PMID- 37879852
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20240301
IS  - 2005-3606 (Print)
IS  - 2005-5447 (Electronic)
IS  - 2005-3606 (Linking)
VI  - 17
IP  - 1
DP  - 2024 Feb 28
TI  - Expression of Major Histocompatibility Complex during Neuronal Differentiation of 
      Somatic Cell Nuclear Transfer-Human Embryonic Stem Cells.
PG  - 59-69
LID - 10.15283/ijsc23037 [doi]
AB  - Human pluripotent stem cells (hPSCs) such as human embryonic stem cells (hESCs), 
      induced pluripotent stem cells, and somatic cell nuclear transfer (SCNT)-hESCs 
      can permanently self-renew while maintaining their capacity to differentiate into 
      any type of somatic cells, thereby serving as an important cell source for cell 
      therapy. However, there are persistent challenges in the application of hPSCs in 
      clinical trials, where one of the most significant is graft rejection by the 
      patient immune system in response to human leukocyte antigen (HLA) mismatch when 
      transplants are obtained from an allogeneic (non-self) cell source. Homozygous 
      SCNT-hESCs (homo-SCNT-hESCs) were used to simplify the clinical application and 
      to reduce HLA mismatch. Here, we present a xeno-free protocol that confirms the 
      efficient generation of neural precursor cells in hPSCs and also the 
      differentiation of dopaminergic neurons. Additionally, there was no difference 
      when comparing the HLA expression patterns of hESC, homo-SCNT-hESCs and 
      hetero-SCNT-hESCs. We propose that there are no differences in the 
      differentiation capacity and HLA expression among hPSCs that can be cultured in 
      vitro. Thus, it is expected that homo-SCNT-hESCs will possess a wider range of 
      applications when transplanted with neural precursor cells in the context of 
      clinical trials.
FAU - Lee, Jin Saem
AU  - Lee JS
AUID- ORCID: 0000-0002-3718-227X
AD  - Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 
      Korea.
FAU - Lee, Jeoung Eun
AU  - Lee JE
AUID- ORCID: 0009-0009-7722-6909
AD  - CHA Advanced Research Institute, CHA University, Seongnam, Korea.
FAU - Yu, Shin-Hye
AU  - Yu SH
AUID- ORCID: 0000-0003-1810-9269
AD  - Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 
      Korea.
AD  - Paeanbiotechnology, Seoul, Korea.
FAU - Chun, Taehoon
AU  - Chun T
AUID- ORCID: 0000-0002-5940-8620
AD  - Division of Biotechnology, College of Life Sciences and Biotechnology, Korea 
      University, Seoul, Korea.
FAU - Chang, Mi-Yoon
AU  - Chang MY
AUID- ORCID: 0000-0003-4176-5520
AD  - Department of Premedicine, College of Medicine, Hanyang University, Seoul, Korea.
AD  - Hanyang Institute of Bioscience and Biotechnology, Hanyang University, Seoul, 
      Korea.
AD  - Hanyang Biomedical Research Institute, Hanyang University, Seoul, Korea.
FAU - Lee, Dong Ryul
AU  - Lee DR
AUID- ORCID: 0000-0003-1534-4810
AD  - CHA Advanced Research Institute, CHA University, Seongnam, Korea.
AD  - Department of Biomedical Science, College of Life Science, CHA University, 
      Seongnam, Korea.
FAU - Park, Chang-Hwan
AU  - Park CH
AUID- ORCID: 0000-0003-3510-9295
AD  - Graduate School of Biomedical Science and Engineering, Hanyang University, Seoul, 
      Korea.
AD  - Hanyang Biomedical Research Institute, Hanyang University, Seoul, Korea.
LA  - eng
PT  - Journal Article
DEP - 20231026
PL  - Korea (South)
TA  - Int J Stem Cells
JT  - International journal of stem cells
JID - 101497587
PMC - PMC10899886
OTO - NOTNLM
OT  - Dopaminergic differentiation
OT  - HLA expression
OT  - Human SCNT ESC
OT  - Neuronal differentiation
COIS- Potential Conflict of Interest There is no potential conflict of interest to 
      declare.
EDAT- 2023/10/26 00:42
MHDA- 2023/10/26 00:43
PMCR- 2023/10/26
CRDT- 2023/10/25 21:12
PHST- 2023/03/27 00:00 [received]
PHST- 2023/08/31 00:00 [revised]
PHST- 2023/09/08 00:00 [accepted]
PHST- 2023/10/26 00:43 [medline]
PHST- 2023/10/26 00:42 [pubmed]
PHST- 2023/10/25 21:12 [entrez]
PHST- 2023/10/26 00:00 [pmc-release]
AID - ijsc23037 [pii]
AID - ijsc-17-1-59 [pii]
AID - 10.15283/ijsc23037 [doi]
PST - ppublish
SO  - Int J Stem Cells. 2024 Feb 28;17(1):59-69. doi: 10.15283/ijsc23037. Epub 2023 Oct 
      26.