PMID- 37863866
OWN - NLM
STAT- Publisher
LR  - 20231020
IS  - 1573-4927 (Electronic)
IS  - 0006-2928 (Linking)
DP  - 2023 Oct 20
TI  - Exosomal lncRNA LINC02191 Promotes Laryngeal Squamous cell Carcinoma Progression 
      by Targeting miR-204-5p/RAB22A Axis and Regulating PI3K/Akt/mTOR Pathway.
LID - 10.1007/s10528-023-10541-3 [doi]
AB  - Recent research has explored the potential use of serum-derived biomarkers in 
      cancer screening, and mounting evidence has illustrated the pivotal roles of long 
      noncoding RNAs (lncRNAs) in regulating laryngeal squamous cell carcinoma (LSCC) 
      progression. LINC02191 is a newly identified lncRNA and no studies have 
      investigated its role in malignant tumors. This study aims to explore the 
      functions and mechanisms of lncRNA LINC02191 in LSCC. LINC02191 was knocked down 
      in LSCC cells using shRNAs for loss-of-function experiments. RT-qPCR revealed 
      that LINC02191 was upregulated in LSCC patients' serum exosomes, tissues and 
      cells. Western blotting and RT-qPCR were implemented for detecting molecular 
      protein and RNA levels. Colony formation, CCK-8, wound healing and Transwell 
      assays were employed for examining LSCC cell malignant behaviors in vitro. A 
      tumor-bearing mouse model (n = 4/group) was established for examining LINC02191 
      role in vivo. The results showed that LINC02191 silencing hindered LSCC cell 
      proliferation, invasiveness, migration as well as EMT in vitro and impeded 
      tumorigenesis in xenograft mouse model. Luciferase reporter assay was utilized 
      for verifying the interaction between LINC02191, miR-204-5p and RAB22A. Pearson 
      correlation analysis was employed to evaluate their expression correlation in 
      LSCC tissue specimens (N = 30). Mechanistically, LINC02191 upregulated RAB22A by 
      binding to miR-204-5p, and knocking down LINC02191 inhibited PI3K/Akt/mTOR 
      signaling transduction in LSCC cells and tumor-bearing mice. Moreover, RAB22A 
      overexpression reversed LINC02191 depletion-triggered suppression of LSCC cell 
      aggressiveness and inactivation of PI3K/Akt/mTOR signaling. In 
      conclusion, LINC02191 aggravates LSCC by targeting 
      miR-204-5p/RAB22A/PI3K/Akt/mTOR signaling pathway, which indicates that LINC02191 
      may serve as a promising target for LSCC treatment.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer Science+Business 
      Media, LLC, part of Springer Nature.
FAU - Kang, Zhiwei
AU  - Kang Z
AD  - Department of Otolaryngology Head & Neck Surgery, Shanxi Key Laboratory of 
      Otorhinolaryngology Head and Neck Cancer, Shanxi Clinical Medical Research Center 
      for Precision Medicine of Head and Neck Cancer, First Hospital of Shanxi Medical 
      University, Taiyuan, 030001, China.
FAU - Zhang, Chunming
AU  - Zhang C
AD  - Department of Otolaryngology Head & Neck Surgery, Shanxi Key Laboratory of 
      Otorhinolaryngology Head and Neck Cancer, Shanxi Clinical Medical Research Center 
      for Precision Medicine of Head and Neck Cancer, First Hospital of Shanxi Medical 
      University, Taiyuan, 030001, China.
FAU - Huangfu, Hui
AU  - Huangfu H
AD  - Department of Otolaryngology Head & Neck Surgery, Shanxi Key Laboratory of 
      Otorhinolaryngology Head and Neck Cancer, Shanxi Clinical Medical Research Center 
      for Precision Medicine of Head and Neck Cancer, First Hospital of Shanxi Medical 
      University, Taiyuan, 030001, China. hfh1230316@163.com.
LA  - eng
PT  - Journal Article
DEP - 20231020
PL  - United States
TA  - Biochem Genet
JT  - Biochemical genetics
JID - 0126611
SB  - IM
OTO - NOTNLM
OT  - LINC02191
OT  - Laryngeal squamous cell carcinoma
OT  - PI3K/Akt/mTOR pathway
OT  - RAB22A
OT  - miR-204-5p
EDAT- 2023/10/21 05:43
MHDA- 2023/10/21 05:43
CRDT- 2023/10/20 23:04
PHST- 2023/06/26 00:00 [received]
PHST- 2023/10/01 00:00 [accepted]
PHST- 2023/10/21 05:43 [medline]
PHST- 2023/10/21 05:43 [pubmed]
PHST- 2023/10/20 23:04 [entrez]
AID - 10.1007/s10528-023-10541-3 [pii]
AID - 10.1007/s10528-023-10541-3 [doi]
PST - aheadofprint
SO  - Biochem Genet. 2023 Oct 20. doi: 10.1007/s10528-023-10541-3.