PMID- 37852047 OWN - NLM STAT- MEDLINE DCOM- 20231106 LR - 20231106 IS - 1873-3778 (Electronic) IS - 0021-9673 (Linking) VI - 1711 DP - 2023 Nov 22 TI - Simultaneous determination of three biomarkers of non-small cells lung cancer in urine by pipette-tip solid-phase extraction coupled with liquid chromatography tandem mass spectrometry. PG - 464448 LID - S0021-9673(23)00673-8 [pii] LID - 10.1016/j.chroma.2023.464448 [doi] AB - Simultaneous determination of multiple biomarkers can improve the effectiveness and accuracy of cancer diagnosis. Cortisol, cortisone, and 4-methoxyphenylacetic acid (4-Me) are metabolic biomarker group with high specificity and sensitivity for the diagnosis of non-small cells lung cancer (NSCLC), and the development of their simultaneous determination method is desired. Herein, a simple, sensitive, and low-cost method involving pipette-tip solid-phase extraction (PT-SPE) using anion exchange adsorbent (MAX) coupled with liquid chromatography tandem mass spectrometry (LC-MS/MS) was developed for the simultaneous determination of three biomarkers (cortisol, cortisone, and 4-Me) in human urine. The sample (0.1 mL), adsorbent (1.5 mg) and organic reagent (3.5 mL) of MAX-PT-SPE are less consumed, and have the advantages of easy access to raw materials, simple assembly, convenient on-site instant extraction, low pollution, and low cost. The limits of detection of the three biomarkers were 0.006-0.024 ng mL(-1), the recoveries of three spiked levels (2, 50, and 500 ng mL(-1)) were 91.0%-99.3%, with the relative standard deviations (RSDs)