PMID- 37868320
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 1664-462X (Print)
IS  - 1664-462X (Electronic)
IS  - 1664-462X (Linking)
VI  - 14
DP  - 2023
TI  - All nonhomologous chromosomes and rearrangements in Saccharum officinarum x 
      Saccharum spontaneum allopolyploids identified by oligo-based painting.
PG  - 1176914
LID - 10.3389/fpls.2023.1176914 [doi]
LID - 1176914
AB  - Modern sugarcane cultivars (Saccharum spp., 2n = 100~120) are complex polyploids 
      primarily derived from interspecific hybridization between S. officinarum and S. 
      spontaneum. Nobilization is the theory of utilizing wild germplasm in sugarcane 
      breeding, and is the foundation for utilizing S. spontaneum for stress 
      resistance. However, the exact chromosomal transmission remains elusive due to a 
      lack of chromosome-specific markers. Here, we applied chromosome-specific 
      oligonucleotide (oligo)-based probes for identifying chromosomes 1-10 of the F(1) 
      hybrids between S. officinarum and S. spontaneum. Then, S. spontaneum-specific 
      repetitive DNA probes were used to distinguish S. spontaneum in these hybrids. 
      This oligo- fluorescence in situ hybridization (FISH) system proved to be an 
      efficient tool for revealing individual chromosomal inheritance during 
      nobilization. We discovered the complete doubling of S. officinarum-derived 
      chromosomes in most F(1) hybrids. Notably, we also found defective S. 
      officinarum-derived chromosome doubling in the F(1) hybrid Yacheng75-4191, which 
      exhibited 1.5n transmission for all nonhomologous chromosomes. Altogether, these 
      results highlight the presence of variable chromosome transmission in 
      nobilization between S. officinarum and S. spontaneum, including 1.5n + n and 2n 
      + n. These findings provide robust chromosome markers for in-depth studies into 
      the molecular mechanism underlying chromosome doubling during the nobilization, 
      as well as tracing chromosomal inheritance for sugarcane breeding.
CI  - Copyright (c) 2023 Chai, Xue, Lei, Yao, Zhang, Deng and Yu.
FAU - Chai, Jin
AU  - Chai J
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning, China.
AD  - Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning, China.
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou, Fujian, China.
FAU - Xue, Li
AU  - Xue L
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning, China.
AD  - Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning, China.
FAU - Lei, Jiawei
AU  - Lei J
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou, Fujian, China.
FAU - Yao, Wei
AU  - Yao W
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning, China.
AD  - Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning, China.
FAU - Zhang, Muqing
AU  - Zhang M
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning, China.
AD  - Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning, China.
FAU - Deng, Zuhu
AU  - Deng Z
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning, China.
AD  - Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning, China.
AD  - National Engineering Research Center for Sugarcane, Fujian Agriculture and 
      Forestry University, Fuzhou, Fujian, China.
AD  - Key Laboratory of Sugarcane Biology and Genetic Breeding, Ministry of Agriculture 
      and Rural Affairs, Fujian Agriculture and Forestry University, Fuzhou, China.
FAU - Yu, Fan
AU  - Yu F
AD  - State Key Laboratory for Conservation and Utilization of Subtropical 
      Agro-bioresources, Guangxi University, Nanning, China.
AD  - Guangxi Key Laboratory for Sugarcane Biology, Guangxi University, Nanning, China.
LA  - eng
PT  - Journal Article
DEP - 20231006
PL  - Switzerland
TA  - Front Plant Sci
JT  - Frontiers in plant science
JID - 101568200
PMC - PMC10588481
OTO - NOTNLM
OT  - chromosomal inheritance
OT  - interspecific hybridization
OT  - nobilization
OT  - oligo-FISH
OT  - sugarcane
COIS- The authors declare that the research was conducted in the absence of any 
      commercial or financial relationships that could be construed as a potential 
      conflict of interest.
EDAT- 2023/10/23 06:49
MHDA- 2023/10/23 06:50
PMCR- 2023/01/01
CRDT- 2023/10/23 04:36
PHST- 2023/04/06 00:00 [received]
PHST- 2023/09/01 00:00 [accepted]
PHST- 2023/10/23 06:50 [medline]
PHST- 2023/10/23 06:49 [pubmed]
PHST- 2023/10/23 04:36 [entrez]
PHST- 2023/01/01 00:00 [pmc-release]
AID - 10.3389/fpls.2023.1176914 [doi]
PST - epublish
SO  - Front Plant Sci. 2023 Oct 6;14:1176914. doi: 10.3389/fpls.2023.1176914. 
      eCollection 2023.