PMID- 37827347
OWN - NLM
STAT- MEDLINE
DCOM- 20231106
LR  - 20231115
IS  - 1567-7257 (Electronic)
IS  - 1567-1348 (Linking)
VI  - 115
DP  - 2023 Nov
TI  - Molecular epidemiology and recombination of enterovirus D68 in China.
PG  - 105512
LID - S1567-1348(23)00110-7 [pii]
LID - 10.1016/j.meegid.2023.105512 [doi]
AB  - Enterovirus D68 (EV-D68), a member of Enterovirus genus of the Picornaviridae 
      family, mainly causes respiratory system-related diseases as well as neurological 
      complications in some patients. At present, there is no effective vaccine or 
      treatment for the virus. The aim of this research was to systematically analyse 
      the molecular epidemiology, recombination and changes in the epitope of EV-D68 in 
      China from 2008 to 2022. Through phylogenetic analysis based on VP1 sequences, it 
      was found that there was limited information about EV-D68 infection before 2011 
      and that EV-D68 infection was dominated by the A2 gene subtype from 2011 to 2013 
      and the B3 genotype from 2014 to 2018, during which A2 and B3 were coprevalent 
      and alternately prevalent. We also constructed a phylogenetic tree using the 
      EV-D68 full-length genome sequences, and the genotype of each sequence was 
      consistent with that of the VP1 sequence evolutionary tree. Recombination 
      analysis showed that MH341715 underwent intertypic recombination with the A2 
      genotype MH341729 at the 5' untranslated region (5'UTR) and that P1-P3 underwent 
      recombination with the B3 genotype MH341712. The capsid protein VP1 is one of the 
      most important structural proteins. In VP1, the BC-loop (89-105 amino acids) and 
      DE-loop (140-152 amino acids) are the most variable domains on the surface of the 
      virus and are associated with epitopes. In this study, it was found that the 
      dominant amino acid composition of the BC-loop and DE-loop continued to change 
      with the epidemic of the virus; the amino acid composition also differed in 
      different regions of the same genotypes. The ongoing genomic and molecular 
      epidemiology of EV-D68 remains important for predicting emergence of new viruses 
      and preventing major outbreaks of respiratory diseases.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier B.V. All rights reserved.
FAU - Shi, Yingying
AU  - Shi Y
AD  - Department of Immunology, School of Medicine, Jianghan University, Wuhan, Hubei 
      430056, China.
FAU - Liu, Yongjuan
AU  - Liu Y
AD  - Department of Central Laboratory, the Affiliated Lianyungang Hospital of Xuzhou 
      Medical University, Lianyungang, Jiangsu 222002, China.
FAU - Wu, Yanli
AU  - Wu Y
AD  - Department of Immunology, School of Medicine, Jianghan University, Wuhan, Hubei 
      430056, China.
FAU - Hu, Song
AU  - Hu S
AD  - Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University, 
      Wuhan, China.
FAU - Sun, Binlian
AU  - Sun B
AD  - Wuhan Institute of Biomedical Sciences, School of Medicine, Jianghan University, 
      Wuhan, China. Electronic address: binlian17@jhun.edu.cn.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231010
PL  - Netherlands
TA  - Infect Genet Evol
JT  - Infection, genetics and evolution : journal of molecular epidemiology and 
      evolutionary genetics in infectious diseases
JID - 101084138
RN  - 0 (Amino Acids)
SB  - IM
MH  - Humans
MH  - *Enterovirus D, Human/genetics
MH  - Molecular Epidemiology
MH  - Phylogeny
MH  - *Respiratory Tract Infections
MH  - China/epidemiology
MH  - *Enterovirus/genetics
MH  - *Enterovirus Infections/epidemiology
MH  - Recombination, Genetic
MH  - Amino Acids/genetics
OTO - NOTNLM
OT  - Antigenic evolution
OT  - Enterovirus D68
OT  - Molecular epidemiology
OT  - Phylogenetic tree
OT  - Recombination
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/10/13 00:43
MHDA- 2023/11/06 06:42
CRDT- 2023/10/12 19:27
PHST- 2023/08/10 00:00 [received]
PHST- 2023/10/05 00:00 [revised]
PHST- 2023/10/09 00:00 [accepted]
PHST- 2023/11/06 06:42 [medline]
PHST- 2023/10/13 00:43 [pubmed]
PHST- 2023/10/12 19:27 [entrez]
AID - S1567-1348(23)00110-7 [pii]
AID - 10.1016/j.meegid.2023.105512 [doi]
PST - ppublish
SO  - Infect Genet Evol. 2023 Nov;115:105512. doi: 10.1016/j.meegid.2023.105512. Epub 
      2023 Oct 10.