PMID- 37829206
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231020
IS  - 2589-0042 (Electronic)
IS  - 2589-0042 (Linking)
VI  - 26
IP  - 10
DP  - 2023 Oct 20
TI  - Heterogeneity of perivascular astrocyte endfeet depending on vascular regions in 
      the mouse brain.
PG  - 108010
LID - 10.1016/j.isci.2023.108010 [doi]
LID - 108010
AB  - Astrocytes interact with not only synapses but also brain blood vessels through 
      perivascular astrocyte endfeet (PV-AEF) to form the neurovascular unit (NVU). 
      However, PV-AEF components have not been fully identified. Here, we biochemically 
      isolated blood vessels from mouse brain homogenates and purified PV-AEF. The 
      purified PV-AEF were observed in different sizes, similar to PV-AEF on brain 
      blood vessels. Mass spectrometry analysis identified 9,762 proteins in the 
      purified PV-AEF, including cell adhesion molecules, nectin-2delta, Kirrel2, and 
      podoplanin. Immunofluorescence microscopic analysis revealed that nectin-2delta and 
      podoplanin were concentrated mainly in arteries/arterioles and veins/venules of 
      the mouse brain, whereas Kirrel2 was mainly in arteries/arterioles. Nectin-2alpha/delta, 
      Kirrel2, and podoplanin were preferentially observed in large sizes of the 
      purified PV-AEF. Furthermore, Kirrel2 potentially has cell adhesion activity of 
      cultured astrocytes. Collectively, these results indicate that PV-AEF have 
      heterogeneity in sizes and molecular components, implying different roles of 
      PV-AEF in NVU function depending on vascular regions.
CI  - (c) 2023 The Authors.
FAU - Kameyama, Takeshi
AU  - Kameyama T
AD  - Division of Pathogenetic Signaling, Department of Physiology and Cell Biology, 
      Kobe University Graduate School of Medicine, Kobe 650-0047, Japan.
AD  - Department of Immunology and Parasitology, Graduate School of Medicine, Tokushima 
      University, Tokushima 770-8503, Japan.
FAU - Miyata, Muneaki
AU  - Miyata M
AD  - Division of Pathogenetic Signaling, Department of Physiology and Cell Biology, 
      Kobe University Graduate School of Medicine, Kobe 650-0047, Japan.
AD  - Division of Pathogenetic Signaling, Institute of Advanced Medical Sciences, 
      Tokushima University, Tokushima 770-8503, Japan.
FAU - Shiotani, Hajime
AU  - Shiotani H
AD  - Division of Pathogenetic Signaling, Department of Physiology and Cell Biology, 
      Kobe University Graduate School of Medicine, Kobe 650-0047, Japan.
AD  - Division of Pathogenetic Signaling, Institute of Advanced Medical Sciences, 
      Tokushima University, Tokushima 770-8503, Japan.
FAU - Adachi, Jun
AU  - Adachi J
AD  - Laboratory of Proteomics for Drug Discovery, Center for Drug Design Research, 
      National Institute of Biomedical Innovation, Health and Nutrition, Osaka 
      567-0085, Japan.
AD  - Laboratory of Clinical and Analytical Chemistry, Center for Drug Design Research, 
      National Institute of Biomedical Innovation, Health and Nutrition, Osaka 
      567-0085, Japan.
FAU - Kakuta, Soichiro
AU  - Kakuta S
AD  - Laboratory of Morphology and Image Analysis, Biomedical Research Core Facilities, 
      Juntendo University Graduate School of Medicine, Tokyo 113-8421, Japan.
AD  - Department of Cellular Molecular Neuropathology, Juntendo University Graduate 
      School of Medicine, Tokyo 113-8421, Japan.
FAU - Uchiyama, Yasuo
AU  - Uchiyama Y
AD  - Department of Cellular Molecular Neuropathology, Juntendo University Graduate 
      School of Medicine, Tokyo 113-8421, Japan.
FAU - Mizutani, Kiyohito
AU  - Mizutani K
AD  - Division of Pathogenetic Signaling, Department of Physiology and Cell Biology, 
      Kobe University Graduate School of Medicine, Kobe 650-0047, Japan.
AD  - Division of Pathogenetic Signaling, Institute of Advanced Medical Sciences, 
      Tokushima University, Tokushima 770-8503, Japan.
FAU - Takai, Yoshimi
AU  - Takai Y
AD  - Division of Pathogenetic Signaling, Department of Physiology and Cell Biology, 
      Kobe University Graduate School of Medicine, Kobe 650-0047, Japan.
LA  - eng
PT  - Journal Article
DEP - 20230921
PL  - United States
TA  - iScience
JT  - iScience
JID - 101724038
PMC - PMC10565786
OTO - NOTNLM
OT  - Immunology
OT  - Molecular neuroscience
OT  - Vascular anatomy
COIS- The authors declare no competing interests.
EDAT- 2023/10/13 06:45
MHDA- 2023/10/13 06:46
PMCR- 2023/09/21
CRDT- 2023/10/13 04:00
PHST- 2023/02/08 00:00 [received]
PHST- 2023/07/14 00:00 [revised]
PHST- 2023/09/18 00:00 [accepted]
PHST- 2023/10/13 06:46 [medline]
PHST- 2023/10/13 06:45 [pubmed]
PHST- 2023/10/13 04:00 [entrez]
PHST- 2023/09/21 00:00 [pmc-release]
AID - S2589-0042(23)02087-4 [pii]
AID - 108010 [pii]
AID - 10.1016/j.isci.2023.108010 [doi]
PST - epublish
SO  - iScience. 2023 Sep 21;26(10):108010. doi: 10.1016/j.isci.2023.108010. eCollection 
      2023 Oct 20.