PMID- 37834266
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231023
IS  - 1422-0067 (Electronic)
IS  - 1422-0067 (Linking)
VI  - 24
IP  - 19
DP  - 2023 Oct 1
TI  - Synthesis, Physicochemical Characterization using a Facile Validated HPLC 
      Quantitation Analysis Method of 4-Chloro-phenylcarbamoyl-methyl Ciprofloxacin and 
      Its Biological Investigations.
LID - 10.3390/ijms241914818 [doi]
LID - 14818
AB  - A novel derivative of ciprofloxacin (Cpx) was synthesized and characterized using 
      various analytical techniques, including FT-IR spectroscopy, UV-Vis spectroscopy, 
      TEM and SEM analysis, (1)H NMR, (13)C NMR, and HPLC analysis. The newly prepared 
      Cpx derivative (Cpx-Drv) exhibited significantly enhanced antibacterial 
      properties compared to Cpx itself. In particular, Cpx-Drv demonstrated a 51% 
      increase in antibacterial activity against S. aureus and a 30% improvement 
      against B. subtilis. It displayed potent inhibitory effects on topoisomerases II 
      (DNA gyrase and topoisomerase IV) as potential molecular targets, with IC50 
      values of 6.754 and 1.913 microg/mL, respectively, in contrast to Cpx, which had IC50 
      values of 2.125 and 0.821 microg/mL, respectively. Docking studies further supported 
      these findings, showing that Cpx-Drv exhibited stronger binding interactions with 
      the gyrase enzyme (PDB ID: 2XCT) compared to the parent Cpx, with binding 
      affinities of -10.3349 and -7.7506 kcal/mole, respectively.
FAU - Al-Hakkani, Mostafa F
AU  - Al-Hakkani MF
AUID- ORCID: 0000-0003-0004-8939
AD  - Department of Research, Development, and Stability, UP Pharma, Industrial Zone, 
      Arab El Awamer, Abnoub 76, Assiut 71745, Egypt.
FAU - Ahmed, Nourhan
AU  - Ahmed N
AD  - Department of Research, Development, and Stability, UP Pharma, Industrial Zone, 
      Arab El Awamer, Abnoub 76, Assiut 71745, Egypt.
FAU - Abbas, Alaa A
AU  - Abbas AA
AD  - Department of Research, Development, and Stability, UP Pharma, Industrial Zone, 
      Arab El Awamer, Abnoub 76, Assiut 71745, Egypt.
FAU - Hassan, Mohammad H A
AU  - Hassan MHA
AD  - Department of Medical Laboratory Technology, Higher Technological Institute for 
      Applied Health Sciences in Minya, Minya 71511, Egypt.
FAU - Aziz, Hossameldin A
AU  - Aziz HA
AD  - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, New Valley 
      University, New Valley 72511, Egypt.
FAU - Elshamsy, Ali M
AU  - Elshamsy AM
AD  - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Deraya University, 
      Mina, New Minia 61768, Egypt.
FAU - Khalifa, Hazim O
AU  - Khalifa HO
AUID- ORCID: 0000-0001-9861-9693
AD  - Department of Veterinary Medicine, College of Agriculture and Veterinary 
      Medicine, United Arab Emirates University, Al Ain P.O. Box 1555, United Arab 
      Emirates.
AD  - Department of Pharmacology, Faculty of Veterinary Medicine, Kafr Elsheikh 
      University, Kafr El Sheikh 33516, Egypt.
FAU - Abdelshakour, Mohamed A
AU  - Abdelshakour MA
AD  - Department of Pharmaceutical Analytical Chemistry, Faculty of Pharmacy, Sohag 
      University, Sohag 82524, Egypt.
FAU - Saddik, Mohammed S
AU  - Saddik MS
AUID- ORCID: 0000-0001-5236-7419
AD  - Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag 
      University, Sohag 82524, Egypt.
