PMID- 37889278
OWN - NLM
STAT- MEDLINE
DCOM- 20240109
LR  - 20240109
IS  - 1432-2072 (Electronic)
IS  - 0033-3158 (Linking)
VI  - 241
IP  - 1
DP  - 2024 Jan
TI  - Improving behavioral deficits induced by perinatal ethanol and stress exposure in 
      adolescent male rat progeny via maternal melatonin treatment.
PG  - 153-169
LID - 10.1007/s00213-023-06470-z [doi]
AB  - BACKGROUND AND AIM: Early-life stressful situations and binge drinking have been 
      thus far acknowledged as two burdensome conditions that potentially give rise to 
      negative outcomes and then synergistically affect brain development. In this 
      context, the hippocampus, with the greatest number of glucocorticoid receptors 
      (GCRs) in the brain, is responsible for regulating negative responses to stress. 
      Prolonged glucocorticoid (GC) exposure can accordingly cause oxidative stress 
      (OS), leading to cognitive and emotional dysfunction. Against this background, 
      melatonin, as a powerful antioxidant and hypothalamus-pituitary-adrenal (HPA) 
      axis regulator, was administered in this study to ameliorate cognitive 
      impairments induced by perinatal ethanol and stress exposure in adolescent male 
      rat progeny. METHODS: Wistar rat dams were exposed to ethanol (4 g/kg) and 
      melatonin (10 mg/kg) from gestational day (GD) 6 to postnatal day (PND) 14 and 
      then limited nesting material (LNS) from PND0 to PND14 individually or in 
      combination. Maternal behavior was then investigated in mothers. Afterward, the 
      plasma corticosterone (CORT) concentration, the OS marker, the 
      corticotropin-releasing hormone receptor type 1 (CRHR1) expression, and the GCR 
      and brain-derived neurotrophic factor (BDNF) levels were measured in the male 
      pups. Moreover, behavioral tasks, including the elevated plus maze (EPM), the 
      Morris water maze (MWM), the novel object recognition (NORT), and the 
      object-location memory (OLM) tests were completed and assessed. RESULTS: The 
      quantity and quality of maternal care significantly decreased in the mothers with 
      dual exposure to ethanol and stress. The plasma CORT concentration in the progeny 
      also dropped in the Ethanol + LNS group, but the risk-taking behavior elevated 
      significantly. The ethanol and stress exposure further revealed a significant 
      fall in the GCR and CRHR1 expression levels, compared with stress alone. The 
      results of learning and memory tasks also indicated a significant reduction in 
      spatial learning and memory among animals exposed to ethanol and stress. The BDNF 
      mRNA levels correspondingly increased in the Ethanol + LNS group, compared with 
      LNS alone. In the presence of ethanol and stress, the superoxide dismutase (SOD) 
      and glutathione peroxidase (GPx) activities correspondingly declined. On the 
      other hand, the malondialdehyde (MDA) levels augmented in the hippocampus of the 
      animals with ethanol and LNS dual exposure, as compared with the control group. 
      Melatonin treatment (MT) thus improved nursing behaviors in dams, prevented OS, 
      enhanced the CRHR1 and GCR expression, and reduced the BDNF levels to the similar 
      ones in the control group. The animals in the Ethanol + LNS + MT group ultimately 
      showed an ameliorated performance at behavioral tasks, including the memory and 
      risk-taking behavior. CONCLUSION: It was concluded that MT could prevent stress 
      response and memory impairments arising from dual exposure to ethanol and stress 
      by inhibiting OS.
CI  - (c) 2023. The Author(s), under exclusive licence to Springer-Verlag GmbH Germany, 
      part of Springer Nature.
FAU - Bagheri, Farzaneh
AU  - Bagheri F
AD  - School of Biology, Damghan University, Damghan, Iran.
FAU - Goudarzi, Iran
AU  - Goudarzi I
AD  - School of Biology, Damghan University, Damghan, Iran. irangoudarzi@du.ac.ir.
FAU - Lashkarbolouki, Taghi
AU  - Lashkarbolouki T
AD  - School of Biology, Damghan University, Damghan, Iran.
FAU - Elahdadi Salmani, Mahmoud
AU  - Elahdadi Salmani M
AD  - School of Biology, Damghan University, Damghan, Iran.
FAU - Goudarzi, Afsaneh
AU  - Goudarzi A
AD  - Department of Clinical Biochemistry, School of Medicine, Shahid Beheshti 
      University of Medical Sciences, Tehran, Iran.
FAU - Morley-Fletcher, Sara
AU  - Morley-Fletcher S
AD  - Univ. Lille, CNRS, UMR 8576 - UGSF - Unite de Glycobiologie Structurale Et 
      Fonctionnelle, 59000, Lille, France.
LA  - eng
PT  - Journal Article
DEP - 20231027
PL  - Germany
TA  - Psychopharmacology (Berl)
JT  - Psychopharmacology
JID - 7608025
RN  - JL5DK93RCL (Melatonin)
RN  - 3K9958V90M (Ethanol)
RN  - 0 (Brain-Derived Neurotrophic Factor)
RN  - 0 (Antioxidants)
SB  - IM
MH  - Pregnancy
MH  - Female
MH  - Rats
MH  - Animals
MH  - Male
MH  - *Melatonin/pharmacology/metabolism
MH  - Rats, Wistar
MH  - Ethanol/toxicity
MH  - Brain-Derived Neurotrophic Factor/metabolism
MH  - Antioxidants/metabolism
MH  - Spatial Learning
MH  - Hippocampus/metabolism
MH  - Maze Learning
OTO - NOTNLM
OT  - Early-life stress
OT  - Ethanol
OT  - Learning and memory
OT  - Melatonin
OT  - Oxidative stress
OT  - Rat pups
OT  - Risk-taking behavior
EDAT- 2023/10/27 12:42
MHDA- 2024/01/09 06:42
CRDT- 2023/10/27 11:04
PHST- 2023/02/10 00:00 [received]
PHST- 2023/09/25 00:00 [accepted]
PHST- 2024/01/09 06:42 [medline]
PHST- 2023/10/27 12:42 [pubmed]
PHST- 2023/10/27 11:04 [entrez]
AID - 10.1007/s00213-023-06470-z [pii]
AID - 10.1007/s00213-023-06470-z [doi]
PST - ppublish
SO  - Psychopharmacology (Berl). 2024 Jan;241(1):153-169. doi: 
      10.1007/s00213-023-06470-z. Epub 2023 Oct 27.