PMID- 37889294
OWN - NLM
STAT- MEDLINE
DCOM- 20231030
LR  - 20231030
IS  - 1540-8140 (Electronic)
IS  - 0021-9525 (Print)
IS  - 0021-9525 (Linking)
VI  - 223
IP  - 1
DP  - 2024 Jan 1
TI  - Vinculin is required for interkinetic nuclear migration (INM) and cell cycle 
      progression.
LID - 10.1083/jcb.202106169 [doi]
LID - e202106169
AB  - Vinculin is an actin-binding protein (ABP) that strengthens the connection 
      between the actin cytoskeleton and adhesion complexes. It binds to 
      beta-catenin/N-cadherin complexes in apical adherens junctions (AJs), which maintain 
      cell-to-cell adhesions, and to talin/integrins in the focal adhesions (FAs) that 
      attach cells to the basal membrane. Here, we demonstrate that beta-catenin targets 
      vinculin to the apical AJs and the centrosome in the embryonic neural tube (NT). 
      Suppression of vinculin slows down the basal-to-apical part of interkinetic 
      nuclear migration (BAINM), arrests neural stem cells (NSCs) in the G2 phase of 
      the cell cycle, and ultimately dismantles the apical actin cytoskeleton. In the 
      NSCs, mitosis initiates when an internalized centrosome gathers with the nucleus 
      during BAINM. Notably, our results show that the first centrosome to be 
      internalized is the daughter centrosome, where beta-catenin and vinculin accumulate, 
      and that vinculin suppression prevents centrosome internalization. Thus, we 
      propose that vinculin links AJs, the centrosome, and the actin cytoskeleton where 
      actomyosin contraction forces are required.
CI  - (c) 2023 Ochoa et al.
FAU - Ochoa, Andrea
AU  - Ochoa A
AUID- ORCID: 0009-0005-1454-6025
AD  - Instituto de Biologia Molecular de Barcelona (CSIC) , Barcelona, Spain.
FAU - Herrera, Antonio
AU  - Herrera A
AUID- ORCID: 0000-0002-6248-1001
AD  - Instituto de Biologia Molecular de Barcelona (CSIC) , Barcelona, Spain.
FAU - Menendez, Anghara
AU  - Menendez A
AUID- ORCID: 0000-0001-6513-0584
AD  - Instituto de Biologia Molecular de Barcelona (CSIC) , Barcelona, Spain.
FAU - Estefanell, Maria
AU  - Estefanell M
AUID- ORCID: 0009-0001-1869-7614
AD  - Instituto de Biologia Molecular de Barcelona (CSIC) , Barcelona, Spain.
FAU - Ramos, Carlota
AU  - Ramos C
AUID- ORCID: 0009-0009-5238-9891
AD  - Instituto de Biologia Molecular de Barcelona (CSIC) , Barcelona, Spain.
FAU - Pons, Sebastian
AU  - Pons S
AUID- ORCID: 0000-0003-1027-0621
AD  - Instituto de Biologia Molecular de Barcelona (CSIC) , Barcelona, Spain.
LA  - eng
PT  - Journal Article
PT  - Research Support, Non-U.S. Gov't
DEP - 20231027
PL  - United States
TA  - J Cell Biol
JT  - The Journal of cell biology
JID - 0375356
RN  - 0 (Actins)
RN  - 0 (beta Catenin)
RN  - 125361-02-6 (Vinculin)
SB  - IM
MH  - Actin Cytoskeleton/metabolism
MH  - Actins/metabolism
MH  - beta Catenin/genetics/metabolism
MH  - Cell Adhesion
MH  - *Cell Cycle
MH  - *Focal Adhesions/genetics/metabolism
MH  - *Vinculin/genetics
MH  - Neural Stem Cells/cytology
MH  - Centrosome/metabolism
MH  - Adherens Junctions/metabolism
PMC - PMC10609122
COIS- Disclosures: The authors declare no competing interests exist.
EDAT- 2023/10/27 12:44
MHDA- 2023/10/30 06:46
PMCR- 2023/10/27
CRDT- 2023/10/27 11:04
PHST- 2021/06/28 00:00 [received]
PHST- 2023/08/08 00:00 [revised]
PHST- 2023/10/12 00:00 [accepted]
PHST- 2023/10/30 06:46 [medline]
PHST- 2023/10/27 12:44 [pubmed]
PHST- 2023/10/27 11:04 [entrez]
PHST- 2023/10/27 00:00 [pmc-release]
AID - 276364 [pii]
AID - jcb.202106169 [pii]
AID - 10.1083/jcb.202106169 [doi]
PST - ppublish
SO  - J Cell Biol. 2024 Jan 1;223(1):e202106169. doi: 10.1083/jcb.202106169. Epub 2023 
      Oct 27.