PMID- 37849216
OWN - NLM
STAT- MEDLINE
DCOM- 20231129
LR  - 20231129
IS  - 1600-0722 (Electronic)
IS  - 0909-8836 (Linking)
VI  - 131
IP  - 5-6
DP  - 2023 Oct-Nov
TI  - Role of alpha smooth muscle actin in odontogenic differentiation of dental pulp 
      stem cells.
PG  - e12956
LID - 10.1111/eos.12956 [doi]
AB  - Pulpotomy is an effective treatment for retaining vital pulp after pulp exposure 
      caused by caries removal and/or trauma. The expression of alpha smooth muscle 
      actin (alpha-SMA) is increased during the wound-healing process, and alpha-SMA-positive 
      fibroblasts accelerate tissue repair. However, it remains largely unknown whether 
      alpha-SMA-positive fibroblasts influence pulpal repair. In this study, we established 
      an experimental rat pulpotomy model and found that the expression of alpha-SMA was 
      increased in dental pulp after pulpotomy relative to that in normal dental pulp. 
      In vitro results showed that the expression of alpha-SMA was increased during the 
      induction of odontogenic differentiation in dental pulp stem cells (DPSCs) 
      compared with untreated DPSCs. Moreover, alpha-SMA overexpression promoted the 
      odontogenic differentiation of DPSCs via increasing mitochondrial function. 
      Mechanistically, alpha-SMA overexpression activated the mammalian target of rapamycin 
      (mTOR) signaling pathway. Inhibition of the mTOR signaling pathway by rapamycin 
      decreased the mitochondrial function in alpha-SMA-overexpressing DPSCs and suppressed 
      the odontogenic differentiation of DPSCs. Furthermore, we found that alpha-SMA 
      overexpression increased the secretion of transforming growth factor beta-1 
      (TGF-beta1). In sum, our present study demonstrates a novel mechanism by which alpha-SMA 
      promotes odontogenic differentiation of DPSCs by increasing mitochondrial 
      respiratory activity via the mTOR signaling pathway.
CI  - (c) 2023 Scandinavian Division of the International Association for Dental 
      Research. Published by John Wiley & Sons Ltd.
FAU - Ma, Zeyi
AU  - Ma Z
AUID- ORCID: 0000-0002-8598-535X
AD  - Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.
FAU - Shen, Peiqi
AU  - Shen P
AUID- ORCID: 0000-0003-2583-8574
AD  - Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.
FAU - Xu, Xiaoqing
AU  - Xu X
AUID- ORCID: 0000-0002-3364-1344
AD  - Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.
FAU - Li, Weiyu
AU  - Li W
AUID- ORCID: 0000-0002-8964-6232
AD  - Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.
FAU - Li, Yaoyin
AU  - Li Y
AUID- ORCID: 0000-0002-2360-7847
AD  - Hospital of Stomatology, Guanghua School of Stomatology, Sun Yat-sen University, 
      Guangzhou, Guangdong, China.
AD  - Guangdong Provincial Key Laboratory of Stomatology, Guangzhou, Guangdong, China.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231017
PL  - England
TA  - Eur J Oral Sci
JT  - European journal of oral sciences
JID - 9504563
RN  - 0 (Acta2 protein, rat)
RN  - 0 (Actins)
RN  - EC 2.7.11.1 (TOR Serine-Threonine Kinases)
SB  - IM
MH  - Animals
MH  - Rats
MH  - *Actins/metabolism/pharmacology
MH  - Cell Differentiation
MH  - Cell Proliferation
MH  - Cells, Cultured
MH  - *Dental Pulp/cytology/growth & development
MH  - *Odontogenesis
MH  - Stem Cells
MH  - TOR Serine-Threonine Kinases/metabolism/pharmacology
MH  - Pulpotomy
OTO - NOTNLM
OT  - cell differentiation
OT  - dentinogenesis
OT  - mitochondria
OT  - stem cells
EDAT- 2023/10/18 06:44
MHDA- 2023/11/06 06:41
CRDT- 2023/10/18 01:12
PHST- 2023/04/17 00:00 [received]
PHST- 2023/09/20 00:00 [accepted]
PHST- 2023/11/06 06:41 [medline]
PHST- 2023/10/18 06:44 [pubmed]
PHST- 2023/10/18 01:12 [entrez]
AID - 10.1111/eos.12956 [doi]
PST - ppublish
SO  - Eur J Oral Sci. 2023 Oct-Nov;131(5-6):e12956. doi: 10.1111/eos.12956. Epub 2023 
      Oct 17.