PMID- 37874495
OWN - NLM
STAT- MEDLINE
DCOM- 20231109
LR  - 20231109
IS  - 1573-8221 (Electronic)
IS  - 0007-4888 (Linking)
VI  - 175
IP  - 5
DP  - 2023 Sep
TI  - Knockdown of Long Noncoding RNA CCAT2 Suppresses Malignant Phenotype in Human 
      Laryngeal Squamous Cell Carcinoma.
PG  - 673-680
LID - 10.1007/s10517-023-05924-w [doi]
AB  - This study aimed to explore the biological role and mechanism underlying the 
      effects of colon cancer-associated transcript 2 (CCAT2), a long noncoding RNA 
      (lncRNA) in human laryngeal squamous cell carcinoma (LSCC). CCAT2 expression 
      levels in clinical LSCC samples and TU-212 cell line were evaluated by 
      quantitative real-time PCR. The correlation of CCAT2 expression level with 
      clinical-pathological characteristics of patients and their prognosis was 
      analyzed. The functional role of CCAT2 in human LSCC was assessed by Cell 
      Counting Kit-8, Transwell assay, flow cytometric analysis, and LSCC xenograft 
      experiment in vivo. The expression of potential targeted proteins was detected by 
      Western blotting and immunohistochemistry. We found that expression of CCAT2 was 
      significantly elevated in LSCC tissues and TU-212 cells (p<0.05). Survival 
      analysis showed that LSCC patients with high expression of CCAT2 had a shorter 
      5-year overall survival rate than those with low expression (p<0.05). In 
      addition, CCAT2 silencing with short hairpin RNA significantly decreased the 
      proliferative and invasive potential of TU-212 cells (p<0.05) and promoted their 
      apoptosis. In Nude mice, CCAT2 knockdown suppressed the growth of tumor and 
      decreased its volume and weight in comparison with the controls (p<0.05). In 
      TU-212 cells, CCAT2 silencing with short hairpin RNA significantly down-regulated 
      the expression of beta-catenin and CDK8 (p<0.05). Thus, knockdown of CCAT2 
      suppresses proliferation and invasion of the cells and inhibits Wnt/beta-catenin 
      signaling pathway in LSCC, which indicates novel therapeutic targets and 
      prognostic indicators in patients with LSCC.
CI  - (c) 2023. Springer Science+Business Media, LLC, part of Springer Nature.
FAU - Li, M
AU  - Li M
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - An, J
AU  - An J
AD  - Department of Otolaryngology, XuZhou Central Hospital, XuZhou Clinical School of 
      Xuzhou Medical University, Xuzhou, Jiangsu, China.
FAU - Ren, H
AU  - Ren H
AD  - Department of Infection Control, The First Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
FAU - Gui, J
AU  - Gui J
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Wang, H
AU  - Wang H
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Wu, S
AU  - Wu S
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Wu, R
AU  - Wu R
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China.
FAU - Xiao, H
AU  - Xiao H
AD  - Department of Otolaryngology-Head and Neck Surgery, The Second Affiliated 
      Hospital of Harbin Medical University, Harbin, China. xiaohuiyw2@163.com.
FAU - Wang, L
AU  - Wang L
AD  - Department of Orthopedics, The Second Affiliated Hospital of Harbin Medical 
      University, Harbin, China.
LA  - eng
PT  - Journal Article
DEP - 20231024
PL  - United States
TA  - Bull Exp Biol Med
JT  - Bulletin of experimental biology and medicine
JID - 0372557
RN  - 0 (MicroRNAs)
RN  - 0 (RNA, Long Noncoding)
RN  - 0 (RNA, Small Interfering)
RN  - 0 (long non-coding RNA CCAT2, human)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Mice
MH  - Cell Line, Tumor
MH  - Cell Proliferation/genetics
MH  - *Colonic Neoplasms/genetics
MH  - Gene Expression Regulation, Neoplastic
MH  - *Head and Neck Neoplasms/genetics
MH  - Mice, Nude
MH  - *MicroRNAs/genetics
MH  - Phenotype
MH  - *RNA, Long Noncoding/genetics
MH  - RNA, Small Interfering
MH  - Squamous Cell Carcinoma of Head and Neck/genetics
OTO - NOTNLM
OT  - apoptosis
OT  - cyclin-dependent kinase 8
OT  - laryngeal squamous cell carcinoma
OT  - long noncoding RNA colon cancer-associated transcript 2 (CCAT2)
OT  - proliferation
EDAT- 2023/10/24 12:43
MHDA- 2023/11/02 12:44
CRDT- 2023/10/24 11:14
PHST- 2022/06/27 00:00 [received]
PHST- 2023/11/02 12:44 [medline]
PHST- 2023/10/24 12:43 [pubmed]
PHST- 2023/10/24 11:14 [entrez]
AID - 10.1007/s10517-023-05924-w [pii]
AID - 10.1007/s10517-023-05924-w [doi]
PST - ppublish
SO  - Bull Exp Biol Med. 2023 Sep;175(5):673-680. doi: 10.1007/s10517-023-05924-w. Epub 
      2023 Oct 24.