PMID- 37867556
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 2296-889X (Print)
IS  - 2296-889X (Electronic)
IS  - 2296-889X (Linking)
VI  - 10
DP  - 2023
TI  - UroVysion() fluorescence in situ hybridization (FISH) possibly has a high 
      positive rate in carcinoma of non-urothelial lineages.
PG  - 1250442
LID - 10.3389/fmolb.2023.1250442 [doi]
LID - 1250442
AB  - Background: Positive UroVysion() fluorescence in situ hybridization (FISH) is 
      generally considered as urothelial carcinoma (UC). We clarify if UroVysion() 
      FISH can be positive in carcinoma of non-urothelial lineages (CNUL), and verify 
      the consistency of urine FISH and histological FISH in CNUL. Methods: All CNUL 
      subjects detected by urine FISH assay due to haematuria from Tongji Hospital were 
      screened. Meanwhile, 2 glandular cystitis and 2 urothelial carcinoma were served 
      as negative or positive control. Paraffin-embedded tissue sections of all 
      subjects were sent to the pathology department for histological FISH detection. 
      Results: A total of 27 patients were included in this study, including 9 with 
      adenocarcinomas, 11 with squamous cell carcinomas, and 7 with other tumour types. 
      The overall positive rate in urine FISH was 64.00% (16/25) in patients with CNUL, 
      77.78% (7/9) in those with adenocarcinoma and 54.55% (6/11) in those with 
      squamous carcinoma. There was a significant difference in the GLP p16 gene 
      deletion rate between UC and CNUL (100% vs. 8.00%, p = 0.017). Histological FISH 
      results showed that the histological results of 19 patients were consistent with 
      their urine FISH results, and only one patient with stage Ⅲa urachal carcinoma 
      had inconsistent histological FISH results (positive) and urine FISH (negative) 
      results. Conclusion: We demonstrated for the first time the application value of 
      FISH in CNUL on urine samples. Positive urine FISH tests indicate not only UC, 
      but also CNUL. UroVysion() FISH possibly has a high positive rate in CNUL. CNUL 
      and UC have different genetic changes shown by FISH.
CI  - Copyright (c) 2023 Ke, Liu, Wan, Hu and Yang.
FAU - Ke, Chunjin
AU  - Ke C
AD  - Department of Urology, Tongji Hospital of Tongji Medical College, Huazhong 
      University of Science and Technology (HUST), Wuhan, China.
FAU - Liu, Xuguang
AU  - Liu X
AD  - Department of Pathology, Tongji Hospital of Tongji Medical College, Huazhong 
      University of Science and Technology (HUST), Wuhan, China.
FAU - Wan, Jie
AU  - Wan J
AD  - Department of Pathology, Tongji Hospital of Tongji Medical College, Huazhong 
      University of Science and Technology (HUST), Wuhan, China.
FAU - Hu, Zhiquan
AU  - Hu Z
AD  - Department of Urology, Tongji Hospital of Tongji Medical College, Huazhong 
      University of Science and Technology (HUST), Wuhan, China.
FAU - Yang, Chunguang
AU  - Yang C
AD  - Department of Urology, Tongji Hospital of Tongji Medical College, Huazhong 
      University of Science and Technology (HUST), Wuhan, China.
LA  - eng
PT  - Journal Article
DEP - 20231005
PL  - Switzerland
TA  - Front Mol Biosci
JT  - Frontiers in molecular biosciences
JID - 101653173
PMC - PMC10586327
OTO - NOTNLM
OT  - CNUL
OT  - adenocarcinoma
OT  - chromosome
OT  - fluorescence in situ hybridization
OT  - squamous cell carcinoma
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/10/23 06:46
MHDA- 2023/10/23 06:47
PMCR- 2023/01/01
CRDT- 2023/10/23 04:25
PHST- 2023/06/30 00:00 [received]
PHST- 2023/09/21 00:00 [accepted]
PHST- 2023/10/23 06:47 [medline]
PHST- 2023/10/23 06:46 [pubmed]
PHST- 2023/10/23 04:25 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 1250442 [pii]
AID - 10.3389/fmolb.2023.1250442 [doi]
PST - epublish
SO  - Front Mol Biosci. 2023 Oct 5;10:1250442. doi: 10.3389/fmolb.2023.1250442. 
      eCollection 2023.