PMID- 37840733
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231020
IS  - 1664-302X (Print)
IS  - 1664-302X (Electronic)
IS  - 1664-302X (Linking)
VI  - 14
DP  - 2023
TI  - Fecal microbiota transplantation from HUC-MSC-treated mice alleviates acute lung 
      injury in mice through anti-inflammation and gut microbiota modulation.
PG  - 1243102
LID - 10.3389/fmicb.2023.1243102 [doi]
LID - 1243102
AB  - INTRODUCTION: Acute lung injury (ALI) is a severe respiratory tract disorder 
      facilitated by dysregulated inflammation, oxidative stress and intestinal 
      ecosystem. Fecal microbiota transplantation (FMT) is a rapid method for gut 
      microbiota (GM) reconstruction. Furthermore, our previous studies have confirmed 
      that human umbilical cord mesenchymal stromal cells (HUC-MSCs) can alleviate ALI 
      by improving GM composition. Therefore, we aimed to explore the efficacy and 
      mechanism of FMT from HUC-MSCs-treated mice on ALI. METHODS: In brief, fresh 
      feces from HUC-MSCs-treated mice were collected for FMT, and the mice were 
      randomly assigned into NC, FMT, LPS, ABX-LPS, and ABX-LPS-FMT groups (n = 
      12/group). Subsequently, the mice were administrated with antibiotic mixtures to 
      deplete GM, and given lipopolysaccharide and FMT to induce ALI and rebuild GM. 
      Next, the therapeutic effect was evaluated by bronchoalveolar lavage fluid (BALF) 
      and histopathology. Immune cells in peripheral blood and apoptosis in lung 
      tissues were measured. Furthermore, oxidative stress- and inflammation-related 
      parameter levels were tested in BALF, serum, lung and ileal tissues. The 
      expressions of apoptosis-associated, TLR4/NF-kappaB pathway-associated, Nrf2/HO-1 
      pathway related and tightly linked proteins in the lung and ileal tissues were 
      assessed. Moreover, 16S rRNA was conducted to assess GM composition and 
      distribution. RESULTS: Our results revealed that FMT obviously improved the 
      pathological damage of lung and ileum, recovered the immune system of peripheral 
      blood, decreased the cell apoptosis of lung, and inhibited inflammation and 
      oxidative stress in BALF, serum, lung and ileum tissues. Moreover, FMT also 
      elevated ZO-1, claudin-1, and occludin protein expressions, activating the 
      Nrf2/HO-1 pathway but hindering the TLR4/NF-kappaB pathway. Of note, the relative 
      abundances of Bacteroides, Christensenella, Coprococcus, and Roseburia were 
      decreased, while the relative abundances of Xenorhabdus, Sutterella, and 
      Acinetobacter were increased in the ABX-LPS-FMT group. CONCLUSION: FMT from 
      HUC-MSCs-treated mice may alleviate ALI by inhibiting inflammation and 
      reconstructing GM, additionally, we also found that the TLR4/NF-kappaB and Nrf2/HO-1 
      pathways may involve in the improvement of FMT on ALI, which offers novel 
      insights for the functions and mechanisms of FMT from HUC-MSCs-treated mice on 
      ALI.
CI  - Copyright (c) 2023 Hua, Cui, Lv, Wang, Li, Lu, Chen and Chen.
FAU - Hua, Feng
AU  - Hua F
AD  - Department of Respiratory and Critical Care Medicine, Huzhou Central Hospital, 
      Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, 
      China.
FAU - Cui, Enhai
AU  - Cui E
AD  - Department of Respiratory and Critical Care Medicine, Huzhou Central Hospital, 
      Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, 
      China.
FAU - Lv, Lu
AU  - Lv L
AD  - Department of Respiratory and Critical Care Medicine, Huzhou Central Hospital, 
      Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, 
      China.
FAU - Wang, Bin
AU  - Wang B
AD  - Department of Respiratory and Critical Care Medicine, Huzhou Central Hospital, 
      Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, 
      China.
FAU - Li, Liqin
AU  - Li L
AD  - Traditional Chinese Medicine Key Laboratory Cultivation Base of Zhejiang Province 
      for the Development and Clinical Transformation of Immunomodulatory Drugs, 
      Huzhou, China.
FAU - Lu, Huadong
AU  - Lu H
AD  - Department of Respiratory and Critical Care Medicine, Huzhou Central Hospital, 
      Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, 
      China.
FAU - Chen, Na
AU  - Chen N
AD  - Department of Respiratory and Critical Care Medicine, Huzhou Central Hospital, 
      Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, 
      China.
FAU - Chen, Wenyan
AU  - Chen W
AD  - Department of Respiratory and Critical Care Medicine, Huzhou Central Hospital, 
      Affiliated Huzhou Hospital, Zhejiang University School of Medicine, Huzhou, 
      China.
LA  - eng
PT  - Journal Article
DEP - 20230928
PL  - Switzerland
TA  - Front Microbiol
JT  - Frontiers in microbiology
JID - 101548977
PMC - PMC10569429
OTO - NOTNLM
OT  - acute lung injury
OT  - fecal microbiota transplantation
OT  - gut microbiota
OT  - human umbilical cord mesenchymal stromal cells
OT  - inflammation
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/10/16 06:48
MHDA- 2023/10/16 06:49
PMCR- 2023/09/28
CRDT- 2023/10/16 04:20
PHST- 2023/06/20 00:00 [received]
PHST- 2023/08/29 00:00 [accepted]
PHST- 2023/10/16 06:49 [medline]
PHST- 2023/10/16 06:48 [pubmed]
PHST- 2023/10/16 04:20 [entrez]
PHST- 2023/09/28 00:00 [pmc-release]
AID - 10.3389/fmicb.2023.1243102 [doi]
PST - epublish
SO  - Front Microbiol. 2023 Sep 28;14:1243102. doi: 10.3389/fmicb.2023.1243102. 
      eCollection 2023.