PMID- 37876415
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231026
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 10
DP  - 2023 Oct
TI  - To reduce cytotoxicity when testing the virucidal activity of chemical 
      disinfectants and biocides: The "T-25 method" as an alternative to 
      "large-volume-plating".
PG  - e20728
LID - 10.1016/j.heliyon.2023.e20728 [doi]
LID - e20728
AB  - When testing the virucidal activity of biocides, the non-inactivated residual 
      virus is titrated on cell cultures by the end point dilution method on 96-well 
      tissue culture plates. However, residues of the biocide to be tested also come 
      into contact with the cell cultures in varying concentrations and thus can lead 
      to cytotoxic effects even at high levels of dilution. In the European standards 
      for testing biocides, in particular disinfectants, methods such as 
      Large-Volume-Plating (LVP) method and, in some guidelines, gel filtration 
      procedures are described for reducing cytotoxic effects in the case of highly 
      cytotoxic products, if the classical dilution method proves to be impractical. In 
      order to enable the testing of highly cytotoxic biocides for their activity 
      against viruses, an alternative method for reducing cytotoxicity is introduced, 
      which is based on a procedure of isolating infectious viruses from cytotoxic 
      patients' materials such as stool and can be applied when the other methods fail.
CI  - (c) 2023 HygCen Germany GmbH.
FAU - Rheinbaben, F V
AU  - Rheinbaben FV
AD  - HygCen Germany GmbH, Schwerin, Germany.
FAU - Kohnlein, J
AU  - Kohnlein J
AD  - HygCen Germany GmbH, Schwerin, Germany.
FAU - Schmidt, N
AU  - Schmidt N
AD  - HygCen Germany GmbH, Schwerin, Germany.
FAU - Hildebrandt, C
AU  - Hildebrandt C
AD  - HygCen Germany GmbH, Schwerin, Germany.
FAU - Werner, S
AU  - Werner S
AD  - HygCen Germany GmbH, Schwerin, Germany.
LA  - eng
PT  - Journal Article
DEP - 20231009
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10590779
OTO - NOTNLM
OT  - Biocide
OT  - Cytotoxicity
OT  - Neutralization
OT  - T-25 method
COIS- I wish to submit an revised original research article entitled "To reduce 
      cytotoxicity when testing the virucidal activity of chemical disinfectants and 
      biocides: The "T-25 method" as an alternative to "Large-Volume Plating"" for 
      consideration by the Journal of Virological Methods. I confirm that this work is 
      original and has not been published elsewhere, nor is it currently under 
      consideration for publication elsewhere. All comments of the first review have 
      been revised in this version. We have no conflicts of interest to disclose.
EDAT- 2023/10/25 06:42
MHDA- 2023/10/25 06:43
PMCR- 2023/10/09
CRDT- 2023/10/25 03:49
PHST- 2023/03/01 00:00 [received]
PHST- 2023/09/28 00:00 [revised]
PHST- 2023/10/04 00:00 [accepted]
PHST- 2023/10/25 06:43 [medline]
PHST- 2023/10/25 06:42 [pubmed]
PHST- 2023/10/25 03:49 [entrez]
PHST- 2023/10/09 00:00 [pmc-release]
AID - S2405-8440(23)07936-7 [pii]
AID - e20728 [pii]
AID - 10.1016/j.heliyon.2023.e20728 [doi]
PST - epublish
SO  - Heliyon. 2023 Oct 9;9(10):e20728. doi: 10.1016/j.heliyon.2023.e20728. eCollection 
      2023 Oct.