PMID- 37831588
OWN - NLM
STAT- MEDLINE
DCOM- 20231107
LR  - 20231107
IS  - 0261-1929 (Print)
IS  - 0261-1929 (Linking)
VI  - 51
IP  - 6
DP  - 2023 Nov
TI  - Optimisation of a Method for the Differentiation of Human Umbilical Cord-derived 
      Mesenchymal Stem Cells Toward Renal Epithelial-like Cells.
PG  - 363-375
LID - 10.1177/02611929231207774 [doi]
AB  - Human umbilical cord-derived mesenchymal stem cells (hucMSCs) can differentiate 
      into multiple cell lineages, but few methods have been developed to generate 
      kidney lineage cells. Due to their human origin, pluripotent nature and 
      immunomodulatory properties, these stem cells are attractive candidates for 
      clinical applications such as the repair or regeneration of damaged organs. This 
      study evaluated the renal differentiation potential of hucMSCs, when exposed for 
      10 days to optimised concentrations of retinoic acid, activin-A and bone 
      morphogenetic protein-7 (BMP-7) in various combinations, with and without the 
      priming of the cells with a Wnt signalling pathway activator (CHIR99021). The 
      hucMSCs were isolated and characterised according to surface marker expression 
      (CD73, CD90, CD44, CD146 and CD8) and tri-lineage differentiation potential. The 
      expression of key marker genes (OSR1, TBXT, HOXA13, SIX2, PAX2, KRT18 and ZO1) 
      was examined by qRT-PCR. Specific marker protein expression (E-cadherin, 
      cytokeratin-8 and cytokeratin-19) was analysed by immunocytochemistry. 
      CHIR99021-primed cells treated with the retinoic acid, activin-A and BMP-7 
      cocktail showed epithelial cell-like differentiation - i.e. distinct phenotypic 
      changes, as well as upregulated gene and protein expression, were observed that 
      were consistent with an epithelial cell phenotype. Thus, our results showed that 
      hucMSCs can efficiently differentiate into renal epithelial-like cells. This work 
      may help in the development of focused therapeutic strategies, in which 
      lineage-defined human stem cells can be used for renal regeneration.
FAU - Habib, Rakhshinda
AU  - Habib R
AD  - Dow Research Institute of Biotechnology and Biomedical Sciences, Dow University 
      of Health Sciences (Ojha campus), Karachi, Pakistan. RINGGOLD: 66818
FAU - Fahim, Shumaila
AU  - Fahim S
AD  - Dow Research Institute of Biotechnology and Biomedical Sciences, Dow University 
      of Health Sciences (Ojha campus), Karachi, Pakistan. RINGGOLD: 66818
FAU - Wahid, Mohsin
AU  - Wahid M
AD  - Department of Pathology, Dow International Medical College, Dow University of 
      Health Sciences (Ojha campus), Karachi, Pakistan. RINGGOLD: 66818
FAU - Ainuddin, Jahanara
AU  - Ainuddin J
AD  - Department of Gynaecology and Obstetrics, Dow University Hospital, Karachi, 
      Pakistan.
LA  - eng
PT  - Journal Article
DEP - 20231013
PL  - England
TA  - Altern Lab Anim
JT  - Alternatives to laboratory animals : ATLA
JID - 8110074
RN  - 0 (Bone Morphogenetic Protein 7)
RN  - 5688UTC01R (Tretinoin)
RN  - 104625-48-1 (Activins)
SB  - IM
MH  - Humans
MH  - *Bone Morphogenetic Protein 7/metabolism
MH  - Umbilical Cord
MH  - Cell Differentiation
MH  - *Mesenchymal Stem Cells/metabolism
MH  - Epithelial Cells
MH  - Tretinoin/metabolism
MH  - Activins/pharmacology/metabolism
MH  - Cells, Cultured
OTO - NOTNLM
OT  - cell culture
OT  - differentiation
OT  - human umbilical cord-derived mesenchymal stem cells
OT  - renal epithelial-like cells
OT  - small molecule
COIS- Declaration of Conflicting InterestsThe author(s) declared no potential conflicts 
      of interest with respect to the research, authorship, and/or publication of this 
      article.
EDAT- 2023/10/13 18:43
MHDA- 2023/11/07 06:46
CRDT- 2023/10/13 12:33
PHST- 2023/11/07 06:46 [medline]
PHST- 2023/10/13 18:43 [pubmed]
PHST- 2023/10/13 12:33 [entrez]
AID - 10.1177/02611929231207774 [doi]
PST - ppublish
SO  - Altern Lab Anim. 2023 Nov;51(6):363-375. doi: 10.1177/02611929231207774. Epub 
      2023 Oct 13.