PMID- 37889059
OWN - NLM
STAT- MEDLINE
DCOM- 20231102
LR  - 20240422
IS  - 1790-6245 (Electronic)
IS  - 1109-6535 (Print)
IS  - 1109-6535 (Linking)
VI  - 20
IP  - 6
DP  - 2023 Nov-Dec
TI  - Hsa_circ_0079557 Promotes the Proliferation of Colorectal Cancer Cells Through 
      the hsa_circ_0079557/miR-502-5p/CCND1 Axis.
PG  - 567-581
LID - 10.21873/cgp.20406 [doi]
AB  - BACKGROUND/AIM: Recent studies have demonstrated the crucial regulatory roles of 
      circular RNAs (circRNAs) in cancer initiation and progression. The sponge 
      mechanism of circRNAs has been shown to be widely active in various types of 
      tumors. However, many circRNAs still have not been verified to function through 
      this mechanism. This study aimed to investigate the regulatory mechanism of 
      hsa_circ_0079557 in colorectal cancer (CRC) and its role in CRC progression. 
      MATERIALS AND METHODS: Raw gene expression profile datasets were downloaded from 
      Gene Expression Omnibus (GEO) and combined to form a new dataset. 
      Hsa_circ_0079557 was found to be highly expressed in CRC. Its role was evaluated 
      in vitro and in vivo through a series of experiments, including quantitative 
      real-time polymerase chain reaction (qRT-PCR), flow cytometry, colony formation, 
      cell counting kit-8 (CCK-8), transwell assays, scratch wound healing assays, nude 
      mice experiments, and immunohistochemistry (IHC). The association between 
      hsa_circ_0079557 and the identified target microRNAs (miRNA) was confirmed 
      through fluorescence in situ hybridization (FISH) and dual-luciferase reporter 
      assays. The downstream target proteins were predicted using the web-based tool 
      "TargetScan," and their expressions were determined using Western blot (WB). 
      RESULTS: Hsa_circ_0079557 was found to be relatively up-regulated in CRC tissues 
      and cell lines. Suppression of hsa_circ_0079557 expression inhibited cell 
      proliferation in vitro and in vivo. Additionally, hsa_circ_0079557 acted as a 
      "molecular sponge" for miR-502-5p, up-regulating the expression of Cyclin D1 
      (CCND1). CONCLUSION: In this study, we identify a highly expressed circRNA in CRC 
      and propose a novel pathway of hsa_circ_0079557/miR-502-5p/CCND1 in CRC.
CI  - Copyright (c) 2023, International Institute of Anticancer Research (Dr. George J. 
      Delinasios), All rights reserved.
FAU - Yu, Chao
AU  - Yu C
AD  - Department of General Surgery, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, P.R. China.
FAU - Huang, Xue
AU  - Huang X
AD  - Department of General Surgery, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, P.R. China.
FAU - Huang, Renli
AU  - Huang R
AD  - Department of General Surgery, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, P.R. China.
FAU - Wang, Peiqi
AU  - Wang P
AD  - Department of General Surgery, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, P.R. China.
FAU - Cai, Zongda
AU  - Cai Z
AD  - Department of Gastrointestinal Sugery, First Quanzhou Hospital, Fujian Medical 
      University, Quanzhou, P.R. China.
FAU - Guo, Zeyi
AU  - Guo Z
AD  - Department of Hepatobiliary Surgery, Zhujiang Hospital, Southern Medical 
      University, Guangzhou, P.R. China.
FAU - Lan, Qingnan
AU  - Lan Q
AD  - Department of General Surgery, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, P.R. China.
FAU - Cao, Haodi
AU  - Cao H
AD  - Department of Endocrinology, Zhujiang Hospital, Southern Medical University, 
      Guangdong, P.R. China.
FAU - Yu, Jinlong
AU  - Yu J
AD  - Department of General Surgery, Zhujiang Hospital, Southern Medical University, 
      Guangzhou, P.R. China; yujinlong640506@163.com.
LA  - eng
PT  - Journal Article
PL  - Greece
TA  - Cancer Genomics Proteomics
JT  - Cancer genomics & proteomics
JID - 101188791
RN  - 0 (CCND1 protein, human)
RN  - 136601-57-5 (Cyclin D1)
RN  - 0 (MicroRNAs)
RN  - 0 (MIRN502 microRNA, human)
RN  - 0 (RNA, Circular)
SB  - IM
MH  - Animals
MH  - Humans
MH  - Mice
MH  - Cell Proliferation/genetics
MH  - *Colorectal Neoplasms/genetics/metabolism
MH  - Cyclin D1/genetics/metabolism
MH  - In Situ Hybridization, Fluorescence
MH  - Mice, Nude
MH  - *MicroRNAs/genetics/metabolism
MH  - *RNA, Circular/genetics/metabolism
PMC - PMC10614065
OTO - NOTNLM
OT  - CCND1
OT  - CRC
OT  - Colorectal cancer
OT  - dataset
OT  - hsa_circ_0079557
OT  - miR-502-5p
COIS- The Authors declare that they have no conflicts of interest.
EDAT- 2023/10/27 12:42
MHDA- 2023/10/27 12:43
PMCR- 2023/11/03
CRDT- 2023/10/27 09:14
PHST- 2023/06/02 00:00 [received]
PHST- 2023/07/22 00:00 [revised]
PHST- 2023/08/04 00:00 [accepted]
PHST- 2023/10/27 12:43 [medline]
PHST- 2023/10/27 12:42 [pubmed]
PHST- 2023/10/27 09:14 [entrez]
PHST- 2023/11/03 00:00 [pmc-release]
AID - 20/6/567 [pii]
AID - 10.21873/cgp.20406 [doi]
PST - ppublish
SO  - Cancer Genomics Proteomics. 2023 Nov-Dec;20(6):567-581. doi: 10.21873/cgp.20406.