PMID- 37837724
OWN - NLM
STAT- MEDLINE
DCOM- 20231102
LR  - 20240328
IS  - 1873-3778 (Electronic)
IS  - 0021-9673 (Linking)
VI  - 1710
DP  - 2023 Nov 8
TI  - Determination of tobacco-specific nitrosamines in tobacco and mainstream 
      cigarette smoke using one-step clean-up coupled with liquid chromatography- 
      tandem mass spectrometry.
PG  - 464436
LID - S0021-9673(23)00661-1 [pii]
LID - 10.1016/j.chroma.2023.464436 [doi]
AB  - A method for determining tobacco-specific nitrosamines (TSNAs) in tobacco and 
      cigarette smoke using liquid chromatography-tandem mass spectrometry was 
      established. The established method amended the deficiencies that exist in 
      current mainstream methods. In this method, TSNAs in tobacco and cigarette smoke 
      were extracted by water. The aqueous extract was then extracted by 
      dichloromethane, and the extract could be analyzed by liquid 
      chromatography-tandem mass spectrometry after a solvent replacement. This method 
      was used to analyze flue-cured tobacco samples, and the response of the target 
      compounds was about 10 times higher than that of the ammonium acetate extraction 
      method. When analyzing cigarette smoke samples, the response strength and 
      chromatographic peak purity of the target compounds were also significantly 
      improved. The proposed method exhibited good linearities for both tobacco and 
      cigarette smoke samples (r(2) > 0.99). The limits of detection (LODs) for tobacco 
      and cigarette smoke samples were 0.2-1.0 ng/g and 0.1-0.3 ng/cigarette, 
      respectively. Additionally, this method exhibited desirable accuracy and 
      precision. The TSNAs recovery values from tobacco and cigarette smoke samples 
      ranged from 95.7 % to 107.7 % with inter- and intra-day relative standard 
      deviations (RSDs) of less than 7.4 %. This method is simple, effective, and has 
      wide adaptability. It is a useful upgrade to the existing methods for analyzing 
      TSNAs in tobacco and cigarette smoke.
CI  - Copyright (c) 2023. Published by Elsevier B.V.
FAU - Li, Yong
AU  - Li Y
AD  - Yunnan academy of tobacco agricultural sciences, No. 33 Yuantong street, Wuhua 
      district, Kunming, Yunnan 650021, China.
FAU - Pang, Tao
AU  - Pang T
AD  - Yunnan academy of tobacco agricultural sciences, No. 33 Yuantong street, Wuhua 
      district, Kunming, Yunnan 650021, China. Electronic address: baggiopt@126.com.
FAU - Shi, Junli
AU  - Shi J
AD  - Yunnan academy of tobacco agricultural sciences, No. 33 Yuantong street, Wuhua 
      district, Kunming, Yunnan 650021, China.
FAU - Liu, Xingyu
AU  - Liu X
AD  - Shanghai tobacco group Beijing cigarette factory, Beijing 101121, China.
FAU - Xu, Zhaoli
AU  - Xu Z
AD  - Yunnan academy of tobacco agricultural sciences, No. 33 Yuantong street, Wuhua 
      district, Kunming, Yunnan 650021, China.
FAU - Song, Zhongbang
AU  - Song Z
AD  - Yunnan academy of tobacco agricultural sciences, No. 33 Yuantong street, Wuhua 
      district, Kunming, Yunnan 650021, China.
FAU - Xie, He
AU  - Xie H
AD  - Yunnan academy of tobacco agricultural sciences, No. 33 Yuantong street, Wuhua 
      district, Kunming, Yunnan 650021, China.
LA  - eng
PT  - Journal Article
DEP - 20231006
PL  - Netherlands
TA  - J Chromatogr A
JT  - Journal of chromatography. A
JID - 9318488
RN  - 0 (Nitrosamines)
SB  - IM
MH  - *Nitrosamines/analysis
MH  - Tandem Mass Spectrometry/methods
MH  - *Cigarette Smoking
MH  - Reproducibility of Results
MH  - Chromatography, Liquid
OTO - NOTNLM
OT  - Cigarette smoke
OT  - Liquid chromatography-tandem mass spectrometry
OT  - Liquid-liquid extraction
OT  - Tobacco
OT  - Tobacco-specific nitrosamines
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/10/15 05:46
MHDA- 2023/11/02 12:44
CRDT- 2023/10/14 18:02
PHST- 2023/06/01 00:00 [received]
PHST- 2023/09/27 00:00 [revised]
PHST- 2023/10/05 00:00 [accepted]
PHST- 2023/11/02 12:44 [medline]
PHST- 2023/10/15 05:46 [pubmed]
PHST- 2023/10/14 18:02 [entrez]
AID - S0021-9673(23)00661-1 [pii]
AID - 10.1016/j.chroma.2023.464436 [doi]
PST - ppublish
SO  - J Chromatogr A. 2023 Nov 8;1710:464436. doi: 10.1016/j.chroma.2023.464436. Epub 
      2023 Oct 6.