PMID- 37875416
OWN - NLM
STAT- MEDLINE
DCOM- 20231027
LR  - 20231027
IS  - 1872-2059 (Electronic)
IS  - 1000-8713 (Print)
IS  - 1000-8713 (Linking)
VI  - 41
IP  - 10
DP  - 2023 Oct
TI  - [Surface-modified microchip electrophoretic separation and analysis of functional 
      components in health care products].
PG  - 937-948
LID - 10.3724/SP.J.1123.2023.08019 [doi]
AB  - Microchip electrophoresis (MCE) is widely applied in food, environment, medicine, 
      and other fields, owing to its high separation efficiency, low consumption of 
      reagents and samples, and ease of integrating multiple operating units. Polymer 
      microchip materials like cycloolefin copolymer (COC) are low-cost and easy to 
      fabricate. However, their practical applications are limited by the non-specific 
      adsorption on channel surface during electrophoresis and the instability of 
      electroosmotic flow. These shortcomings can be solved by COC surface 
      modification. In this study, a static coating and dynamic/static coating combined 
      strategy was used to develop a channel-surface-modified COC microchip. Combined 
      with laser-induced fluorescence (LIF) detection, a MCE-LIF separation and 
      analysis method was developed for detecting functional components in health care 
      products. The separation performance of MCE was improved by the static coating 
      microchannel surface modification method. The static coating was constructed by 
      hydrophobic amino acid adsorption, glutaraldehyde immobilization, and hydrophilic 
      amino acid functionalization on the COC microchannel surface. The separation 
      performance of MCE was improved by microchannel surface modification combined 
      with dynamic/static coating. The static coating was constructed by valine 
      adsorption, carboxyl activation, and ethylenediamine functionalization on the COC 
      microchannel surface. The dynamic coating is automatically formed by introducing 
      a buffer solution containing hydroxypropyl methylcellulose and sodium dodecyl 
      sulfate into the microchannel. The physical and chemical properties of 
      surface-modified microchannels and the factors governing electrophoretic 
      separation were studied. Combined with LIF detection, the MCE-LIF separation and 
      analysis of lysine and gamma-aminobutyric acid present in children's health care 
      products, as well as aspartic acid and taurine in sport drinks, were developed. 
      The recoveries of lysine and gamma-aminobutyric acid in children's health care 
      products were 84.8%-118%, and the relative standard deviations (RSDs) were less 
      than 7.2% (n=3). The recoveries of aspartic acid and taurine in sport drinks were 
      97.5%-118%, and the RSDs were less than 6.4% (n=3). The analysis results are 
      consistent with the HPLC results, and the method has potential for application in 
      the separation and analysis of anionic amino acids in health care products.
FAU - Lau, Wai-Chun
AU  - Lau WC
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.
FAU - Chen, Ya-Li
AU  - Chen YL
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.
FAU - Xia, Ling
AU  - Xia L
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.
FAU - Xiao, Xiao-Hua
AU  - Xiao XH
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.
FAU - Li, Gong-Ke
AU  - Li GK
AD  - School of Chemistry, Sun Yat-sen University, Guangzhou 510006, China.
LA  - chi
PT  - English Abstract
PT  - Journal Article
PL  - China
TA  - Se Pu
JT  - Se pu = Chinese journal of chromatography
JID - 9424804
RN  - 30KYC7MIAI (Aspartic Acid)
RN  - K3Z4F929H6 (Lysine)
RN  - 0 (Polymers)
RN  - 0 (Amino Acids)
RN  - 1EQV5MLY3D (Taurine)
RN  - 56-12-2 (gamma-Aminobutyric Acid)
SB  - IM
MH  - Child
MH  - Humans
MH  - *Electrophoresis, Microchip/methods
MH  - Aspartic Acid
MH  - Lysine
MH  - Polymers
MH  - Amino Acids
MH  - Taurine
MH  - gamma-Aminobutyric Acid
PMC - PMC10598572
OTO - NOTNLM
OT  - cycloolefin copolymer (COC)
OT  - electrophoresis
OT  - health care products
OT  - microchips
OT  - surface modification
EDAT- 2023/10/25 00:42
MHDA- 2023/10/27 06:42
PMCR- 2023/10/08
CRDT- 2023/10/24 22:23
PHST- 2023/10/27 06:42 [medline]
PHST- 2023/10/25 00:42 [pubmed]
PHST- 2023/10/24 22:23 [entrez]
PHST- 2023/10/08 00:00 [pmc-release]
AID - 10.3724/SP.J.1123.2023.08019 [doi]
PST - ppublish
SO  - Se Pu. 2023 Oct;41(10):937-948. doi: 10.3724/SP.J.1123.2023.08019.