PMID- 37840200
OWN - NLM
STAT- MEDLINE
DCOM- 20240130
LR  - 20240206
IS  - 1521-4141 (Electronic)
IS  - 0014-2980 (Linking)
VI  - 54
IP  - 1
DP  - 2024 Jan
TI  - Detection of dendritic cell subsets in the tumor microenvironment by multiplex 
      immunohistochemistry.
PG  - e2350616
LID - 10.1002/eji.202350616 [doi]
AB  - Dendritic cells (DCs) are essential in antitumor immunity. In humans, three main 
      DC subsets are defined: two types of conventional DCs (cDC1s and cDC2s) and 
      plasmacytoid DCs (pDCs). To study DC subsets in the tumor microenvironment (TME), 
      it is important to correctly identify them in tumor tissues. Tumor-derived DCs 
      are often analyzed in cell suspensions in which spatial information about DCs 
      which can be important to determine their function within the TME is lost. 
      Therefore, we developed the first standardized and optimized multiplex 
      immunohistochemistry panel, simultaneously detecting cDC1s, cDC2s, and pDCs 
      within their tissue context. We report on this panel's development, validation, 
      and quantitative analysis. A multiplex immunohistochemistry panel consisting of 
      CD1c, CD303, X-C motif chemokine receptor 1, CD14, CD19, a tumor marker, and DAPI 
      was established. The ImmuNet machine learning pipeline was trained for the 
      detection of DC subsets. The performance of ImmuNet was compared with 
      conventional cell phenotyping software. Ultimately, frequencies of DC subsets 
      within several tumors were defined. In conclusion, this panel provides a method 
      to study cDC1s, cDC2s, and pDCs in the spatial context of the TME, which supports 
      unraveling their specific roles in antitumor immunity.
CI  - (c) 2023 The Authors. European Journal of Immunology published by Wiley-VCH GmbH.
FAU - van der Hoorn, Iris A E
AU  - van der Hoorn IAE
AUID- ORCID: 0009-0008-8644-0678
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
AD  - Department of Pulmonary Diseases, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
FAU - Martynova, Evgenia
AU  - Martynova E
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
AD  - Data Science, Institute for Computing and Information Sciences, Radboud 
      University, Nijmegen, the Netherlands.
FAU - Subtil, Beatriz
AU  - Subtil B
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
FAU - Meek, Jelena
AU  - Meek J
AUID- ORCID: 0000-0001-8439-282X
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
FAU - Verrijp, Kiek
AU  - Verrijp K
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
AD  - Division of Immunotherapy, Oncode Institute, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
AD  - Department of Pathology, Radboud University Medical Center, Nijmegen, the 
      Netherlands.
FAU - Textor, Johannes
AU  - Textor J
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
AD  - Data Science, Institute for Computing and Information Sciences, Radboud 
      University, Nijmegen, the Netherlands.
FAU - Florez-Grau, Georgina
AU  - Florez-Grau G
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
FAU - Piet, Berber
AU  - Piet B
AD  - Department of Pulmonary Diseases, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
FAU - van den Heuvel, Michel M
AU  - van den Heuvel MM
AD  - Department of Pulmonary Diseases, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
FAU - de Vries, I Jolanda M
AU  - de Vries IJM
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
FAU - Gorris, Mark A J
AU  - Gorris MAJ
AD  - Department of Medical BioSciences, Radboud University Medical Center, Nijmegen, 
      the Netherlands.
AD  - Division of Immunotherapy, Oncode Institute, Radboud University Medical Center, 
      Nijmegen, the Netherlands.
LA  - eng
GR  - Hanarth Fonds/
GR  - Vi.Vidi.192.084/Nederlandse Organisatie voor Wetenschappelijk Onderzoek/
GR  - LSHM18056-SGF/Health~Holland/
PT  - Journal Article
DEP - 20231024
PL  - Germany
TA  - Eur J Immunol
JT  - European journal of immunology
JID - 1273201
RN  - 0 (Biomarkers, Tumor)
SB  - IM
MH  - Humans
MH  - *Tumor Microenvironment
MH  - Immunohistochemistry
MH  - Biomarkers, Tumor
MH  - *Neoplasms/metabolism
MH  - Dendritic Cells
OTO - NOTNLM
OT  - DCs
OT  - Multiplex Immunohistochemistry
OT  - Tumor Microenvironment
EDAT- 2023/10/16 06:48
MHDA- 2024/01/30 12:42
CRDT- 2023/10/16 00:13
PHST- 2023/10/12 00:00 [revised]
PHST- 2023/06/19 00:00 [received]
PHST- 2023/10/13 00:00 [accepted]
PHST- 2024/01/30 12:42 [medline]
PHST- 2023/10/16 06:48 [pubmed]
PHST- 2023/10/16 00:13 [entrez]
AID - 10.1002/eji.202350616 [doi]
PST - ppublish
SO  - Eur J Immunol. 2024 Jan;54(1):e2350616. doi: 10.1002/eji.202350616. Epub 2023 Oct 
      24.