PMID- 37883506
OWN - NLM
STAT- MEDLINE
DCOM- 20231030
LR  - 20231101
IS  - 1932-6203 (Electronic)
IS  - 1932-6203 (Linking)
VI  - 18
IP  - 10
DP  - 2023
TI  - Hepatic metabolism of grazing cows of two Holstein strains under two feeding 
      strategies with different levels of pasture inclusion.
PG  - e0290551
LID - 10.1371/journal.pone.0290551 [doi]
LID - e0290551
AB  - The objective of the study was to characterize adaptations of hepatic metabolism 
      of dairy cows of two Holstein strains with varying proportions of grazing in the 
      feeding strategy. Multiparous autumn calving Holstein cows of New Zealand (NZH) 
      and North American (NAH) strains were assigned to a randomized complete block 
      design with a 2 x 2 factorial arrangement with two feeding strategies that varied 
      in the proportions of pasture and supplementation: maximum pasture and 
      supplementation with a pelleted concentrate (MaxP) or fixed pasture and 
      supplementation with a total mixed ration (FixP) from May through November of 
      2018. Hepatic biopsies were taken at - 45 +/- 17, 21 +/- 7, 100 +/- 23 and 180 +/- 23 
      days in milk (DIM), representing prepartum, early lactation, early mid-lactation 
      and late mid-lactation. The effects of DIM, feeding strategy (FS), strain and 
      their interactions were analyzed with mixed models using repeated measures. Cows 
      of both strains had similar triglyceride levels, mitochondrial function and 
      carnitine palmitoyltransferase activity in liver during lactation. However, there 
      was an effect of DIM and FS as liver triglyceride was higher for the MaxP 
      strategy at 21 DIM and both mitochondrial function and carnitine 
      palmitoyltransferase activity in liver were lower for the MaxP strategy at 21 
      DIM. Hepatic mitochondrial function and acetylation levels were affected by the 
      interaction between strain and feeding strategy as both variables were higher for 
      NAH cows in the MaxP strategy. Mid-lactation hepatic gene expression of enzymes 
      related to fatty acid metabolism and nuclear receptors was higher for NZH than 
      NAH cows. This work confirms the association between liver triglyceride, 
      decreased hepatic mitochondrial function and greater mitochondrial acetylation 
      levels in cows with a higher inclusion of pasture and suggests differential 
      adaptative mechanisms between NAH and NZH cows to strategies with varying 
      proportions of grazing in the feeding strategy.
CI  - Copyright: (c) 2023 Garcia-Roche et al. This is an open access article distributed 
      under the terms of the Creative Commons Attribution License, which permits 
      unrestricted use, distribution, and reproduction in any medium, provided the 
      original author and source are credited.
FAU - Garcia-Roche, Mercedes
AU  - Garcia-Roche M
AUID- ORCID: 0000-0002-2759-8829
AD  - Facultad de Agronomia, Departamento de Produccion Animal y Pasturas, Universidad 
      de la Republica, Montevideo, Uruguay.
AD  - Facultad de Medicina, Centro de Investigaciones Biomedicas (CEINBIO) and 
      Departamento de Bioquimica, Universidad de la Republica, Montevideo, Uruguay.
FAU - Talmon, Daniel
AU  - Talmon D
AD  - Facultad de Agronomia, Departamento de Produccion Animal y Pasturas, Universidad 
      de la Republica, Montevideo, Uruguay.
FAU - Canibe, Guillermo
AU  - Canibe G
AD  - Facultad de Agronomia, Departamento de Produccion Animal y Pasturas, Universidad 
      de la Republica, Montevideo, Uruguay.
FAU - Astessiano, Ana Laura
AU  - Astessiano AL
AD  - Facultad de Agronomia, Departamento de Produccion Animal y Pasturas, Universidad 
      de la Republica, Montevideo, Uruguay.
FAU - Mendoza, Alejandro
AU  - Mendoza A
AD  - Instituto Nacional de Investigacion Agropecuaria, Programa Nacional de Produccion 
      de Leche, Ruta, Semillero, Uruguay.
FAU - Cassina, Adriana
AU  - Cassina A
AD  - Facultad de Medicina, Centro de Investigaciones Biomedicas (CEINBIO) and 
      Departamento de Bioquimica, Universidad de la Republica, Montevideo, Uruguay.
FAU - Quijano, Celia
AU  - Quijano C
AUID- ORCID: 0000-0001-9254-7123
AD  - Facultad de Medicina, Centro de Investigaciones Biomedicas (CEINBIO) and 
      Departamento de Bioquimica, Universidad de la Republica, Montevideo, Uruguay.
FAU - Carriquiry, Mariana
AU  - Carriquiry M
AD  - Facultad de Agronomia, Departamento de Produccion Animal y Pasturas, Universidad 
      de la Republica, Montevideo, Uruguay.
LA  - eng
PT  - Journal Article
PT  - Randomized Controlled Trial, Veterinary
PT  - Research Support, Non-U.S. Gov't
DEP - 20231026
PL  - United States
TA  - PLoS One
JT  - PloS one
JID - 101285081
RN  - EC 2.3.1.21 (Carnitine O-Palmitoyltransferase)
RN  - 0 (Triglycerides)
SB  - IM
MH  - Female
MH  - Cattle
MH  - Animals
MH  - *Diet/veterinary
MH  - *Dietary Supplements
MH  - Carnitine O-Palmitoyltransferase/metabolism
MH  - Lactation/physiology
MH  - Milk/metabolism
MH  - Triglycerides/metabolism
PMC - PMC10602316
COIS- The authors have declared that no competing interests exist.
EDAT- 2023/10/26 18:42
MHDA- 2023/10/30 06:46
PMCR- 2023/10/26
CRDT- 2023/10/26 13:55
PHST- 2023/03/22 00:00 [received]
PHST- 2023/08/09 00:00 [accepted]
PHST- 2023/10/30 06:46 [medline]
PHST- 2023/10/26 18:42 [pubmed]
PHST- 2023/10/26 13:55 [entrez]
PHST- 2023/10/26 00:00 [pmc-release]
AID - PONE-D-23-08619 [pii]
AID - 10.1371/journal.pone.0290551 [doi]
PST - epublish
SO  - PLoS One. 2023 Oct 26;18(10):e0290551. doi: 10.1371/journal.pone.0290551. 
      eCollection 2023.