PMID- 37868950
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231031
IS  - 2590-0064 (Electronic)
IS  - 2590-0064 (Linking)
VI  - 23
DP  - 2023 Dec
TI  - Control of the growth and development of murine preantral follicles in a 
      biomimetic ovary using a decellularized porcine scaffold.
PG  - 100824
LID - 10.1016/j.mtbio.2023.100824 [doi]
LID - 100824
AB  - This study aimed to derive mature oocytes from murine preantral follicles 
      cultured in a biomimetic ovary with a porcine scaffold using decellularization 
      technology. We evaluated the DNA content and the presence of cell and 
      extracellular matrix (ECM) components, including collagen, elastin, and 
      glycosaminoglycans (GAGs), in decellularized (decell) porcine ovaries. The DNA 
      content inthe decell ovarian tissues was approximately 94 % less than that in 
      native tissues (66 +/- 9.8 ng/mg vs. 1139 +/- 269 ng/mg). Furthermore, the ECM 
      component integrity was maintained in the decell ovarian tissue. The soluble 
      collagen concentration of native ovarian tissue (native) was 195.34 +/- 15.13 mug/mg 
      (dry wt.), which was less than 878.6 +/- 8.24 mug/mg for the decell ovarian tissue 
      due to the loss of cellular mass. Hydrogels derived from decell porcine ovaries 
      were prepared to develop an in vitro biomimetic ovary with appropriate ECM 
      concentration (2-6 mg/mL). Scanning electron microscope (SEM) imagining revealed 
      that the complex fiber network and porous structure were maintained in all groups 
      treated with varying ECM concentration (2-6 mg/mL). Furthermore, rheometer 
      analysis indicated that mechanical strength increased with ECM concentration in a 
      dose-dependently. The preantral follicles cultured with 4 mg/mL ECM showed high 
      rates of antral follicle (66 %) and mature oocyte (metaphase II) development 
      (47 %). The preantral follicles cultured in a biomimetic ovary with a decell 
      porcine scaffold showed a higher rate of antral follicle and mature oocytes than 
      those cultured in other biomaterials such as collagen and Matrigel. In mature 
      oocytes derived from antral follicles, meiotic spindles and nuclei were stained 
      using a tubulin antibody and Hoechst, respectively. Two-cell embryos were 
      developed from MII oocytes following parthenogenetic activation. Preantral 
      follicles were cultured in a biomimetic ovary derived from the ECM of a decell 
      porcine ovary, and embryos were generated from MII oocytes. This biomimetic ovary 
      could contribute to restoring fertility in infertile women with reduced ovarian 
      function, benefit mating efforts for endangered species, and maintain animals 
      with valuable genetic traits.
CI  - (c) 2023 Published by Elsevier Ltd.
FAU - Park, Eun Young
AU  - Park EY
AD  - Department of Biotechnology, College of Life and Applied Sciences, Yeungnam 
      University, Gyeongsan, 38541, South Korea.
FAU - Park, Jin Hee
AU  - Park JH
AD  - Department of Biotechnology, College of Life and Applied Sciences, Yeungnam 
      University, Gyeongsan, 38541, South Korea.
FAU - Mai, Nhu Thi Quynh
AU  - Mai NTQ
AD  - Department of Biotechnology, Chonnam National University, Yeosu, 59626, Republic 
      of Korea.
FAU - Moon, Byoung-San
AU  - Moon BS
AD  - Department of Biotechnology, Chonnam National University, Yeosu, 59626, Republic 
      of Korea.
FAU - Choi, Jung Kyu
AU  - Choi JK
AD  - Department of Biotechnology, College of Life and Applied Sciences, Yeungnam 
      University, Gyeongsan, 38541, South Korea.
LA  - eng
PT  - Journal Article
DEP - 20231007
PL  - England
TA  - Mater Today Bio
JT  - Materials today. Bio
JID - 101757228
PMC - PMC10587716
OTO - NOTNLM
OT  - Biomimetic ovary
OT  - Decellularization technology
OT  - Extracellular matrix
OT  - Ovarian follicle
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/10/23 06:49
MHDA- 2023/10/23 06:50
PMCR- 2023/10/07
CRDT- 2023/10/23 04:45
PHST- 2023/07/14 00:00 [received]
PHST- 2023/09/22 00:00 [revised]
PHST- 2023/09/28 00:00 [accepted]
PHST- 2023/10/23 06:50 [medline]
PHST- 2023/10/23 06:49 [pubmed]
PHST- 2023/10/23 04:45 [entrez]
PHST- 2023/10/07 00:00 [pmc-release]
AID - S2590-0064(23)00284-3 [pii]
AID - 100824 [pii]
AID - 10.1016/j.mtbio.2023.100824 [doi]
PST - epublish
SO  - Mater Today Bio. 2023 Oct 7;23:100824. doi: 10.1016/j.mtbio.2023.100824. 
      eCollection 2023 Dec.