolism
OTO - NOTNLM
OT  - Anaerobic biosynthesis
OT  - Exopolysaccharides (EPS)
OT  - Fungi
OT  - Schizophyllum commune
OT  - Transcriptome
COIS- Declaration of competing interest The authors declare no declarations of 
      interest.
EDAT- 2023/10/12 00:43
MHDA- 2023/11/27 12:43
CRDT- 2023/10/11 19:38
PHST- 2023/06/09 00:00 [received]
PHST- 2023/10/01 00:00 [revised]
PHST- 2023/10/08 00:00 [accepted]
PHST- 2023/11/27 12:43 [medline]
PHST- 2023/10/12 00:43 [pubmed]
PHST- 2023/10/11 19:38 [entrez]
AID - S0141-8130(23)04236-8 [pii]
AID - 10.1016/j.ijbiomac.2023.127339 [doi]
PST - ppublish
SO  - Int J Biol Macromol. 2023 Dec 31;253(Pt 6):127339. doi: 
      10.1016/j.ijbiomac.2023.127339. Epub 2023 Oct 11.