PMID- 37866940
OWN - NLM
STAT- MEDLINE
DCOM- 20231026
LR  - 20231026
IS  - 1672-173X (Print)
IS  - 1672-173X (Linking)
VI  - 54
IP  - 5
DP  - 2023 Sep
TI  - [Identification of Peripheral Blood GZMK (+) CD8 (+) T Cells As Biomarkers of 
      Alzheimer's Disease Based on Single-Cell Transcriptome].
PG  - 863-873
LID - 10.12182/20230960107 [doi]
AB  - OBJECTIVE: Based on single-cell RNA sequencing (scRNA-seq) to explore immune 
      characteristics in the peripheral blood of patients with Alzheimer's disease (AD) 
      as biomarkers. METHODS: GSE168522, the scRNA-seq dataset of AD peripheral blood 
      immune cells, was downloaded from the Gene Expression Omnibus (GEO) database and 
      was analyzed in the RAD-Blood web server (http://www.bioinform.cn/RAD-Blood/). 
      The changes in blood cell composition in AD patients were analyzed. The abnormal 
      communications between different types of cells in AD patients were investigated 
      by the CellChat R package. RESULTS: There were two kinds of CD8 (+) T cells in 
      the blood of AD patients and healthy individuals, one of which highly expressed 
      granzyme K ( GZMK) (false discovery rate [FDR]<0.05), and the other highly 
      expressed GZMA, GZMB, and GZMH (FDR<0.05). In the blood of AD patients, the 
      content of GZMK (+) CD8 (+) T cells was increased by 32.9% ( P=5.15E-21), their 
      interactions with other cell types were increased, and they might be associated 
      with AD through the abnormal signal transduction of major histocompatibility 
      complex class Ⅰ (MHC-Ⅰ). Erythrocyte provided the main ligands, that are, human 
      leukocyte antigen (HLA) class Ⅰ molecules, including HLA- A, HLA- B, HLA- C, and 
      HLA- E, for the abnormal MHC-Ⅰ signaling pathway of GZMK (+) CD8 (+) T cells. The 
      RESISTIN signaling pathway was specifically enriched in the blood of AD patients. 
      CONCLUSION: The increased content of peripheral blood GZMK (+) CD8 (+) T cells, 
      the increased interaction between GZMK (+) CD8 (+) T cells and erythrocytes, and 
      the enhanced RESISTIN pathway are potential blood biomarkers of AD.
CI  - Copyright(c) by Editorial Board of Journal of Sichuan University (Medical 
      Sciences).
FAU - Duan, Tingting
AU  - Duan T
AD  - Brain Science and Advanced Technology Institute, School of Medicine, Wuhan 
      University of Science and Technology, Wuhan 430081, China.
FAU - Chu, Jinyu
AU  - Chu J
AD  - Brain Science and Advanced Technology Institute, School of Medicine, Wuhan 
      University of Science and Technology, Wuhan 430081, China.
FAU - Hu, Feifei
AU  - Hu F
AD  - Brain Science and Advanced Technology Institute, School of Medicine, Wuhan 
      University of Science and Technology, Wuhan 430081, China.
AD  - Wuhan Asia Heart Hospital, Wuhan University of Science and Technology, Wuhan 
      430022, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Sichuan Da Xue Xue Bao Yi Xue Ban
JT  - Sichuan da xue xue bao. Yi xue ban = Journal of Sichuan University. Medical 
      science edition
JID - 101162609
RN  - EC 3.4.21.- (Granzymes)
RN  - 0 (Resistin)
RN  - 0 (Biomarkers)
SB  - IM
MH  - Humans
MH  - Granzymes
MH  - *Resistin
MH  - Transcriptome
MH  - *Alzheimer Disease/genetics
MH  - CD8-Positive T-Lymphocytes
MH  - Biomarkers
PMC - PMC10579064
OTO - NOTNLM
OT  - Alzheimer's disease
OT  - Blood biomarkers
OT  - CD8+ T cell
OT  - scRNA-seq
COIS- 利益冲突　所有作者均声明不存在利益冲突
EDAT- 2023/10/23 00:43
MHDA- 2023/10/26 06:42
PMCR- 2023/09/20
CRDT- 2023/10/22 21:52
PHST- 2023/10/26 06:42 [medline]
PHST- 2023/10/23 00:43 [pubmed]
PHST- 2023/10/22 21:52 [entrez]
PHST- 2023/09/20 00:00 [pmc-release]
AID - scdxxbyxb-54-5-863 [pii]
AID - 10.12182/20230960107 [doi]
PST - ppublish
SO  - Sichuan Da Xue Xue Bao Yi Xue Ban. 2023 Sep;54(5):863-873. doi: 
      10.12182/20230960107.