PMID- 37829557
OWN - NLM
STAT- MEDLINE
DCOM- 20231128
LR  - 20240123
IS  - 2090-0716 (Electronic)
IS  - 2090-0708 (Print)
IS  - 2090-0708 (Linking)
VI  - 2023
DP  - 2023
TI  - Prevalence of GCKR rs1260326 Variant in Subjects with Obesity Associated NAFLD 
      and T2DM: A Case-Control Study in South Punjab, Pakistan.
PG  - 6661858
LID - 10.1155/2023/6661858 [doi]
LID - 6661858
AB  - The glucokinase regulatory protein (GCKR) regulates glycogen metabolism and 
      insulin secretion, and the GCKR rs1260326 is a putative single nucleotide 
      polymorphism (SNP) associated with metabolic disorders including nonalcoholic 
      fatty liver disease (NAFLD) and type 2 diabetes mellitus (T2DM). This study was 
      conducted to investigate the genetic association of the GCKR rs1260326 in NAFLD 
      and T2DM in our population. NAFLD (n = 103), T2DM (n = 100), and control 
      (n = 100) samples were collected and genotyped for GCKR rs1260326 by tetra-arm 
      PCR. The genetic variant GCKR rs1260326 was significantly linked with NAFLD and 
      T2DM, while the GCKR rs1260326 was significantly associated with the progression 
      of obesity only in NAFLD subjects. The frequency of the C allele (mutant) was 
      higher in both NAFLD (f = 0.69) and T2DM (f = 0.66) subjects as compared to 
      healthy controls of NAFLD (0.52) and T2DM (f = 0.32). The frequency of the C 
      allele was also positively linked with the progression of obesity in both 
      diseases. The frequency of the C allele was 0.66, 0.67, and 0.74 in NAFLD normal 
      weight, overweight, and obese subjects, respectively, while the frequency of the 
      C allele was 0.60, 0.60, and 0.74 in T2DM in normal weight, overweight, and obese 
      subjects, respectively. Homozygous mutant (CC) was 53% in both NAFLD and T2DM 
      subjects, while heterozygous mutant (CT) was 15.53% in NAFLD and 22% in T2DM 
      subjects. Wild-type allele (TT) was 31.06% in NAFLD and 25% in T2DM subjects. In 
      conclusion, the GCKR rs1260326 is a highly prevalent SNP in NAFLD and T2DM 
      subjects, which possibly contributed to obesity, insulin resistance, and 
      metabolic disorders in our population.
CI  - Copyright (c) 2023 Tayyaba Nisar et al.
FAU - Nisar, Tayyaba
AU  - Nisar T
AD  - Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, 
      Multan 60800, Pakistan.
FAU - Arshad, Kashan
AU  - Arshad K
AD  - Department of Pediatric Endocrinology and Diabetes, Pediatric Unit-1, Allied 
      Hospital, Faisalabad 38800, Pakistan.
FAU - Abbas, Zahid
AU  - Abbas Z
AD  - Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, 
      Multan 60800, Pakistan.
FAU - Khan, Maira Ali
AU  - Khan MA
AD  - Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, 
      Multan 60800, Pakistan.
FAU - Safdar, Sohail
AU  - Safdar S
AD  - PHRC, Nishtar Hospital, Multan 60000, Pakistan.
FAU - Shaikh, Rehan Sadiq
AU  - Shaikh RS
AD  - Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, 
      Multan 60800, Pakistan.
AD  - Centre for Applied Molecular Biology, University of Punjab, Lahore, Pakistan.
FAU - Saeed, Ali
AU  - Saeed A
AUID- ORCID: 0000-0001-6255-4058
AD  - Institute of Molecular Biology and Biotechnology, Bahauddin Zakariya University, 
      Multan 60800, Pakistan.
AD  - Department of Pediatric Oncology and Medical Microbiology, University Medical 
      Center Groningen, University of Groningen, Groningen 9713, Netherlands.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231004
PL  - United States
TA  - J Obes
JT  - Journal of obesity
JID - 101526295
RN  - 0 (Adaptor Proteins, Signal Transducing)
RN  - 0 (GCKR protein, human)
RN  - 0 (glucokinase regulatory protein)
RN  - Punjabi people
SB  - IM
MH  - Humans
MH  - Adaptor Proteins, Signal Transducing/genetics
MH  - Case-Control Studies
MH  - *Diabetes Mellitus, Type 2/epidemiology/genetics
MH  - *Non-alcoholic Fatty Liver Disease/epidemiology/genetics
MH  - Obesity/epidemiology/genetics
MH  - Overweight
MH  - Pakistan/epidemiology
MH  - Polymorphism, Single Nucleotide/genetics
MH  - Prevalence
PMC - PMC10567336
COIS- The authors declare that they have no conflicts of interest.
EDAT- 2023/10/13 06:46
MHDA- 2023/11/01 12:44
PMCR- 2023/10/04
CRDT- 2023/10/13 04:05
PHST- 2023/07/10 00:00 [received]
PHST- 2023/09/02 00:00 [revised]
PHST- 2023/09/08 00:00 [accepted]
PHST- 2023/11/01 12:44 [medline]
PHST- 2023/10/13 06:46 [pubmed]
PHST- 2023/10/13 04:05 [entrez]
PHST- 2023/10/04 00:00 [pmc-release]
AID - 10.1155/2023/6661858 [doi]
PST - epublish
SO  - J Obes. 2023 Oct 4;2023:6661858. doi: 10.1155/2023/6661858. eCollection 2023.