PMID- 37841648
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231020
IS  - 2405-6383 (Electronic)
IS  - 2405-6545 (Print)
IS  - 2405-6383 (Linking)
VI  - 15
DP  - 2023 Dec
TI  - Propionate promotes gluconeogenesis by regulating mechanistic target of rapamycin 
      (mTOR) pathway in calf hepatocytes.
PG  - 88-98
LID - 10.1016/j.aninu.2023.07.001 [doi]
AB  - Enhancing hepatic gluconeogenesis is one of the main modes of meeting the glucose 
      requirement of dairy cows. This study attempted to determine whether the 
      gluconeogenesis precursor propionate had an effect on the expression of the main 
      genes involved in gluconeogenesis in calf hepatocytes and elucidate the 
      associated mechanisms. Calf hepatocytes were obtained from 5 healthy calves (1 d 
      old; 30 to 40 kg) and exposed to 0-, 1-, 2.5-, or 5-mM sodium propionate (NaP), 
      which is known to promote the expression of genes involved in the gluconeogenesis 
      pathway, including fructose 1,6-bisphosphatase, phosphoenolpyruvate 
      carboxykinase, and glucose-6-phosphatase. With regard to the underlying 
      mechanism, propionate promoted the expression of peroxisome 
      proliferator-activated receptor gamma coactivator 1-alpha, hepatocyte nuclear 
      factor 4, and forkhead box O1 (transcription factors that regulate the expression 
      of hepatic gluconeogenic genes) by promoting mammalian target of rapamycin 
      complex 1 (mTORC1), but inhibiting mTORC2 activity (P < 0.01). We also 
      established a model of palmitic acid (PA)-induced hepatic injury in calf 
      hepatocytes and found that PA could inhibit the gluconeogenic capacity of calf 
      hepatocytes by suppressing the expression of gluconeogenic genes, inhibiting 
      mTORC1, and promoting the activity of mTORC2 (P < 0.01). In contrast, NaP 
      provided protection to calf hepatocytes by counteracting the inhibitory effect of 
      PA on the gluconeogenic capacity of calf hepatocytes (P < 0.05). Collectively, 
      these findings indicate that NaP enhances the gluconeogenic capacity of calf 
      hepatocytes by regulating the mTOR pathway activity. Thus, in addition to 
      improving the glucose production potential, propionate may have therapeutic 
      potential for the treatment of hepatic injury in dairy cows.
CI  - (c) 2023 The Authors. Publishing services by Elsevier B.V. on behalf of KeAi 
      Communications Co. Ltd.
FAU - Wang, Guo Yan
AU  - Wang GY
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Qin, Sen Lin
AU  - Qin SL
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Zheng, Yi Ning
AU  - Zheng YN
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Geng, Hui Jun
AU  - Geng HJ
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Chen, Lei
AU  - Chen L
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Yao, Jun Hu
AU  - Yao JH
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
FAU - Deng, Lu
AU  - Deng L
AD  - College of Animal Science and Technology, Northwest A&F University, Yangling, 
      Shaanxi 712100, China.
LA  - eng
PT  - Journal Article
DEP - 20230722
PL  - China
TA  - Anim Nutr
JT  - Animal nutrition (Zhongguo xu mu shou yi xue hui)
JID - 101717655
PMC - PMC10568569
OTO - NOTNLM
OT  - Gluconeogenesis
OT  - Mechanistic target of rapamycin
OT  - Palmitic acid
OT  - Propionate
COIS- We declare that we have no financial and personal relationships with other people 
      or organizations that can inappropriately influence our work, and there is no 
      professional or other personal interest of any nature or kind in any product, 
      service and/or company that could be construed as influencing the content of this 
      paper.
EDAT- 2023/10/16 06:48
MHDA- 2023/10/16 06:49
PMCR- 2023/07/22
CRDT- 2023/10/16 04:38
PHST- 2022/11/01 00:00 [received]
PHST- 2023/06/13 00:00 [revised]
PHST- 2023/07/18 00:00 [accepted]
PHST- 2023/10/16 06:49 [medline]
PHST- 2023/10/16 06:48 [pubmed]
PHST- 2023/10/16 04:38 [entrez]
PHST- 2023/07/22 00:00 [pmc-release]
AID - S2405-6545(23)00090-2 [pii]
AID - 10.1016/j.aninu.2023.07.001 [doi]
PST - epublish
SO  - Anim Nutr. 2023 Jul 22;15:88-98. doi: 10.1016/j.aninu.2023.07.001. eCollection 
      2023 Dec.