PMID- 37866671
OWN - NLM
STAT- MEDLINE
DCOM- 20231216
LR  - 20240306
IS  - 1878-5492 (Electronic)
IS  - 0966-3274 (Linking)
VI  - 81
DP  - 2023 Dec
TI  - Low monocytic HLA-DR expression in critically ill patients of sepsis: An 
      indicator for antimicrobial and/or immunomodulatory intervention.
PG  - 101942
LID - S0966-3274(23)00159-4 [pii]
LID - 10.1016/j.trim.2023.101942 [doi]
AB  - BACKGROUND: Sepsis is a result of suppressed host immune response which leads to 
      fatal multi-organ dysfunctionality. Low frequency of active monocytes or reduced 
      expression of human leukocyte antigen (HLA)-DR on monocytes shows the suppressed 
      immune response in sepsis patients. One of the well-studied markers in patients 
      with sepsis is procalcitonin (PCT). The role of monocytic (m) HLA-DR expression 
      has been monitored in sepsis and is being considered a marker of the severity of 
      interim immuno-depression in these patients. The study describes the impact of 
      HLA-DR expression on monocytes quantitatively using flow cytometry. METHODS: In 
      this prospective study, we quantified monocytes and their HLA-DR expression in 20 
      patients of sepsis admitted to the Intensive Care Unit (ICU). Serum levels of PCT 
      and interleukin (IL)-6 production were also measured in these patients, and the 
      results were compared with those in healthy controls. RESULTS: Monocyte frequency 
      calculated was higher in sepsis patients as compared to healthy controls, 
      however, HLA-DR expressing monocytes were significantly reduced as was the mean 
      fluorescence intensity (MFI) of HLA-DR. Contrastingly, IL-6 and PCT levels were 
      significantly high in sepsis than controls. The results suggest that low HLA-DR 
      expression, combined with PCT, is a better prognostic parameter in the early 
      phase of sepsis. CONCLUSION: Poor recovery of mHLA-DR may serve as an early guide 
      for clinicians to assess the prognosis of sepsis patients and consider 
      immunomodulatory therapy in its management.
CI  - Copyright (c) 2023 Elsevier B.V. All rights reserved.
FAU - Ansari, Mohammad Ahmad
AU  - Ansari MA
AD  - Department of Microbiology, University College of Medical Sciences (University of 
      Delhi) & GTB Hospital, Delhi 110095, India.
FAU - Das, Shukla
AU  - Das S
AD  - Department of Microbiology, University College of Medical Sciences (University of 
      Delhi) & GTB Hospital, Delhi 110095, India. Electronic address: 
      shukladas_123@yahoo.com.
FAU - Rai, Gargi
AU  - Rai G
AD  - Department of Microbiology, University College of Medical Sciences (University of 
      Delhi) & GTB Hospital, Delhi 110095, India.
FAU - Singh, Praveen Kumar
AU  - Singh PK
AD  - Department of Microbiology, University College of Medical Sciences (University of 
      Delhi) & GTB Hospital, Delhi 110095, India.
FAU - Lahan, Shubham
AU  - Lahan S
AD  - University College of Medical Sciences (University of Delhi) & GTB Hospital, 
      Delhi 110095, India.
FAU - Tyagi, Asha
AU  - Tyagi A
AD  - Department of Anesthesiology and Critical Care, University College of Medical 
      Sciences (University of Delhi) & GTB Hospital, Delhi 110095, India.
FAU - Alamer, Edrous
AU  - Alamer E
AD  - Department of Medical Laboratory Technology, College of Applied Medical Sciences, 
      Jazan University, Jazan 45142, Saudi Arabia.
FAU - Dar, Sajad Ahmad
AU  - Dar SA
AD  - Department of Microbiology, University College of Medical Sciences (University of 
      Delhi) & GTB Hospital, Delhi 110095, India; Research and Scientific Studies Unit, 
      College of Nursing, Jazan University, Jazan 45142, Saudi Arabia. Electronic 
      address: darsajad990@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20231020
PL  - Netherlands
TA  - Transpl Immunol
JT  - Transplant immunology
JID - 9309923
RN  - 0 (HLA-DR Antigens)
RN  - 0 (Anti-Infective Agents)
SB  - IM
MH  - Humans
MH  - Monocytes
MH  - Prospective Studies
MH  - Critical Illness
MH  - HLA-DR Antigens/metabolism
MH  - *Sepsis
MH  - *Anti-Infective Agents/metabolism
MH  - Immunomodulation
MH  - Immunity
OTO - NOTNLM
OT  - Human leukocyte antigen
OT  - Interleukin-6
OT  - Monocytes
OT  - Procalcitonin
OT  - Prognosis
COIS- Declaration of Competing Interest The authors declare no conflict of interest.
EDAT- 2023/10/23 00:43
MHDA- 2023/12/17 09:44
CRDT- 2023/10/22 19:32
PHST- 2023/08/26 00:00 [received]
PHST- 2023/10/10 00:00 [revised]
PHST- 2023/10/19 00:00 [accepted]
PHST- 2023/12/17 09:44 [medline]
PHST- 2023/10/23 00:43 [pubmed]
PHST- 2023/10/22 19:32 [entrez]
AID - S0966-3274(23)00159-4 [pii]
AID - 10.1016/j.trim.2023.101942 [doi]
PST - ppublish
SO  - Transpl Immunol. 2023 Dec;81:101942. doi: 10.1016/j.trim.2023.101942. Epub 2023 
      Oct 20.