PMID- 37871386
OWN - NLM
STAT- MEDLINE
DCOM- 20231121
LR  - 20231121
IS  - 1090-2414 (Electronic)
IS  - 0147-6513 (Linking)
VI  - 267
DP  - 2023 Nov 15
TI  - Associations between phthalate metabolites and cytokines in the follicular fluid 
      of women undergoing in vitro fertilization.
PG  - 115616
LID - S0147-6513(23)01120-X [pii]
LID - 10.1016/j.ecoenv.2023.115616 [doi]
AB  - Many studies have showed that phthalates have reproductive and embryonic 
      toxicity, while the potential mechanisms are mostly unknown. Inflammation may 
      play a mediating part in phthalate exposure and adverse reproductive endpoints. A 
      cross-sectional survey was conducted to investigate the associations of phthalate 
      metabolites with inflammatory cytokines in the follicular fluid (FF) of women 
      undergoing in vitro fertilization (IVF). We determined the levels of eight 
      phthalate metabolites and five cytokines in the FF of 76 women, including 
      interleukin (IL)- 6, IL-8, IL-10, monocyte chemoattractant protein-1 (MCP-1) and 
      tumor necrosis factor-alpha (TNF-alpha). The associations of individual phthalate 
      exposure with cytokines in FF samples were explored by multiple linear 
      regression. We further evaluated the combined effects of multiple phthalate 
      exposures on FF levels of cytokines by using Bayesian kernel machine regression 
      (BKMR) models. We found that there was a positive relationship between mono-ethyl 
      phthalate (MEP) and IL-6 in the FF (percent change:12.4%; 95% CI: 1.3%, 24.9%). 
      In contrast, elevated mono-benzyl phthalate (MBzP), mono(2-ethylhexyl) phthalate 
      (MEHP) and %MEHP levels were associated with decreased MCP-1. In the BKMR models, 
      phthalate metabolite mixtures were positively associated with TNF-alpha when the 
      mixtures were lower than 65th percentile compared with their medians. In the 
      stratified analyses, MEHP was inversely associated with MCP-1 among women with 
      BMI >/= 23 kg/m(2) (test for interaction <0.05). Our results suggest that certain 
      phthalate metabolites or their mixtures may alter levels of inflammatory 
      cytokines in the FF, and further research is necessary to elucidate the 
      mechanisms underlying the relationship between phthalates exposure, ovarian 
      dysfunction and adverse pregnancy outcomes.
CI  - Copyright (c) 2023 The Authors. Published by Elsevier Inc. All rights reserved.
FAU - Wang, Yi
AU  - Wang Y
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Du, Yao-Yao
AU  - Du YY
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Yao, Wen
AU  - Yao W
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Deng, Tao-Ran
AU  - Deng TR
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Guo, Na
AU  - Guo N
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Yin, Li
AU  - Yin L
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Yuan, Xiao-Qiong
AU  - Yuan XQ
AD  - Center for Reproductive Medicine, The Third Affiliated Hospital of Zhengzhou 
      University, Zhengzhou, Henan, China.
FAU - Guo, Qing-Chun
AU  - Guo QC
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Li, Juan
AU  - Li J
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Liao, Hong-Mei
AU  - Liao HM
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Qin, Dan-Yu
AU  - Qin DY
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China.
FAU - Li, Yu-Feng
AU  - Li YF
AD  - Reproductive Medicine Center, Tongji Hospital, Tongji Medical College, Huazhong 
      University of Science and Technology, Wuhan, Hubei, China. Electronic address: 
      tjlyf66@126.com.
LA  - eng
PT  - Journal Article
DEP - 20231021
PL  - Netherlands
TA  - Ecotoxicol Environ Saf
JT  - Ecotoxicology and environmental safety
JID - 7805381
RN  - 0 (Cytokines)
RN  - 6O7F7IX66E (phthalic acid)
RN  - 0 (Tumor Necrosis Factor-alpha)
RN  - FU2EWB60RT (mono-(2-ethylhexyl)phthalate)
RN  - 0 (Interleukin-6)
SB  - IM
MH  - Pregnancy
MH  - Female
MH  - Humans
MH  - *Cytokines
MH  - *Tumor Necrosis Factor-alpha
MH  - Bayes Theorem
MH  - Cross-Sectional Studies
MH  - Follicular Fluid
MH  - Interleukin-6
MH  - Fertilization in Vitro
OTO - NOTNLM
OT  - Bayesian kernel machine regression
OT  - Cytokines
OT  - Follicular fluid
OT  - IVF women
OT  - Inflammation
OT  - Phthalates
COIS- Declaration of Competing Interest The authors declare that they have no known 
      competing financial interests or personal relationships that could have appeared 
      to influence the work reported in this paper.
EDAT- 2023/10/24 00:41
MHDA- 2023/11/21 06:42
CRDT- 2023/10/23 18:03
PHST- 2023/05/08 00:00 [received]
PHST- 2023/10/15 00:00 [revised]
PHST- 2023/10/17 00:00 [accepted]
PHST- 2023/11/21 06:42 [medline]
PHST- 2023/10/24 00:41 [pubmed]
PHST- 2023/10/23 18:03 [entrez]
AID - S0147-6513(23)01120-X [pii]
AID - 10.1016/j.ecoenv.2023.115616 [doi]
PST - ppublish
SO  - Ecotoxicol Environ Saf. 2023 Nov 15;267:115616. doi: 
      10.1016/j.ecoenv.2023.115616. Epub 2023 Oct 21.