PMID- 37876448
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231026
IS  - 2405-8440 (Print)
IS  - 2405-8440 (Electronic)
IS  - 2405-8440 (Linking)
VI  - 9
IP  - 10
DP  - 2023 Oct
TI  - Anti-inflammatory of disenecionyl cis-khellactone in LPS-stimulated 
      RAW264.7 cells and the its inhibitory activity on soluble epoxide hydrolase.
PG  - e21032
LID - 10.1016/j.heliyon.2023.e21032 [doi]
LID - e21032
AB  - The objective of the present study was to investigate anti-inflammatory effects 
      of disenecionyl cis-khellactone (DK) isolated from Peucedanum japonicum Thunberg, 
      a traditional edible plant, in RAW264.7 cells stimulated with lipopolysaccharide 
      (LPS). Anti-inflammatory effects of DK were analyzed using various techniques, 
      including NO assay, Western blot analysis, enzyme-linked immunosorbent assay 
      (ELISA), real-time PCR, and immunofluorescence staining. It was revealed that DK 
      reduced the production of pro-inflammatory cytokines including Monocyte 
      chemoattractant protein-1 (MCP-1), Tumor necrosis factor-alpha (TNF-alpha), Interleukin 
      1beta (IL-1beta), and Interleukin 6 (IL-6) in RAW264.7 cells stimulated with LPS. It 
      was revealed that DK effectively downregulated expression levels of iNOS and 
      COX-2 due to inhibition of NF-kappaB activation and suppressing the phosphorylation 
      of p38 and jun N-terminal kinase (JNK) mitogen-activated protein kinase (MAPK) 
      phosphorylation. Also, soluble epoxide hydrolase activity and expression were 
      decreased by the proinflammatory inhibitor, DK. Finally, findings of this study 
      suggest that DK isolated from P. japonicum might have potential as a therapeutic 
      candidate for inflammatory diseases.
CI  - (c) 2023 The Authors.
FAU - Park, Ji Hyeon
AU  - Park JH
AD  - Institute of Health Science, Jeonju University, 303 Cheonjam-ro, Wansan-gu, 
      Jeonju-si, Jeollabuk-do, 55069, Republic of Korea.
FAU - Kim, Jang Hoon
AU  - Kim JH
AD  - Department of Herbal Crop Research, National Institute of Horticultural & Herbal 
      Science, RDA, Eumsung, 27709, Republic of Korea.
FAU - Jang, Seon Il
AU  - Jang SI
AD  - Institute of Health Science, Jeonju University, 303 Cheonjam-ro, Wansan-gu, 
      Jeonju-si, Jeollabuk-do, 55069, Republic of Korea.
AD  - Department of Health Management, Jeonju University, 303 Cheonjam-ro, Wansan-gu, 
      Jeonju-si, Jeollabuk-do, 55069, Republic of Korea.
FAU - Cho, Byoung Ok
AU  - Cho BO
AD  - Institute of Health Science, Jeonju University, 303 Cheonjam-ro, Wansan-gu, 
      Jeonju-si, Jeollabuk-do, 55069, Republic of Korea.
LA  - eng
PT  - Journal Article
DEP - 20231014
PL  - England
TA  - Heliyon
JT  - Heliyon
JID - 101672560
PMC - PMC10590947
OTO - NOTNLM
OT  - Anti-inflammation
OT  - Disenecionyl cis-khellactone
OT  - MAPK
OT  - NF-kappaB
OT  - Peucedanum japonicum thunberg
OT  - Soluble epoxide hydrolase
COIS- The authors declare that they have no known competing financial interests or 
      personal relationships that could have appeared to influence the work reported in 
      this paper.
EDAT- 2023/10/25 06:42
MHDA- 2023/10/25 06:43
PMCR- 2023/10/14
CRDT- 2023/10/25 03:49
PHST- 2023/07/25 00:00 [received]
PHST- 2023/10/12 00:00 [revised]
PHST- 2023/10/13 00:00 [accepted]
PHST- 2023/10/25 06:43 [medline]
PHST- 2023/10/25 06:42 [pubmed]
PHST- 2023/10/25 03:49 [entrez]
PHST- 2023/10/14 00:00 [pmc-release]
AID - S2405-8440(23)08240-3 [pii]
AID - e21032 [pii]
AID - 10.1016/j.heliyon.2023.e21032 [doi]
PST - epublish
SO  - Heliyon. 2023 Oct 14;9(10):e21032. doi: 10.1016/j.heliyon.2023.e21032. 
      eCollection 2023 Oct.