PMID- 37862756
OWN - NLM
STAT- MEDLINE
DCOM- 20231106
LR  - 20231106
IS  - 1873-4235 (Electronic)
IS  - 0956-5663 (Linking)
VI  - 243
DP  - 2024 Jan 1
TI  - An enzymic l-2-hydroxyglutarate biosensor based on l-2-hydroxyglutarate 
      dehydrogenase from Azoarcus olearius.
PG  - 115740
LID - S0956-5663(23)00682-6 [pii]
LID - 10.1016/j.bios.2023.115740 [doi]
AB  - l-2-Hydroxyglutarate (l-2-HG) is a critical signaling and immune metabolite but 
      its excessive accumulation can lead to l-2-hydroxyglutaric aciduria, renal 
      cancer, and other diseases. Development of efficient and high-throughput methods 
      for selective l-2-HG detection is urgently required. In this study, l-2-HG 
      dehydrogenase in Azoarcus olearius BH72 (AoL2HGDH) was screened from ten homologs 
      and identified as an enzyme with high specificity and activity toward l-2-HG 
      dehydrogenation. Then, an enzymatic assay-based l-2-HG-sensing fluorescent 
      reporter, EaLHGFR which consists of AoL2HGDH and resazurin, was developed for the 
      detection of l-2-HG. The response magnitude and limit of detection of EaLHGFR 
      were systematically optimized using a single-factor screening strategy. The 
      optimal biosensor EaLHGFR-2 exhibited a response magnitude of 2189.25 +/- 26.89% 
      and a limit of detection of 0.042 muM. It can accurately detect the concentration 
      of l-2-HG in bacterial and cellular samples as well as human body fluids. 
      Considering its desirable properties, EaLHGFR-2 may be a promising alternative 
      for quantitation of l-2-HG in biological samples.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Hou, Shuang
AU  - Hou S
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China.
FAU - Kang, Zhaoqi
AU  - Kang Z
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China.
FAU - Liu, Yidong
AU  - Liu Y
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China.
FAU - Lu, Chuanjuan
AU  - Lu C
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China.
FAU - Wang, Xia
AU  - Wang X
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China.
FAU - Wang, Qian
AU  - Wang Q
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China.
FAU - Ma, Cuiqing
AU  - Ma C
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China.
FAU - Xu, Ping
AU  - Xu P
AD  - State Key Laboratory of Microbial Metabolism, Shanghai Jiao Tong University, 
      People's Republic of China.
FAU - Gao, Chao
AU  - Gao C
AD  - State Key Laboratory of Microbial Technology, Shandong University, People's 
      Republic of China. Electronic address: jieerbu@sdu.edu.cn.
LA  - eng
PT  - Journal Article
DEP - 20231005
PL  - England
TA  - Biosens Bioelectron
JT  - Biosensors & bioelectronics
JID - 9001289
RN  - 2889-31-8 (alpha-hydroxyglutarate)
RN  - EC 1.1.99.2 (2-hydroxyglutarate dehydrogenase)
RN  - 0 (Glutarates)
RN  - Azoarcus olearius
SB  - IM
MH  - Humans
MH  - *Biosensing Techniques
MH  - Glutarates/metabolism
OTO - NOTNLM
OT  - Biosensor
OT  - Fluorescent assay
OT  - Resazurin
OT  - l-2-Hydroxyglutarate
OT  - l-2-Hydroxyglutarate dehydrogenase
COIS- Declaration of competing interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/10/20 18:43
MHDA- 2023/11/06 06:42
CRDT- 2023/10/20 18:01
PHST- 2023/06/19 00:00 [received]
PHST- 2023/09/21 00:00 [revised]
PHST- 2023/10/03 00:00 [accepted]
PHST- 2023/11/06 06:42 [medline]
PHST- 2023/10/20 18:43 [pubmed]
PHST- 2023/10/20 18:01 [entrez]
AID - S0956-5663(23)00682-6 [pii]
AID - 10.1016/j.bios.2023.115740 [doi]
PST - ppublish
SO  - Biosens Bioelectron. 2024 Jan 1;243:115740. doi: 10.1016/j.bios.2023.115740. Epub 
      2023 Oct 5.