PMID- 37844852
OWN - NLM
STAT- MEDLINE
DCOM- 20231103
LR  - 20231103
IS  - 1095-9947 (Electronic)
IS  - 1050-4648 (Linking)
VI  - 142
DP  - 2023 Nov
TI  - Antiviral role of grouper FoxO1 against RGNNV and SGIV infection.
PG  - 109168
LID - S1050-4648(23)00654-X [pii]
LID - 10.1016/j.fsi.2023.109168 [doi]
AB  - As a key regulator of the innate immune system, FoxO1 has a variety of activities 
      in biological organisms. In the present study, grouper FoxO1 (EcFoxO1) was cloned 
      and the antiviral activity in red grouper neuron necrosis virus (RGNNV) and 
      Singapore grouper iridescent virus (SGIV) was examined. The open reading frame 
      (ORF) of EcFoxO1 contains 2,034 base pairs that encode a protein of 677 amino 
      acids with a predicted molecular weight of 73.21 kDa. EcFoxO1 was shown to be 
      broadly distributed in healthy grouper tissues, and was up-regulated in vitro in 
      response to stimulation by RGNNV and SGIV. EcFoxO1 has a whole-cell distribution 
      in grouper spleen (GS) cells. EcFoxO1 decreased the replication of RGNNV and 
      SGIV, and activated interferon (IFN) 3, IFN-stimulated response element (ISRE), 
      and nuclear factor-kappaB (NF-kappaB) promoter activities. EcFoxO1 could interact with 
      EcIRF3. Together, the results demonstrated that EcFoxO1 might be an important 
      regulator of grouper innate immune response against RGNNV and SGIV infection.
CI  - Copyright (c) 2023. Published by Elsevier Ltd.
FAU - Liu, Shaoli
AU  - Liu S
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Lei, Xiaoxia
AU  - Lei X
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Cao, Helong
AU  - Cao H
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Xu, Zhuqing
AU  - Xu Z
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Wu, Siting
AU  - Wu S
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Chen, Hong
AU  - Chen H
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Xu, Linting
AU  - Xu L
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Zhan, Zhouling
AU  - Zhan Z
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Xu, Qiongyue
AU  - Xu Q
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China.
FAU - Wei, Jingguang
AU  - Wei J
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China. Electronic address: weijg@scau.edu.cn.
FAU - Qin, Qiwei
AU  - Qin Q
AD  - College of Marine Sciences, South China Agricultural University, Guangdong 
      Laboratory for Lingnan Modern Agriculture, Guangzhou, 510642, China; Nansha-South 
      China Agricultural University Fishery Research Institute,Guangzhou, Guangzhou, 
      511400,China; Southern Marine Science and Engineering Guangdong Laboratory 
      (Zhuhai), Zhuhai, China. Electronic address: qinqw@scau.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231014
PL  - England
TA  - Fish Shellfish Immunol
JT  - Fish & shellfish immunology
JID - 9505220
RN  - 0 (Fish Proteins)
RN  - 0 (Antiviral Agents)
SB  - IM
MH  - Animals
MH  - *Bass
MH  - Gene Expression Regulation
MH  - Fish Proteins/chemistry
MH  - Amino Acid Sequence
MH  - *Ranavirus/physiology
MH  - Immunity, Innate/genetics
MH  - Antiviral Agents
MH  - Neurons
MH  - *Fish Diseases
MH  - *DNA Virus Infections
OTO - NOTNLM
OT  - FoxO1
OT  - Grouper
OT  - Interferon
OT  - RGNNV
OT  - SGIV
COIS- Declaration of competing interest The authors declare no conflict of interest.
EDAT- 2023/10/17 00:42
MHDA- 2023/11/03 06:44
CRDT- 2023/10/16 19:25
PHST- 2023/09/29 00:00 [received]
PHST- 2023/10/11 00:00 [revised]
PHST- 2023/10/14 00:00 [accepted]
PHST- 2023/11/03 06:44 [medline]
PHST- 2023/10/17 00:42 [pubmed]
PHST- 2023/10/16 19:25 [entrez]
AID - S1050-4648(23)00654-X [pii]
AID - 10.1016/j.fsi.2023.109168 [doi]
PST - ppublish
SO  - Fish Shellfish Immunol. 2023 Nov;142:109168. doi: 10.1016/j.fsi.2023.109168. Epub 
      2023 Oct 14.