PMID- 37870167 OWN - NLM STAT- MEDLINE DCOM- 20231229 LR - 20240106 IS - 2198-3844 (Electronic) IS - 2198-3844 (Linking) VI - 10 IP - 36 DP - 2023 Dec TI - Highly-Specific Single-Stranded Oligonucleotides and Functional Nanoprobes for Clinical Determination of Chlamydia Trachomatis and Neisseria Gonorrhoeae Infections. PG - e2304009 LID - 10.1002/advs.202304009 [doi] LID - 2304009 AB - Early detection of Chlamydia trachomatis (CT) and Neisseria gonorrhoeae (NG) is the key to controlling the spread of these bacterial infections. An important step in developing biosensors involves identifying reliable sensing probes against specific genetic targets for CT and NG. Here, the authors have designed single-stranded oligonucleotides (ssDNAs) targeting mutually conserved genetic regions of cryptic plasmid and chromosomal DNA of both CT and NG. The 5'- and 3'- ends of these ssDNAs are differentially functionalized with thiol groups and coupled with gold nanoparticles (AuNP) to develop absorbance-based assay. The AuNPs agglomerate selectively in the presence of its target DNA sequence and demonstrate a change in their surface plasmon resonance. The optimized assay is then used to detect both CT and NG DNA extracted from 60 anonymized clinical samples with a clinical sensitivity of approximately 100%. The limit of detection of the assays are found to be 7 and 5 copies/microL for CT and NG respectively. Furthermore, it can successfully detect the DNA levels of these two bacteria without the need for DNA extraction and via a lateral flow-based platform. These assays thus hold the potential to be employed in clinics for rapid and efficient monitoring of sexually transmitted infections. CI - (c) 2023 The Authors. Advanced Science published by Wiley-VCH GmbH. FAU - Dighe, Ketan AU - Dighe K AD - Department of Pediatrics, Centre of Blood Oxygen Transport & Hemostasis, University of Maryland Baltimore School of Medicine, Baltimore, Maryland, 21201, USA. AD - Department of Chemical & Biochemical Engineering, University of Maryland Baltimore County, Baltimore County, Maryland, 21250, USA. AD - Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. FAU - Moitra, Parikshit AU - Moitra P AD - Department of Pediatrics, Centre of Blood Oxygen Transport & Hemostasis, University of Maryland Baltimore School of Medicine, Baltimore, Maryland, 21201, USA. AD - Department of Nuclear Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. FAU - Gunaseelan, Nivetha AU - Gunaseelan N AD - Department of Pediatrics, Centre of Blood Oxygen Transport & Hemostasis, University of Maryland Baltimore School of Medicine, Baltimore, Maryland, 21201, USA. AD - Department of Chemical & Biochemical Engineering, University of Maryland Baltimore County, Baltimore County, Maryland, 21250, USA. AD - Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. FAU - Alafeef, Maha AU - Alafeef M AD - Department of Pediatrics, Centre of Blood Oxygen Transport & Hemostasis, University of Maryland Baltimore School of Medicine, Baltimore, Maryland, 21201, USA. AD - Department of Chemical & Biochemical Engineering, University of Maryland Baltimore County, Baltimore County, Maryland, 21250, USA. AD - Department of Nuclear Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. FAU - Jensen, Tor AU - Jensen T AD - Cancer Center at Illinois, University of Illinois Urbana-Champaign, 405 N. Mathews Ave., Urbana, IL, 61801-2325, USA. FAU - Rafferty, Carla AU - Rafferty C AD - Department of Family Medicine, Carle Health, 1818 E Windsor Rd., Urbana, IL, 61802, USA. FAU - Pan, Dipanjan AU - Pan D AUID- ORCID: 0000-0003-0175-4704 AD - Department of Pediatrics, Centre of Blood Oxygen Transport & Hemostasis, University of Maryland Baltimore School of Medicine, Baltimore, Maryland, 21201, USA. AD - Department of Chemical & Biochemical Engineering, University of Maryland Baltimore County, Baltimore County, Maryland, 21250, USA. AD - Department of Biomedical Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. AD - Department of Nuclear Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. AD - Department of Materials Science and Engineering, The Pennsylvania State University, University Park, PA, 16802, USA. AD - Huck Institutes of the Life Sciences, 101 Huck Life Sciences Building, University Park, PA, 16802, USA. LA - eng GR - TP210376/Department of Defense/ GR - 75D30122C15492/Centers for Disease Control and Prevention Foundation/ GR - 2153091/National Science Foundation/ GR - 2229986/National Science Foundation/ PT - Journal Article DEP - 20231023 PL - Germany TA - Adv Sci (Weinh) JT - Advanced science (Weinheim, Baden-Wurttemberg, Germany) JID - 101664569 RN - 7440-57-5 (Gold) RN - 0 (Oligonucleotides) RN - 9007-49-2 (DNA) SB - IM MH - Humans MH - Neisseria gonorrhoeae/genetics MH - Chlamydia trachomatis/genetics MH - Gold MH - Oligonucleotides MH - *Chlamydia Infections/diagnosis/microbiology MH - Sensitivity and Specificity MH - *Metal Nanoparticles MH - *Gonorrhea/diagnosis/microbiology MH - DNA PMC - PMC10754082 OTO - NOTNLM OT - Chlamydia trachomatis OT - Neisseria gonorrhoeae OT - gold nanoparticles OT - lateral flow assay OT - point-of-care OT - single-stranded oligonucleotides COIS- Prof. Dipanjan Pan is the founder/cofounder of three University based start-ups. None of these entities, however, supported this work. EDAT- 2023/10/23 12:41 MHDA- 2023/12/29 06:43 PMCR- 2023/10/23 CRDT- 2023/10/23 07:32 PHST- 2023/09/18 00:00 [revised] PHST- 2023/06/18 00:00 [received] PHST- 2023/12/29 06:43 [medline] PHST- 2023/10/23 12:41 [pubmed] PHST- 2023/10/23 07:32 [entrez] PHST- 2023/10/23 00:00 [pmc-release] AID - ADVS6623 [pii] AID - 10.1002/advs.202304009 [doi] PST - ppublish SO - Adv Sci (Weinh). 2023 Dec;10(36):e2304009. doi: 10.1002/advs.202304009. Epub 2023 Oct 23.