PMID- 37858141
OWN - NLM
STAT- MEDLINE
DCOM- 20231023
LR  - 20231119
IS  - 1746-6148 (Electronic)
IS  - 1746-6148 (Linking)
VI  - 19
IP  - 1
DP  - 2023 Oct 20
TI  - Levels of neutralizing antibodies against resident farm strain or vaccine strain 
      are not indicators of protection against PRRSV-1 vertical transmission under farm 
      conditions.
PG  - 217
LID - 10.1186/s12917-023-03785-z [doi]
LID - 217
AB  - BACKGROUND: Vertical transmission is key for the maintenance of porcine 
      reproductive and respiratory syndrome virus (PRRSV) infection. In vaccinated 
      farms, vertical transmission can still occur despite sows having some level of 
      immunity because of repeated vaccination or contact with the wild-type virus. The 
      present study aimed to correlate the age of sows and the amplitude of 
      neutralizing antibodies (Nab) (heterologous neutralization) with PRRSV-1 vertical 
      transmission (VT). For this purpose, umbilical cords of 1,554 newborns 
      (corresponding to 250 litters) were tested for PRRSV by RT-PCR in two 
      PRRSV-unstable vaccinated farms. In parallel, the sows were bled after farrowing 
      and the levels of antibodies were determined by ELISA and by the viral 
      neutralization test against the vaccine virus, the virus circulating in the farm, 
      and other unrelated contemporary PRRSV-1 strains. The relationship between the 
      parity and the probability of delivering infected piglets and the presence of 
      broadly Nabs examined. RESULTS: The proportion of VT events in the two examined 
      farms ranged from 18.9% to 23.0%. Young sows (parity 1-2) were 1.7 times more 
      likely to have VT than older sows (p < 0.05). Despite higher ELISA S/P antibody 
      ratios in younger sows (p < 0.05), NAb against the resident farm strain were at a 
      similar level between sows delivering infected and healthy piglets regardless of 
      age, mostly with low titers (2-3 log(2)). The titers of NAb against the vaccine 
      virus were also low, and no correlations with VT were observed. When a panel of 
      another 4 strains (1 isolated in the 1990s, and 3 contemporary strains) were used 
      for the neutralization test, most sow sera were not capable of neutralizing the 
      contemporary strains. CONCLUSIONS: Titers of NAb could not be correlated with the 
      occurrence of PRRSV VT. The amplitude of NAb present in most vaccinated sows is 
      limited with a considerable proportion unresponsive regarding NAb production.
CI  - (c) 2023. BioMed Central Ltd., part of Springer Nature.
FAU - Martin-Valls, Gerard Eduard
AU  - Martin-Valls GE
AD  - Departament de Sanitat i Anatomia Animals, Facultat de Veterinaria, Travessera 
      dels Turons S/N, Universitat Autonoma de Barcelona, 08193, Cerdanyola del Valles, 
      Spain.
FAU - Li, Yanli
AU  - Li Y
AD  - Departament de Sanitat i Anatomia Animals, Facultat de Veterinaria, Travessera 
      dels Turons S/N, Universitat Autonoma de Barcelona, 08193, Cerdanyola del Valles, 
      Spain.
FAU - Clilverd, Hepzibar
AU  - Clilverd H
AD  - Departament de Sanitat i Anatomia Animals, Facultat de Veterinaria, Travessera 
      dels Turons S/N, Universitat Autonoma de Barcelona, 08193, Cerdanyola del Valles, 
      Spain.
FAU - Soto, Jordi
AU  - Soto J
AD  - Departament de Sanitat i Anatomia Animals, Facultat de Veterinaria, Travessera 
      dels Turons S/N, Universitat Autonoma de Barcelona, 08193, Cerdanyola del Valles, 
      Spain.
FAU - Cortey, Marti
AU  - Cortey M
AD  - Departament de Sanitat i Anatomia Animals, Facultat de Veterinaria, Travessera 
      dels Turons S/N, Universitat Autonoma de Barcelona, 08193, Cerdanyola del Valles, 
      Spain.
FAU - Mateu, Enric
AU  - Mateu E
AD  - Departament de Sanitat i Anatomia Animals, Facultat de Veterinaria, Travessera 
      dels Turons S/N, Universitat Autonoma de Barcelona, 08193, Cerdanyola del Valles, 
      Spain. enric.mateu@uab.cat.
LA  - eng
GR  - Own funds/Laboratori Veterinari de Diagnosi de Malalties Infeccioses/
GR  - Boehringer Ingelheim European PRRS Research Award/Boehringer Ingelheim/
PT  - Journal Article
DEP - 20231020
PL  - England
TA  - BMC Vet Res
JT  - BMC veterinary research
JID - 101249759
RN  - 0 (Antibodies, Neutralizing)
RN  - 0 (Antibodies, Viral)
RN  - 0 (Viral Vaccines)
SB  - IM
MH  - Pregnancy
MH  - Animals
MH  - Swine
MH  - Female
MH  - *Porcine respiratory and reproductive syndrome virus
MH  - Antibodies, Neutralizing
MH  - *Porcine Reproductive and Respiratory Syndrome/prevention & control
MH  - Farms
MH  - Antibodies, Viral
MH  - *Viral Vaccines
MH  - *Swine Diseases/prevention & control
PMC - PMC10588270
OTO - NOTNLM
OT  - Broadly neutralizing antibodies
OT  - Neutralizing antibodies
OT  - Porcine reproductive and respiratory virus
OT  - Vertical transmission
COIS- EM has carried out several research contracts with companies in the 
      pharmaceutical sector that manufacture vaccines against PRRSV. None of them 
      participated in this study nor have they participated in the design of the study, 
      selection of farms, taking of samples, obtaining results, data analysis, data 
      interpretation, writing of the document or discussion of the results. All the 
      other authors declare no competing interests.
EDAT- 2023/10/20 00:43
MHDA- 2023/10/23 01:18
PMCR- 2023/10/20
CRDT- 2023/10/20 00:00
PHST- 2023/03/12 00:00 [received]
PHST- 2023/10/10 00:00 [accepted]
PHST- 2023/10/23 01:18 [medline]
PHST- 2023/10/20 00:43 [pubmed]
PHST- 2023/10/20 00:00 [entrez]
PHST- 2023/10/20 00:00 [pmc-release]
AID - 10.1186/s12917-023-03785-z [pii]
AID - 3785 [pii]
AID - 10.1186/s12917-023-03785-z [doi]
PST - epublish
SO  - BMC Vet Res. 2023 Oct 20;19(1):217. doi: 10.1186/s12917-023-03785-z.