PMID- 37890931
OWN - NLM
STAT- MEDLINE
DCOM- 20231030
LR  - 20231030
IS  - 0091-679X (Print)
IS  - 0091-679X (Linking)
VI  - 180
DP  - 2023
TI  - Methodologies to evaluate the radiation-induced changes on extracellular 
      vesicles.
PG  - 39-48
LID - S0091-679X(23)00064-X [pii]
LID - 10.1016/bs.mcb.2023.03.002 [doi]
AB  - The extracellular vesicles (EVs) are carriers that actively transfer functional 
      biomolecules between cells affecting the intercellular communication (Pitt, 
      Kroemer, & Zitvogel, 2016). EV signaling has consequences on the targeted cell 
      behavior impacting multiple processes from health to disease, including cancer 
      (Yates et al., 2022). Radiation treatment (RT) is one of the gold standard and 
      effective treatments for cancer, as curative or palliative (Chandra, Keane, 
      Voncken, & Thomas, 2021). RT induces different release of EVs and their cargo is 
      altered. In addition, the uptake of EVs secreted by irradiated cells is affected. 
      Hence, a deep investigation is required to better understand how RT influence the 
      cell-to-cell communication thought signals shuttle by EVs. Here, detailed methods 
      to study the RT effects on EV size and secretion, EV protein expression, EV 
      uptake will be described. Alterations and adaptions might make the protocols 
      applicable to different cell lines, and with different types of RT and dose 
      exposures.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Spada, Sheila
AU  - Spada S
AD  - Department of Radiation Oncology, Weill Cornell Medicine, New York, NY, United 
      States. Electronic address: sheilaspada88@gmail.com.
LA  - eng
PT  - Journal Article
DEP - 20230513
PL  - United States
TA  - Methods Cell Biol
JT  - Methods in cell biology
JID - 0373334
SB  - IM
MH  - Humans
MH  - *Extracellular Vesicles/metabolism
MH  - *Neoplasms/metabolism
MH  - Signal Transduction
MH  - Cell Communication
MH  - Proteomics
OTO - NOTNLM
OT  - Cancer
OT  - Cargo
OT  - Exosomes
OT  - Immunofluorescence
OT  - Ionizing radiation
OT  - Nanoparticles
OT  - Proteomics
OT  - Uptake
OT  - Western blot
EDAT- 2023/10/28 11:42
MHDA- 2023/10/30 06:46
CRDT- 2023/10/27 20:57
PHST- 2023/10/30 06:46 [medline]
PHST- 2023/10/28 11:42 [pubmed]
PHST- 2023/10/27 20:57 [entrez]
AID - S0091-679X(23)00064-X [pii]
AID - 10.1016/bs.mcb.2023.03.002 [doi]
PST - ppublish
SO  - Methods Cell Biol. 2023;180:39-48. doi: 10.1016/bs.mcb.2023.03.002. Epub 2023 May 
      13.