PMID- 37858304
OWN - NLM
STAT- MEDLINE
DCOM- 20231113
LR  - 20231129
IS  - 2049-632X (Electronic)
IS  - 2049-632X (Linking)
VI  - 81
DP  - 2023 Jan 17
TI  - Myeloid miR-155 plays a limited role in antibacterial defense during 
      Klebsiella-derived pneumosepsis and is dispensable for lipopolysaccharide- or 
      Klebsiella-induced inflammation in mice.
LID - 10.1093/femspd/ftad031 [doi]
LID - ftad031
AB  - MicroRNA-155 (miR-155) plays a crucial role in regulating host inflammatory 
      responses during bacterial infection. Previous studies have shown that 
      constitutive miR-155 deficiency alleviates inflammation while having varying 
      effects in different bacterial infection models. However, whether miR-155 in 
      myeloid cells is involved in the regulation of inflammatory and antibacterial 
      responses is largely elusive. Mice with myeloid cell specific miR-155 deficiency 
      were generated to study the in vitro response of bone marrow-derived macrophages 
      (BMDMs), alveolar macrophages (AMs) and peritoneal macrophages (PMs) to 
      lipopolysaccharide (LPS), and the in vivo response after intranasal or 
      intraperitoneal challenge with LPS or infection with Klebsiella (K.) pneumoniae 
      via the airways. MiR-155-deficient macrophages released less inflammatory 
      cytokines than control macrophages upon stimulation with LPS in vitro. However, 
      the in vivo inflammatory cytokine response to LPS or K. pneumoniae was not 
      affected by myeloid miR-155 deficiency. Moreover, bacterial outgrowth in the 
      lungs was not altered in myeloid miR-155-deficient mice, but Klebsiella loads in 
      the liver of these mice were significantly higher than in control mice. These 
      data argue against a major role for myeloid miR-155 in host inflammatory 
      responses during LPS-induced inflammation and K. pneumoniae-induced pneumosepsis 
      but suggest that myeloid miR-155 contributes to host defense against Klebsiella 
      infection in the liver.
CI  - (c) The Author(s) 2023. Published by Oxford University Press on behalf of FEMS.
FAU - Qin, Wanhai
AU  - Qin W
AUID- ORCID: 0000-0002-7776-5007
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The 
      Netherlands.
AD  - Amsterdam Infection and Immunity Institute, 1105 AZ Amsterdam, The Netherlands.
FAU - Saris, Anno
AU  - Saris A
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The 
      Netherlands.
AD  - Amsterdam Infection and Immunity Institute, 1105 AZ Amsterdam, The Netherlands.
FAU - van 't Veer, Cornelis
AU  - van 't Veer C
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The 
      Netherlands.
AD  - Amsterdam Infection and Immunity Institute, 1105 AZ Amsterdam, The Netherlands.
FAU - Roelofs, Joris J T H
AU  - Roelofs JJTH
AD  - Department of Pathology, Amsterdam University Medical Centers, Academic Medical 
      Center, University of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
AD  - Amsterdam Cardiovascular Sciences, University of Amsterdam, 1105 AZ Amsterdam, 
      The Netherlands.
FAU - Scicluna, Brendon P
AU  - Scicluna BP
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The 
      Netherlands.
AD  - Amsterdam Infection and Immunity Institute, 1105 AZ Amsterdam, The Netherlands.
AD  - Department of Applied Biomedical Science, Faculty of Health Sciences, Mater Dei 
      Hospital, University of Malta, MSD 2080, Msida, Malta.
AD  - Centre for Molecular Medicine and Biobanking, University of Malta, MSD 2080, 
      Msida, Malta.
FAU - de Vos, Alex F
AU  - de Vos AF
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The 
      Netherlands.
AD  - Amsterdam Infection and Immunity Institute, 1105 AZ Amsterdam, The Netherlands.
FAU - van der Poll, Tom
AU  - van der Poll T
AD  - Center for Experimental and Molecular Medicine, Amsterdam University Medical 
      Centers, Academic Medical Center, University of Amsterdam, 1105 AZ Amsterdam, The 
      Netherlands.
AD  - Amsterdam Infection and Immunity Institute, 1105 AZ Amsterdam, The Netherlands.
AD  - Division of Infectious Diseases, Amsterdam University Medical Centers, University 
      of Amsterdam, 1105 AZ Amsterdam, The Netherlands.
LA  - eng
GR  - #20160617115/China Scholarship Council/
PT  - Journal Article
PL  - United States
TA  - Pathog Dis
JT  - Pathogens and disease
JID - 101595366
RN  - 0 (Lipopolysaccharides)
RN  - 0 (Cytokines)
RN  - 0 (MicroRNAs)
RN  - 0 (Mirn155 microRNA, mouse)
SB  - IM
MH  - Animals
MH  - Mice
MH  - Lipopolysaccharides
MH  - Klebsiella/genetics
MH  - Inflammation
MH  - Klebsiella pneumoniae/physiology
MH  - Cytokines
MH  - *Klebsiella Infections/microbiology
MH  - *MicroRNAs/genetics
MH  - Mice, Inbred C57BL
PMC - PMC10636497
OTO - NOTNLM
OT  - anti-bacterial defense
OT  - inflammatory responses
OT  - lung inflammation
OT  - miR-155
OT  - myeloid cells
OT  - pneumosepsis
COIS- The authors declare no commercial or financial conflict of interest.
EDAT- 2023/10/20 06:42
MHDA- 2023/11/13 06:42
PMCR- 2023/10/19
CRDT- 2023/10/20 00:11
PHST- 2023/05/03 00:00 [received]
PHST- 2023/08/02 00:00 [revised]
PHST- 2023/10/18 00:00 [accepted]
PHST- 2023/11/13 06:42 [medline]
PHST- 2023/10/20 06:42 [pubmed]
PHST- 2023/10/20 00:11 [entrez]
PHST- 2023/10/19 00:00 [pmc-release]
AID - 7324819 [pii]
AID - ftad031 [pii]
AID - 10.1093/femspd/ftad031 [doi]
PST - ppublish
SO  - Pathog Dis. 2023 Jan 17;81:ftad031. doi: 10.1093/femspd/ftad031.