PMID- 3787170
OWN - NLM
STAT- MEDLINE
DCOM- 19861229
LR  - 20190908
IS  - 0036-553X (Print)
IS  - 0036-553X (Linking)
VI  - 37
IP  - 3
DP  - 1986 Sep
TI  - Glycolytic activity in human red cell populations separated by a combination of 
      density and counterflow centrifugation. Evidence for an improved separation of 
      red cells according to age.
PG  - 189-95
AB  - Human red blood cells were separated by a discontinuous Percoll density gradient 
      into fractions of increasing density. Red cells comprising the lowest and highest 
      density fractions, respectively, were subsequently separated according to mean 
      cell volume (MCV) by means of counterflow centrifugation. The activities of 4 red 
      cell age-dependent enzymes (hexokinase (HK), pyruvate kinase (PK), 
      glucose-6-phosphate dehydrogenase (G6PD) and aspartate aminotransferase (ASAT) 
      were highest in the red cell fraction with low density/large MCV, although the 
      difference from red cell enzyme activities in the total low density fraction was 
      not significant. These 4 enzyme activities were lowest in the fraction of red 
      cells with high density/small MCV. The relative activities of the enzymes in the 
      high density/small MCV fraction, as compared to the unseparated cell population, 
      were: HK (58%), PK (49%), G6PD (53%) and ASAT (28%). These activities were all 
      significantly lower than those measured in the total high density red cell 
      fraction. The rates of lactate production in the low density/large MCV cells 
      (0.89 +/- 0.15 mumol X min-1 X 10(-11) cells) is approximately 3-fold higher than 
      in high density/small MCV cells (0.33 +/- 0.03 mumol X min-1 X 10(-11) cells). 
      This latter value is 1.8-fold lower than the rate of lactate production in the 
      total high density red cell fraction (0.59 +/- 0.14 mumol X min-1 X 10(-11) 
      cells) and is, in contrast to lactate production in other density/size fractions, 
      insensitive to phosphate as a metabolic stimulus. It is argued that the 
      combination of density gradient and counter-flow centrifugation offers a greater 
      potential for obtaining an old red cell population than classical red cell 
      density centrifugation alone.
FAU - Jansen, G
AU  - Jansen G
FAU - Hepkema, B G
AU  - Hepkema BG
FAU - van der Vegt, S G
AU  - van der Vegt SG
FAU - Staal, G E
AU  - Staal GE
LA  - eng
PT  - Journal Article
PL  - Denmark
TA  - Scand J Haematol
JT  - Scandinavian journal of haematology
JID - 0404507
RN  - 0 (Lactates)
RN  - 33X04XA5AT (Lactic Acid)
SB  - IM
MH  - Cell Separation/*methods
MH  - Centrifugation, Density Gradient
MH  - *Erythrocyte Aging
MH  - Erythrocyte Indices
MH  - Erythrocyte Volume
MH  - Erythrocytes/enzymology/*metabolism
MH  - *Glycolysis
MH  - Humans
MH  - Lactates/biosynthesis
MH  - Lactic Acid
EDAT- 1986/09/01 00:00
MHDA- 1986/09/01 00:01
CRDT- 1986/09/01 00:00
PHST- 1986/09/01 00:00 [pubmed]
PHST- 1986/09/01 00:01 [medline]
PHST- 1986/09/01 00:00 [entrez]
AID - 10.1111/j.1600-0609.1986.tb02296.x [doi]
PST - ppublish
SO  - Scand J Haematol. 1986 Sep;37(3):189-95. doi: 10.1111/j.1600-0609.1986.tb02296.x.