PMID- 37888561
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231029
IS  - 2306-7381 (Electronic)
IS  - 2306-7381 (Linking)
VI  - 10
IP  - 10
DP  - 2023 Oct 7
TI  - Emergent Molecular Techniques Applied to the Detection of Porcine Viruses.
LID - 10.3390/vetsci10100609 [doi]
LID - 609
AB  - Molecular diagnostic tests have evolved very rapidly in the field of human 
      health, especially with the arrival of the recent pandemic caused by the 
      SARS-CoV-2 virus. However, the animal sector is constantly neglected, even though 
      accurate detection by molecular tools could represent economic advantages by 
      preventing the spread of viruses. In this regard, the swine industry is of great 
      interest. The main viruses that affect the swine industry are described in this 
      review, including African swine fever virus (ASFV), porcine reproductive and 
      respiratory syndrome virus (PRRSV), porcine epidemic diarrhea virus (PEDV), and 
      porcine circovirus (PCV), which have been effectively detected by different 
      molecular tools in recent times. Here, we describe the rationale of molecular 
      techniques such as multiplex PCR, isothermal methods (LAMP, NASBA, RPA, and PSR) 
      and novel methods such as CRISPR-Cas and microfluidics platforms. Successful 
      molecular diagnostic developments are presented by highlighting their most 
      important findings. Finally, we describe the barriers that hinder the large-scale 
      development of affordable, accessible, rapid, and easy-to-use molecular 
      diagnostic tests. The evolution of diagnostic techniques is critical to prevent 
      the spread of viruses and the development of viral reservoirs in the swine 
      industry that impact the possible development of future pandemics and the world 
      economy.
FAU - Flores-Contreras, Elda A
AU  - Flores-Contreras EA
AUID- ORCID: 0000-0003-1046-6480
AD  - Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, 
      Nuevo Leon, Mexico.
AD  - Institute of Advanced Materials for Sustainable Manufacturing, Tecnologico de 
      Monterrey, Monterrey 64849, Nuevo Leon, Mexico.
FAU - Carrasco-Gonzalez, Jorge Alberto
AU  - Carrasco-Gonzalez JA
AD  - Biosafety, Lomas de los Pinos No. 5505-F, Col. La Estanzuela vieja, Monterrey 
      64984, Nuevo Leon, Mexico.
FAU - Linhares, Daniel C L
AU  - Linhares DCL
AUID- ORCID: 0000-0001-7788-9942
AD  - Veterinary Diagnostic and Production Animal Medicine Department, College of 
      Veterinary Medicine, Iowa State University, Ames, IA 50011, USA.
FAU - Corzo, Cesar A
AU  - Corzo CA
AUID- ORCID: 0000-0002-2749-3496
AD  - Veterinary Population Medicine Department, College of Veterinary Medicine, 
      University of Minnesota, St. Paul, MN 55455, USA.
FAU - Campos-Villalobos, J Israel
AU  - Campos-Villalobos JI
AD  - Proteina Animal S.A. de C.V. (PROAN), San Juan de los Lagos 47000, Jalisco, 
      Mexico.
FAU - Henao-Diaz, Alexandra
AU  - Henao-Diaz A
AD  - Industrias Bachoco, S.A. de C.V., Celaya, Celaya 38010, Guanajuato, Mexico.
FAU - Melchor-Martinez, Elda M
AU  - Melchor-Martinez EM
AUID- ORCID: 0000-0002-5434-2765
AD  - Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, 
      Nuevo Leon, Mexico.
AD  - Institute of Advanced Materials for Sustainable Manufacturing, Tecnologico de 
      Monterrey, Monterrey 64849, Nuevo Leon, Mexico.
FAU - Iqbal, Hafiz M N
AU  - Iqbal HMN
AUID- ORCID: 0000-0003-4855-2720
AD  - Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, 
      Nuevo Leon, Mexico.
AD  - Institute of Advanced Materials for Sustainable Manufacturing, Tecnologico de 
      Monterrey, Monterrey 64849, Nuevo Leon, Mexico.
FAU - Gonzalez-Gonzalez, Reyna Berenice
AU  - Gonzalez-Gonzalez RB
AUID- ORCID: 0000-0002-3022-7746
AD  - Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, 
      Nuevo Leon, Mexico.
AD  - Institute of Advanced Materials for Sustainable Manufacturing, Tecnologico de 
      Monterrey, Monterrey 64849, Nuevo Leon, Mexico.
FAU - Parra-Saldivar, Roberto
AU  - Parra-Saldivar R
AUID- ORCID: 0000-0002-4958-5797
AD  - Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, 
      Nuevo Leon, Mexico.
AD  - Institute of Advanced Materials for Sustainable Manufacturing, Tecnologico de 
      Monterrey, Monterrey 64849, Nuevo Leon, Mexico.
FAU - Gonzalez-Gonzalez, Everardo
AU  - Gonzalez-Gonzalez E
AUID- ORCID: 0000-0001-6588-5856
AD  - Tecnologico de Monterrey, School of Engineering and Sciences, Monterrey 64849, 
      Nuevo Leon, Mexico.
LA  - eng
PT  - Journal Article
PT  - Review
DEP - 20231007
PL  - Switzerland
TA  - Vet Sci
JT  - Veterinary sciences
JID - 101680127
PMC - PMC10610968
OTO - NOTNLM
OT  - ASFV
OT  - LAMP
OT  - NASBA
OT  - PCR
OT  - PCV
OT  - PEDV
OT  - PRRSV
OT  - PSR
OT  - RPA
COIS- The authors declare no conflict of interest.
EDAT- 2023/10/27 12:43
MHDA- 2023/10/27 12:44
PMCR- 2023/10/07
CRDT- 2023/10/27 07:20
PHST- 2023/07/28 00:00 [received]
PHST- 2023/09/16 00:00 [revised]
PHST- 2023/09/17 00:00 [accepted]
PHST- 2023/10/27 12:44 [medline]
PHST- 2023/10/27 12:43 [pubmed]
PHST- 2023/10/27 07:20 [entrez]
PHST- 2023/10/07 00:00 [pmc-release]
AID - vetsci10100609 [pii]
AID - vetsci-10-00609 [pii]
AID - 10.3390/vetsci10100609 [doi]
PST - epublish
SO  - Vet Sci. 2023 Oct 7;10(10):609. doi: 10.3390/vetsci10100609.