PMID- 37842917
OWN - NLM
STAT- MEDLINE
DCOM- 20231114
LR  - 20231118
IS  - 1526-4602 (Electronic)
IS  - 1525-7797 (Print)
IS  - 1525-7797 (Linking)
VI  - 24
IP  - 11
DP  - 2023 Nov 13
TI  - Membrane-Active Thermoresponsive Block Copolymers Containing a 
      Diacylglycerol-Based Segment: RAFT Synthesis, Doxorubicin Encapsulation, and 
      Evaluation of Cytotoxicity against Breast Cancer Cells.
PG  - 4854-4868
LID - 10.1021/acs.biomac.3c00580 [doi]
AB  - Herein, we report the formation of drug delivery systems from original 
      thermoresponsive block copolymers containing lipid-based segments. Two acrylate 
      monomers derived from palmitic- or oleic-acid-based diacylglycerols (DAGs) were 
      synthesized and polymerized by the reversible addition-fragmentation chain 
      transfer (RAFT) method. Well-defined DAG-based polymers with targeted molar 
      masses and narrow molar mass distributions were next used as macro-chain transfer 
      agents (macro-CTAs) for the polymerization of N-isopropylacrylamide (NIPAAm) or 
      N-vinylcaprolactam (NVCL). The obtained amphiphilic block copolymers were formed 
      into polymeric nanoparticles (PNPs) with and without encapsulated doxorubicin and 
      characterized. Their biological assessment indicated appropriate 
      cytocompatibility with the representatives of normal cells. Furthermore, compared 
      to the free drug, increased cytotoxicity and apoptosis or necrosis induction in 
      breast cancer cells was documented, including a highly aggressive and invasive 
      triple-negative MDA-MB-231 cell line.
FAU - Kurowska, Izabela
AU  - Kurowska I
AD  - Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, Bialystok 
      15-245, Poland.
AD  - Doctoral School of Exact and Natural Sciences, University of Bialystok, Bialystok 
      15-245, Poland.
FAU - Markiewicz, Karolina H
AU  - Markiewicz KH
AUID- ORCID: 0000-0002-4857-0489
AD  - Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, Bialystok 
      15-245, Poland.
FAU - Niemirowicz-Laskowska, Katarzyna
AU  - Niemirowicz-Laskowska K
AD  - Department of Experimental Pharmacology, Medical University of Bialystok, 
      Szpitalna 37, Bialystok 15-295, Poland.
FAU - Destarac, Mathias
AU  - Destarac M
AUID- ORCID: 0000-0002-9718-2239
AD  - Laboratoire IMRCP, CNRS UMR 5623, Paul Sabatier University, Toulouse Cedex 09 
      31062, France.
FAU - Wielgat, Przemyslaw
AU  - Wielgat P
AD  - Department of Clinical Pharmacology, Medical University of Bialystok, Waszyngtona 
      15A, Bialystok 15-274, Poland.
FAU - Misztalewska-Turkowicz, Iwona
AU  - Misztalewska-Turkowicz I
AUID- ORCID: 0000-0001-6191-6053
AD  - Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, Bialystok 
      15-245, Poland.
FAU - Misiak, Pawel
AU  - Misiak P
AUID- ORCID: 0000-0002-6882-3519
AD  - Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, Bialystok 
      15-245, Poland.
FAU - Car, Halina
AU  - Car H
AD  - Department of Experimental Pharmacology, Medical University of Bialystok, 
      Szpitalna 37, Bialystok 15-295, Poland.
AD  - Department of Clinical Pharmacology, Medical University of Bialystok, Waszyngtona 
      15A, Bialystok 15-274, Poland.
FAU - Wilczewska, Agnieszka Z
AU  - Wilczewska AZ
AUID- ORCID: 0000-0001-8587-6711
AD  - Faculty of Chemistry, University of Bialystok, Ciolkowskiego 1K, Bialystok 
      15-245, Poland.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231016
PL  - United States
TA  - Biomacromolecules
JT  - Biomacromolecules
JID - 100892849
RN  - 0 (Diglycerides)
RN  - 80168379AG (Doxorubicin)
RN  - 0 (Polymers)
SB  - IM
MH  - Humans
MH  - Female
MH  - *Breast Neoplasms/drug therapy
MH  - Diglycerides
MH  - Doxorubicin/pharmacology
MH  - Polymers/pharmacology
MH  - Drug Delivery Systems/methods
PMC - PMC10646981
COIS- The authors declare no competing financial interest.
EDAT- 2023/10/16 12:42
MHDA- 2023/11/14 06:42
PMCR- 2023/11/15
CRDT- 2023/10/16 06:53
PHST- 2023/11/14 06:42 [medline]
PHST- 2023/10/16 12:42 [pubmed]
PHST- 2023/10/16 06:53 [entrez]
PHST- 2023/11/15 00:00 [pmc-release]
AID - 10.1021/acs.biomac.3c00580 [doi]
PST - ppublish
SO  - Biomacromolecules. 2023 Nov 13;24(11):4854-4868. doi: 10.1021/acs.biomac.3c00580. 
      Epub 2023 Oct 16.