PMID- 37889543
OWN - NLM
STAT- MEDLINE
DCOM- 20231107
LR  - 20231112
IS  - 1945-4589 (Electronic)
IS  - 1945-4589 (Linking)
VI  - 15
IP  - 20
DP  - 2023 Oct 26
TI  - Metabolic and senescence characteristics associated with the immune 
      microenvironment in non-small cell lung cancer: insights from single-cell RNA 
      sequencing.
PG  - 11571-11587
LID - 10.18632/aging.205146 [doi]
AB  - Non-small lung cancer (NSCLC) has been defined as a highly life-threatening 
      heterogeneous disease, with high mortality and occurrence. Recent research has 
      indicated that tumor-infiltrating lymphocytes play a key determinant role in 
      cancer progression. Emerging single-cell RNA sequencing (also termed scRNA-seq) 
      has been extensively applied to depict the baseline landscape of the cell 
      composition and function phenotype in the tumor environment (TME). Herein, we 
      dissected the cell types in NSCLC samples (including tissue and blood) and 
      identified three types of cell marker genes including cancer cells, T cells, and 
      macrophages by integrating two NSCLC-associated scRNA-seq datasets in GEO. 
      Survival analysis indicated that 17 marker genes were related to tumor prognosis. 
      Function annotation was used to scrutinize the molecular mechanism of these 
      marker genes in different cells. Besides, we investigated the developmental 
      trajectory and T cell receptor repertoire diversity of tumor-infiltrating T 
      cells. Our analysis will help further understand the complexity of cell 
      components and the heterogeneity of TME in NSCLC.
FAU - Liao, Hongliang
AU  - Liao H
AD  - Department of Thoracic Surgery, The Yuebei People’s Hospital of Shaoguan, 
      Shaoguan, Guangdong 512025, China.
FAU - Wan, Zihao
AU  - Wan Z
AD  - College of Physical Education and Health, Guangzhou University of Chinese 
      Medicine, Guangzhou, Guangdong, China.
FAU - Liang, Yaqin
AU  - Liang Y
AD  - Department of Nursing Medical College, Shaoguan University, Shaoguan, Guangdong 
      512005, China.
FAU - Kang, Lin
AU  - Kang L
AD  - Department of Gynaecology and Obstetrics, The Qujiang District Maternal and Child 
      Health Care Hospital, Shaoguan, Guangdong, China.
FAU - Wan, Renping
AU  - Wan R
AD  - Department of Thoracic Surgery, The Yuebei People’s Hospital of Shaoguan, 
      Shaoguan, Guangdong 512025, China.
LA  - eng
PT  - Journal Article
DEP - 20231026
PL  - United States
TA  - Aging (Albany NY)
JT  - Aging
JID - 101508617
SB  - IM
MH  - Humans
MH  - *Carcinoma, Non-Small-Cell Lung/genetics
MH  - *Lung Neoplasms/genetics
MH  - Lymphocytes, Tumor-Infiltrating
MH  - Macrophages
MH  - Sequence Analysis, RNA
MH  - Tumor Microenvironment/genetics
PMC - PMC10637824
OTO - NOTNLM
OT  - NSCLC
OT  - T cell
OT  - TME
OT  - cell phenotype
OT  - immune cell
OT  - single cell
COIS- CONFLICTS OF INTEREST: The authors declare no conflicts of interest related to 
      this study.
EDAT- 2023/10/27 12:44
MHDA- 2023/11/07 06:45
PMCR- 2023/10/31
CRDT- 2023/10/27 11:54
PHST- 2023/06/12 00:00 [received]
PHST- 2023/10/06 00:00 [accepted]
PHST- 2023/11/07 06:45 [medline]
PHST- 2023/10/27 12:44 [pubmed]
PHST- 2023/10/27 11:54 [entrez]
PHST- 2023/10/31 00:00 [pmc-release]
AID - 205146 [pii]
AID - 10.18632/aging.205146 [doi]
PST - ppublish
SO  - Aging (Albany NY). 2023 Oct 26;15(20):11571-11587. doi: 10.18632/aging.205146. 
      Epub 2023 Oct 26.