FAU - Elsayed, Mahmoud M A
AU  - Elsayed MMA
AUID- ORCID: 0000-0001-9547-2243
AD  - Department of Pharmaceutics and Clinical Pharmacy, Faculty of Pharmacy, Sohag 
      University, Sohag 82524, Egypt.
FAU - Sabet, Marwa A
AU  - Sabet MA
AUID- ORCID: 0000-0001-9557-1153
AD  - Department of Microbiology and Immunology, Faculty of Pharmacy, Sphinx 
      University, New-Assiut 71684, Egypt.
FAU - El-Mokhtar, Mohamed A
AU  - El-Mokhtar MA
AUID- ORCID: 0000-0003-1943-8419
AD  - Department of Medical Microbiology and Immunology, Faculty of Medicine, Assiut 
      University, Assiut 71515, Egypt.
FAU - Alsehli, Mosa
AU  - Alsehli M
AD  - Department of Chemistry, College of Science, Taibah University, Madinah P.O. Box 
      344, Saudi Arabia.
FAU - Amin, M S
AU  - Amin MS
AD  - Department of Chemistry, College of Science, Taibah University, Madinah P.O. Box 
      344, Saudi Arabia.
AD  - Chemistry Department, Faculty of science, Ain Shams University, Cairo 11566, 
      Egypt.
FAU - Abu-Dief, Ahmed M
AU  - Abu-Dief AM
AD  - Department of Chemistry, College of Science, Taibah University, Madinah P.O. Box 
      344, Saudi Arabia.
AD  - Department of Chemistry, Faculty of Science, Sohag University, Sohag 82524, 
      Egypt.
FAU - Mohammed, Hamada H H
AU  - Mohammed HHH
AUID- ORCID: 0000-0002-8649-7534
AD  - Department of Pharmaceutical Chemistry, Faculty of Pharmacy, Sohag University, 
      Sohag 82524, Egypt.
LA  - eng
GR  - 445-9-333/Taibah University/
PT  - Journal Article
DEP - 20231001
PL  - Switzerland
TA  - Int J Mol Sci
JT  - International journal of molecular sciences
JID - 101092791
RN  - 5E8K9I0O4U (Ciprofloxacin)
RN  - 0 (Anti-Bacterial Agents)
RN  - EC 5.99.1.3 (DNA Gyrase)
RN  - 0 (Topoisomerase II Inhibitors)
SB  - IM
MH  - *Ciprofloxacin/pharmacology/chemistry
MH  - *Staphylococcus aureus
MH  - Chromatography, High Pressure Liquid
MH  - Spectroscopy, Fourier Transform Infrared
MH  - Microbial Sensitivity Tests
MH  - Anti-Bacterial Agents/chemistry
MH  - DNA Gyrase
MH  - Molecular Docking Simulation
MH  - Topoisomerase II Inhibitors/pharmacology/chemistry
PMC - PMC10573198
OTO - NOTNLM
OT  - DNA gyrase
OT  - HPLC
OT  - antibacterial
OT  - anticancer
OT  - ciprofloxacin
OT  - docking studies
OT  - validation
COIS- The authors declare no conflict of interest.
EDAT- 2023/10/14 10:47
MHDA- 2023/10/23 01:18
PMCR- 2023/10/01
CRDT- 2023/10/14 01:03
PHST- 2023/09/08 00:00 [received]
PHST- 2023/09/26 00:00 [revised]
PHST- 2023/09/30 00:00 [accepted]
PHST- 2023/10/23 01:18 [medline]
PHST- 2023/10/14 10:47 [pubmed]
PHST- 2023/10/14 01:03 [entrez]
PHST- 2023/10/01 00:00 [pmc-release]
AID - ijms241914818 [pii]
AID - ijms-24-14818 [pii]
AID - 10.3390/ijms241914818 [doi]
PST - epublish
SO  - Int J Mol Sci. 2023 Oct 1;24(19):14818. doi: 10.3390/ijms241914818.