PMID- 37826984 OWN - NLM STAT- MEDLINE DCOM- 20231106 LR - 20231106 IS - 1879-3231 (Electronic) IS - 0093-691X (Linking) VI - 213 DP - 2024 Jan 1 TI - Oxidative stress in donor mares for ovum pick-up delays embryonic development. PG - 109-113 LID - S0093-691X(23)00400-4 [pii] LID - 10.1016/j.theriogenology.2023.10.006 [doi] AB - The in vitro production of equine embryos via ovum pick-up (OPU) and intracytoplasmic sperm injection (ICSI) has increased rapidly. There is a marked effect of the individual mare on the outcome of OPU-ICSI, but little is known about the influence of the mare's health condition. This study aimed to investigate the potential associations between the concentrations of interleukin-6 (IL-6), reactive oxygen metabolites (d-ROMs), and biological antioxidant potential (BAP) in serum of oocytes' donor mares and the subsequent embryonic development. Just before OPU, a blood sample was collected from 28 Warmblood donor mares, that were subjected to a routine OPU-ICSI program. The serum concentrations of IL-6, d-ROMs, and BAP were assayed photometrically. The maturation, cleavage and blastocyst rate as well as the kinetics of blastocyst development were recorded. The average blastocyst rate was 24.68 +/- 5.16% and the average concentrations of IL-6, d-ROMs, and BAP were 519.59 +/- 157.08 pg/mL, 171.30 +/- 4.55 carratelli units (UCARR), and 2711.30 +/- 4.55 mumol/L, respectively. Serum concentrations of IL-6, d-ROMs, and BAP were not significantly different between mares yielding at least one blastocyst (552.68 +/- 235.18 pg/mL, 168.36 +/- 5.56 UCARR, and 2524.80 +/- 159.55 mumol/L) and mares yielding no blastocysts (468.47 +/- 179.99 pg/mL, 175.85 +/- 7.89 UCARR, and 2999.50 +/- 300.13 mumol/L, respectively). Serum concentrations of d-ROMs were significantly lower in mares with fast growing (at day 7-8 post ICSI; 148.10 +/- 8.13 UCARR) compared to those with slow growing blastocysts (>/= day 9 post ICSI; 179.41 +/- 4.89 UCARR; P = 0.003). Taken together, the serum concentration of IL-6, d-ROMs, and BAP do not determine the mare's ability to produce blastocysts in vitro. Although it may be questioned whether a single sample is representative of the mare's health status, changes in serum metabolites related to oxidative stress at the time of oocyte retrieval were linked to a delayed blastocyst development in a clinical OPU-ICSI outcome. CI - Copyright (c) 2023 Elsevier Inc. All rights reserved. FAU - Hedia, Mohamed AU - Hedia M AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Theriogenology Department, Faculty of Veterinary Medicine, Cairo University, Giza, Egypt; Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium. Electronic address: mohammedhedia@cu.edu.eg. FAU - Angel-Velez, Daniel AU - Angel-Velez D AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Research Group in Animal Sciences-INCA-CES, Universidad CES, 050021, Medellin, Colombia. FAU - Papas, Marion AU - Papas M AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium; Department of Clinical Sciences, Faculty of Veterinary Medicine, Utrecht University, Utrecht, the Netherlands. FAU - Peere, Sofie AU - Peere S AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - Gerits, Ilse AU - Gerits I AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - De Coster, Tine AU - De Coster T AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - Van den Branden, Emma AU - Van den Branden E AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - Govaere, Jan AU - Govaere J AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - Van Soom, Ann AU - Van Soom A AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. FAU - Leroy, Jo L M R AU - Leroy JLMR AD - Gamete Research Centre, Department of Veterinary Sciences, University of Antwerp, Wilrijk, Belgium. FAU - Smits, Katrien AU - Smits K AD - Department of Internal Medicine, Reproduction and Population Medicine, Faculty of Veterinary Medicine, Ghent University, Merelbeke, Belgium. Electronic address: Katrien.smits@ugent.be. LA - eng PT - Journal Article DEP - 20231008 PL - United States TA - Theriogenology JT - Theriogenology JID - 0421510 RN - 0 (Interleukin-6) RN - 0 (Antioxidants) SB - IM MH - Pregnancy MH - Animals MH - Horses MH - Female MH - Male MH - *Interleukin-6 MH - *Semen MH - Oxidative Stress MH - Blastocyst MH - Oocytes MH - Antioxidants MH - Embryonic Development OTO - NOTNLM OT - BAP OT - IL-6 OT - Mares OT - OPU-ICSI OT - Oxidative stress OT - r-ROMs COIS- Declaration of competing interest The authors declare no competing interests. EDAT- 2023/10/13 00:42 MHDA- 2023/11/06 06:42 CRDT- 2023/10/12 18:06 PHST- 2023/08/30 00:00 [received] PHST- 2023/10/06 00:00 [revised] PHST- 2023/10/07 00:00 [accepted] PHST- 2023/11/06 06:42 [medline] PHST- 2023/10/13 00:42 [pubmed] PHST- 2023/10/12 18:06 [entrez] AID - S0093-691X(23)00400-4 [pii] AID - 10.1016/j.theriogenology.2023.10.006 [doi] PST - ppublish SO - Theriogenology. 2024 Jan 1;213:109-113. doi: 10.1016/j.theriogenology.2023.10.006. Epub 2023 Oct 8.