PMID- 37852624
OWN - NLM
STAT- MEDLINE
DCOM- 20240214
LR  - 20240214
IS  - 1600-0536 (Electronic)
IS  - 0105-1873 (Linking)
VI  - 90
IP  - 3
DP  - 2024 Mar
TI  - The BMDC model, a performant cell-based test to assess the sensitizing potential 
      and potency of chemicals including pre/pro-haptens.
PG  - 211-234
LID - 10.1111/cod.14439 [doi]
AB  - BACKGROUND: Chemical-induced allergies at workplace represent a significant 
      occupational health issue. These substances must be properly identified as 
      sensitizers. In previous studies, an original model using mouse bone 
      marrow-derived dendritic cells (BMDC) was developed for this purpose. OBJECTIVES: 
      The aim of this study was to evaluate the predictive capacity of the BMDC model 
      with a large panel of sensitizers (including pre- and pro-haptens) and 
      non-sensitizers. METHODS: The readout from the BMDC model is based on expression 
      levels of six phenotypic markers measured by flow cytometry. RESULTS: The results 
      indicate that 29 of the 37 non-sensitizers, and 81 of the 86 sensitizers were 
      correctly classified compared to the Local Lymph Node Assay (LLNA). Statistical 
      analysis revealed the BMDC model to have a sensitivity of 94%, a specificity of 
      78%, and an accuracy of 89%. The EC2 (Effective Concentration) values calculated 
      with this model allow sensitizers to be categorized into four classes: extreme, 
      strong, moderate and weak. CONCLUSIONS: These excellent predictive performances 
      show that the BMDC model discriminates between sensitizers and non-sensitizers 
      with outstanding precision equal to or better than existing validated alternative 
      models. Moreover, this model allows to predict sensitization potency of 
      chemicals. The BMDC test could therefore be proposed as an additional tool to 
      assess the sensitizing potential and potency of chemicals.
CI  - (c) 2023 The Authors. Contact Dermatitis published by John Wiley & Sons Ltd.
FAU - Battais, Fabrice
AU  - Battais F
AUID- ORCID: 0000-0002-1393-1686
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Langonne, Isabelle
AU  - Langonne I
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Muller, Samuel
AU  - Muller S
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Mathiot, Julianne
AU  - Mathiot J
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Coiscaud, Amelie
AU  - Coiscaud A
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Audry, Adrien
AU  - Audry A
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Remy, Aurelie Martin
AU  - Remy AM
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Sponne, Isabelle
AU  - Sponne I
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
FAU - Mourot-Bousquenaud, Melanie
AU  - Mourot-Bousquenaud M
AUID- ORCID: 0000-0003-0556-0798
AD  - French Research and Safety Institute for the Prevention of Occupational Accidents 
      and Diseases (INRS), Toxicology and Biomonitoring Division, Vandoeuvre les Nancy, 
      France.
LA  - eng
PT  - Journal Article
DEP - 20231018
PL  - England
TA  - Contact Dermatitis
JT  - Contact dermatitis
JID - 7604950
RN  - 0 (Haptens)
RN  - 0 (Allergens)
SB  - IM
MH  - Mice
MH  - Animals
MH  - *Dermatitis, Allergic Contact/diagnosis/etiology
MH  - Haptens
MH  - Local Lymph Node Assay
MH  - Flow Cytometry
MH  - Allergens/adverse effects
OTO - NOTNLM
OT  - bone marrow-derived dendritic cells
OT  - chemical allergens
OT  - in vitro assay
OT  - potency
OT  - sensitization
EDAT- 2023/10/19 00:44
MHDA- 2024/02/12 18:42
CRDT- 2023/10/18 20:22
PHST- 2023/09/07 00:00 [revised]
PHST- 2023/06/02 00:00 [received]
PHST- 2023/10/01 00:00 [accepted]
PHST- 2024/02/12 18:42 [medline]
PHST- 2023/10/19 00:44 [pubmed]
PHST- 2023/10/18 20:22 [entrez]
AID - 10.1111/cod.14439 [doi]
PST - ppublish
SO  - Contact Dermatitis. 2024 Mar;90(3):211-234. doi: 10.1111/cod.14439. Epub 2023 Oct 
      18.