PMID- 37821042
OWN - NLM
STAT- MEDLINE
DCOM- 20231106
LR  - 20231106
IS  - 1873-6424 (Electronic)
IS  - 0269-7491 (Linking)
VI  - 338
DP  - 2023 Dec 1
TI  - ROS meditated paralytic shellfish toxins production changes of Alexandrium 
      tamarense caused by microplastic particles.
PG  - 122702
LID - S0269-7491(23)01704-9 [pii]
LID - 10.1016/j.envpol.2023.122702 [doi]
AB  - A variety of studies have investigated the toxic effects of microplastics (MPs) 
      on microalgae, but few of them considered their influence on dinoflagellate 
      toxins production, which could cause significant ecological safety concerns in 
      coastal areas. This research investigated the impacts of 5 mug L(-1) and 
      5 mg L(-1) polystyrene (PS) MPs on the changes of paralytic shellfish toxins 
      (PSTs) production and their relationship with cellular oxidative stress of 
      Alexandrium tamarense, a common harmful algal blooms causative dinoflagellate. 
      The results showed elevation of reactive oxygen species (ROS) levels, activation 
      of antioxidant system and overproduction of PSTs were positively correlated under 
      PS MPs exposure (especially under 5 mg L(-1) PS MPs), and the PSTs changes were 
      eliminated by the ROS inhibitor. Further transcriptomic analysis revealed that 
      ROS could enhance biosynthesis of glutamate, providing raw materials for PSTs 
      precursor arginine, accompanied with enhanced acetyl-CoA and ATP production, 
      finally leading to the overproduction of PSTs. Moreover, the oxidative 
      intracellular environments might block the reduction process from STX to C1&C2, 
      leading to the increase of STX and decrease of C1&C2 proportions. This work 
      brings the first evidence that ROS could mediate PSTs production and compositions 
      of Alexandrium under MPs exposure, with important scientific and ecological 
      significance.
CI  - Copyright (c) 2023. Published by Elsevier Ltd.
FAU - Li, Danrui
AU  - Li D
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China.
FAU - Liu, Qian
AU  - Liu Q
AD  - Marine Science Research Institute of Shandong Province, Qingdao, 266104, China; 
      Qingdao Key Laboratory of Coastal Ecological Restoration and Security, Qingdao, 
      266104, China.
FAU - Zhao, Yirong
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Lv, Mengchen
AU  - Lv M
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China.
FAU - Tang, Xuexi
AU  - Tang X
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China.
FAU - Zhao, Yan
AU  - Zhao Y
AD  - College of Marine Life Sciences, Department of Marine Ecology, Ocean University 
      of China, Qingdao, 266003, China; Laboratory for Marine Ecology and Environmental 
      Science, Qingdao National Laboratory for Marine Science and Technology, Qingdao, 
      266071, China. Electronic address: zhaoyan@ouc.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231009
PL  - England
TA  - Environ Pollut
JT  - Environmental pollution (Barking, Essex : 1987)
JID - 8804476
RN  - 0 (Plastics)
RN  - 0 (Reactive Oxygen Species)
RN  - 0 (Microplastics)
RN  - 0 (Marine Toxins)
RN  - Alexandrium tamarense
SB  - IM
MH  - *Plastics/pharmacology
MH  - Reactive Oxygen Species
MH  - Microplastics/toxicity
MH  - *Dinoflagellida
MH  - Marine Toxins/toxicity
MH  - Shellfish
OTO - NOTNLM
OT  - Alexandrium tamarense
OT  - Microplastics
OT  - Paralytic shellfish toxins
OT  - ROS
OT  - Transcriptomic analysis
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/10/12 00:43
MHDA- 2023/11/06 06:42
CRDT- 2023/10/11 19:41
PHST- 2023/07/05 00:00 [received]
PHST- 2023/09/22 00:00 [revised]
PHST- 2023/10/04 00:00 [accepted]
PHST- 2023/11/06 06:42 [medline]
PHST- 2023/10/12 00:43 [pubmed]
PHST- 2023/10/11 19:41 [entrez]
AID - S0269-7491(23)01704-9 [pii]
AID - 10.1016/j.envpol.2023.122702 [doi]
PST - ppublish
SO  - Environ Pollut. 2023 Dec 1;338:122702. doi: 10.1016/j.envpol.2023.122702. Epub 
      2023 Oct 9.