PMID- 37833937
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231023
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 19
DP  - 2023 Sep 23
TI  - Expression of Cytoskeletal Proteins (GFAP, Vimentin), Proapoptotic Protein 
      (Caspase-3) and Protective Protein (S100) in the Epileptic Focus in Adults and 
      Children with Drug-Resistant Temporal Lobe Epilepsy Associated with Focal 
      Cortical Dysplasia.
LID - 10.3390/ijms241914490 [doi]
LID - 14490
AB  - The European Commission of the International League Against Epilepsy (ILAE) has 
      identified glial mechanisms of seizures and epileptogenesis as top research 
      priorities. The aim of our study was to conduct a comparative analysis of the 
      expression levels of cytoskeletal proteins (glial fibrillar acidic protein (GFAP) 
      and vimentin), protective protein S100, and proapoptotic caspase-3 protein in 
      patients with drug-resistant epilepsy (DRE) associated with focal cortical 
      dysplasia (FCD). We aimed to investigate how the expression levels of these 
      proteins depend on age (both in children and adults), gender, and disease 
      duration, using immunohistochemistry. Nonparametric statistical methods were 
      employed for data analysis. In the epileptic focus area of the cortex and white 
      matter in patients with FCD-associated temporal lobe DRE, a higher level of 
      expression of these proteins was observed. Age and gender differences were found 
      for vimentin and S100. In the early stages of disease development, there was a 
      compensatory sequential increase in the expression of cytoskeletal and protective 
      proteins. In patients with DRE, depending on the disease duration, patterns of 
      development of neurodegeneration were noted, which is accompanied by apoptosis of 
      gliocytes. These results provide insights into epilepsy mechanisms and may 
      contribute to improving diagnostic and treatment approaches.
FAU - Sitovskaya, Darya
AU  - Sitovskaya D
AUID- ORCID: 0000-0001-9721-3827
AD  - Polenov Neurosurgical Institute-Branch of Almazov National Medical Research 
      Centre, 197341 St. Petersburg, Russia.
AD  - Department of Pathology with a Course of Forensic Medicine Named after D.D. 
      Lochov, St. Petersburg State Pediatric Medical University, 194100 St. Petersburg, 
      Russia.
FAU - Zabrodskaya, Yulia
AU  - Zabrodskaya Y
AD  - Polenov Neurosurgical Institute-Branch of Almazov National Medical Research 
      Centre, 197341 St. Petersburg, Russia.
AD  - Department of Pathology, Mechnikov North-West State Medical University, 191015 
      St. Petersburg, Russia.
FAU - Parshakov, Petr
AU  - Parshakov P
AUID- ORCID: 0000-0002-1805-2680
AD  - International Laboratory of Intangible-Driven Economy, National Research 
      University Higher School of Economics, 614070 Perm, Russia.
FAU - Sokolova, Tatyana
AU  - Sokolova T
AD  - Polenov Neurosurgical Institute-Branch of Almazov National Medical Research 
      Centre, 197341 St. Petersburg, Russia.
FAU - Kudlay, Dmitry
AU  - Kudlay D
AUID- ORCID: 0000-0003-1878-4467
AD  - Department of Pharmacology, Institute of Pharmacy, I.M. Sechenov First Moscow 
      State Medical University, 119991 Moscow, Russia.
AD  - NRC Institute of Immunology FMBA of Russia, 115552 Moscow, Russia.
FAU - Starshinova, Anna
AU  - Starshinova A
AUID- ORCID: 0000-0002-9023-6986
AD  - Polenov Neurosurgical Institute-Branch of Almazov National Medical Research 
      Centre, 197341 St. Petersburg, Russia.
FAU - Samochernykh, Konstantin
AU  - Samochernykh K
AD  - Polenov Neurosurgical Institute-Branch of Almazov National Medical Research 
      Centre, 197341 St. Petersburg, Russia.
LA  - eng
PT  - Journal Article
DEP - 20230923
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 0 (Vimentin)
RN  - 0 (Cytoskeletal Proteins)
RN  - 0 (Apoptosis Regulatory Proteins)
RN  - EC 3.4.22.- (Caspase 3)
RN  - 0 (Glial Fibrillary Acidic Protein)
SB  - IM
MH  - Humans
MH  - Adult
MH  - Child
MH  - *Epilepsy, Temporal Lobe/metabolism
MH  - Vimentin/genetics/metabolism
MH  - Cytoskeletal Proteins/metabolism
MH  - *Focal Cortical Dysplasia
MH  - Apoptosis Regulatory Proteins/metabolism
MH  - Caspase 3/metabolism
MH  - *Epilepsy/metabolism
MH  - *Drug Resistant Epilepsy
MH  - Temporal Lobe/metabolism
MH  - Glial Fibrillary Acidic Protein/metabolism
MH  - Retrospective Studies
PMC - PMC10572279
OTO - NOTNLM
OT  - GFAP
OT  - S100
OT  - adult
OT  - apoptosis
OT  - caspase-3
OT  - children
OT  - drug-resistant epilepsy
OT  - focal cortical dysplasia
OT  - immunohistochemistry
OT  - neurodegeneration
OT  - vimentin
COIS- The authors declare no conflict of interest.
EDAT- 2023/10/14 10:44
MHDA- 2023/10/23 01:18
PMCR- 2023/09/23
CRDT- 2023/10/14 01:00
PHST- 2023/07/29 00:00 [received]
PHST- 2023/09/01 00:00 [revised]
PHST- 2023/09/10 00:00 [accepted]
PHST- 2023/10/23 01:18 [medline]
PHST- 2023/10/14 10:44 [pubmed]
PHST- 2023/10/14 01:00 [entrez]
PHST- 2023/09/23 00:00 [pmc-release]
AID - ijms241914490 [pii]
AID - ijms-24-14490 [pii]
AID - 10.3390/ijms241914490 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Sep 23;24(19):14490. doi: 10.3390/ijms241914490.