PMID- 37831473
OWN - NLM
STAT- Publisher
LR  - 20231013
IS  - 1899-5276 (Print)
IS  - 1899-5276 (Linking)
DP  - 2023 Oct 13
TI  - Effects of myostatin gene knockout on white fat browning and related gene 
      expression in type 2 diabetic mice.
LID - 10.17219/acem/171300 [doi]
AB  - BACKGROUND: Myostatin (Mstn) plays an important role in adipocyte growth, 
      differentiation and metabolism, leading to the development of obesity. 
      OBJECTIVES: We aimed to explore the effect of Mstn on white fat browning in a 
      mouse model of type 2 diabetes mellitus (T2DM). MATERIAL AND METHODS: Twelve 
      wild-type (WT), 12 heterozygous (Mstn(+/-)) and 12 homozygous (Mstn(-/-)) male 
      mice were randomly divided into 6 groups: WT, Mstn(+/-), Mstn(-/-), WT+DM, 
      Mstn(+/-)+DM, and Mstn(-/-)+DM. The first 3 groups were fed normal chow, while 
      the last 3 were fed high-fat diet and administered streptozotocin to generate 
      T2DM. Subsequently, body mass, length, and white and brown fat masses were 
      measured, after which Lee's index, white-brown ratio and fat index were 
      calculated. The serum free fatty acid (FFA) levels were detected using 
      enzyme-linked immunosorbent assay (ELISA). Hematoxylin and eosin (H&E) staining 
      was used to analyze white and brown fat cell morphology. The relative expression 
      levels of peroxisome proliferator-activated receptor-gamma (PPARgamma), peroxisome 
      proliferator-activated receptor-gamma coactivator-1 alpha (PGC-1alpha), uncoupling 
      protein 1 (UCP1), and cluster of differentiation 137 (CD137) protein were 
      determined with western blotting. RESULTS: The Mstn(-/-) group displayed higher 
      levels of PPARgamma, PGC-1alpha and CD137 proteins in white and brown fat compared to the 
      WT and Mstn(+/-) groups, while the expression level of UCP1 protein in the 
      Mstn(-/-) group was higher than in the WT group. The expression levels of PPARgamma, 
      PGC-1alpha, UCP1, and CD137 proteins in the WT+DM group were lower than in the WT 
      group. Moreover, PPARgamma, PGC-1alpha, UCP1, and CD137 proteins were more highly 
      expressed in the Mstn(-/-)+DM group compared to the WT+DM and Mstn(+/-)+DM 
      groups. CONCLUSIONS: The Mstn gene inhibition antagonizes obesity phenotypes, 
      such as white fat accumulation and lipid metabolism derangement caused by T2DM, 
      thus promoting white fat browning.
FAU - Cheng, Jingwei
AU  - Cheng J
AD  - School of Physical Education, Henan University, Kaifeng, China.
AD  - School of Sports Science, Kyonggi University, Suwon, South Korea.
FAU - Lee, Jaewoo
AU  - Lee J
AD  - Graduate School, Kyonggi University, Suwon, South Korea.
FAU - Liu, Yangqing
AU  - Liu Y
AD  - Department of Editing, Henan Provincial People's Hospital, Zhengzhou University 
      People's Hospital, China.
FAU - Wang, Yanfang
AU  - Wang Y
AD  - Department of Endocrinology, Henan Provincial People's Hospital, Zhengzhou 
      University People's Hospital, China.
FAU - Duan, Mingtao
AU  - Duan M
AD  - School of Sports Science, Kyonggi University, Suwon, South Korea.
FAU - Zeng, Zhen
AU  - Zeng Z
AD  - Graduate School, Kyonggi University, Suwon, South Korea.
LA  - eng
PT  - Journal Article
DEP - 20231013
PL  - Poland
TA  - Adv Clin Exp Med
JT  - Advances in clinical and experimental medicine : official organ Wroclaw Medical 
      University
JID - 101138582
SB  - IM
OTO - NOTNLM
OT  - brown adipose tissue
OT  - diabetes mellitus type 2
OT  - mice
OT  - myostatin
OT  - obesity
EDAT- 2023/10/13 12:44
MHDA- 2023/10/13 12:44
CRDT- 2023/10/13 11:42
PHST- 2022/04/21 00:00 [received]
PHST- 2023/05/07 00:00 [revised]
PHST- 2023/08/16 00:00 [accepted]
PHST- 2023/10/13 12:44 [medline]
PHST- 2023/10/13 12:44 [pubmed]
PHST- 2023/10/13 11:42 [entrez]
AID - 10.17219/acem/171300 [doi]
PST - aheadofprint
SO  - Adv Clin Exp Med. 2023 Oct 13. doi: 10.17219/acem/171300.