PMID- 37830587
OWN - NLM
STAT- MEDLINE
DCOM- 20231101
LR  - 20231101
IS  - 2073-4409 (Electronic)
IS  - 2073-4409 (Linking)
VI  - 12
IP  - 19
DP  - 2023 Sep 28
TI  - Generation of Human Regulatory Dendritic Cells from Cryopreserved Healthy Donor 
      Cells and Hematopoietic Stem Cell Transplant Recipients.
LID - 10.3390/cells12192372 [doi]
LID - 2372
AB  - Acute graft versus host disease (GVHD) remains a significant complication 
      following hematopoietic stem cell transplant (HSCT), despite improved human 
      leukocyte antigen (HLA) matching and advances in prophylactic treatment regimens. 
      Previous studies have shown promising results for future regulatory dendritic 
      cell (DCreg) therapies in the amelioration of GVHD. This study evaluates the 
      effects of cryopreservation on the generation of DCreg, the generation of young 
      and older DCreg in serum-free media, and the feasibility of generating DCreg from 
      young and older HSCT patient monocytes. DCregs were generated in X-vivo 15 
      serum-free media from donor or patient monocytes. This study includes the use of 
      monocytes from young and older healthy, donor, and HSCT patients with varying 
      hematological diseases. Phenotypic differences in cell populations were assessed 
      via flow cytometry while pro-inflammatory and anti-inflammatory cytokine 
      production was evaluated in culture medium. The number of DCreg generated from 
      cryopreserved monocytes of healthy donors was not significantly different from 
      freshly isolated monocytes. DCreg generated from cryopreserved monocytes had 
      comparable levels of co-stimulatory molecule expression, inhibitory molecule 
      expression, and cytokine production as freshly isolated monocytes. Young and 
      older healthy donor monocytes generated similar numbers of DCreg with similar 
      cytokine production and phenotype. Although monocytes from older HSCT patients 
      generated significantly fewer DCreg, DCreg from young and older HSCT patients had 
      comparable phenotypes and cytokine production. Monocytes from young and older 
      myelodysplastic syndrome (MDS) patients generated reduced numbers of DCreg 
      compared to non-MDS-derived DCreg. We demonstrate that the cryopreservation of 
      monocytes from HSCT patients of varying hematological diseases allows for the 
      cost-effective generation of DCreg on an as-needed basis. Although the generation 
      of DCreg from MDS patients requires further assessment, these data support the 
      possibility of in vitro-generated DCreg as a therapy to reduce GVHD-associated 
      morbidity and mortality in young and older HSCT recipients.
FAU - Scroggins, Sabrina M
AU  - Scroggins SM
AUID- ORCID: 0000-0001-5842-0575
AD  - Department of Biomedical Sciences, University of Minnesota-Duluth, 1035 
      University Drive, 341 SMED, Duluth, MN 55812, USA.
FAU - Schlueter, Annette J
AU  - Schlueter AJ
AD  - Department of Pathology, University of Iowa, Iowa City, IA 52242, USA.
LA  - eng
GR  - K01 HL155240/HL/NHLBI NIH HHS/United States
GR  - KL2 TR002492/TR/NCATS NIH HHS/United States
GR  - UL1 TR002494/TR/NCATS NIH HHS/United States
PT  - Journal Article
PT  - Research Support, N.I.H., Extramural
DEP - 20230928
PL  - Switzerland
TA  - Cells
JT  - Cells
JID - 101600052
RN  - 0 (Culture Media, Serum-Free)
RN  - 0 (Cytokines)
SB  - IM
MH  - Humans
MH  - *Hematopoietic Stem Cell Transplantation/methods
MH  - Transplantation, Homologous/adverse effects
MH  - Culture Media, Serum-Free
MH  - *Graft vs Host Disease/etiology
MH  - *Myelodysplastic Syndromes/complications
MH  - Dendritic Cells
MH  - Cytokines
PMC - PMC10571850
OTO - NOTNLM
OT  - good manufacturing practices (GMPs)
OT  - graft versus host disease (GVHD)
OT  - hematopoietic stem-cell transplant (HSCT)
OT  - human
OT  - monocytes
OT  - regulatory dendritic cells (DCreg)
COIS- The authors declare no conflict of interest.
EDAT- 2023/10/13 12:43
MHDA- 2023/11/01 12:42
PMCR- 2023/09/28
CRDT- 2023/10/13 08:13
PHST- 2023/08/11 00:00 [received]
PHST- 2023/09/12 00:00 [revised]
PHST- 2023/09/23 00:00 [accepted]
PHST- 2023/11/01 12:42 [medline]
PHST- 2023/10/13 12:43 [pubmed]
PHST- 2023/10/13 08:13 [entrez]
PHST- 2023/09/28 00:00 [pmc-release]
AID - cells12192372 [pii]
AID - cells-12-02372 [pii]
AID - 10.3390/cells12192372 [doi]
PST - epublish
SO  - Cells. 2023 Sep 28;12(19):2372. doi: 10.3390/cells12192372.