PMID- 37891121
OWN - NLM
STAT- Publisher
LR  - 20231027
IS  - 2212-4411 (Electronic)
DP  - 2023 Aug 1
TI  - Evaluation of EWSR1/FUS rearrangements by FISH and NKX2.2 immunoexpression in 
      simple bone cysts of the jaw.
LID - S2212-4403(23)00595-3 [pii]
LID - 10.1016/j.oooo.2023.07.050 [doi]
AB  - OBJECTIVE: Recent studies have identified recurrent rearrangements of EWSR1 or 
      FUS with NFATC2 in extragnathic simple bone cysts (SBCs). We investigated the 
      presence of EWSR1 or FUS rearrangements and the immunophenotypic expression of 
      NKX2.2 and CD99 in a series of SBCs of the jaw. STUDY DESIGN: We retrieved 10 
      cases of SBC of the jaw from the University of Pittsburgh archives. Of the 10 
      cases, we were able to evaluate 8 by immunohistochemistry for CD99 and NKX2.2 and 
      7 by fluorescence in situ hybridization (FISH) for EWSR1 and FUS rearrangement 
      using EWSR1 and FUS break-apart probes. RESULTS: All 8 cases evaluated by 
      immunohistochemistry expressed CD99 but were negative for NKX2.2, and all 7 cases 
      assayed using FISH were negative for EWSR1 and FUS rearrangements. CONCLUSIONS: 
      In contrast to the SBC of extragnathic sites, we found no presence of EWSR1 and 
      FUS rearrangements by FISH in the SBC of the jaw, suggesting that this entity may 
      be etiologically/molecularly distinct and reflects a non-neoplastic reactive 
      process. However, as these lesions tend to be paucicellular, FISH may not be the 
      appropriate technique for identifying EWSR1/FUS fusions. Other techniques should 
      be used to evaluate them in future studies.
CI  - Copyright (c) 2023 Elsevier Inc. All rights reserved.
FAU - Owosho, Adepitan A
AU  - Owosho AA
AD  - Department of Diagnostic Sciences, College of Dentistry, The University of 
      Tennessee Health Science Center, Memphis, TN, USA; Department of Otolaryngology, 
      College of Medicine, The University of Tennessee Health Science Center, Memphis, 
      TN, USA. Electronic address: Aowosho@uthsc.edu.
FAU - Shahrabi-Farahani, Shokoufeh
AU  - Shahrabi-Farahani S
AD  - Department of Diagnostic Sciences, College of Dentistry, The University of 
      Tennessee Health Science Center, Memphis, TN, USA.
FAU - DeColibus, Katherine A
AU  - DeColibus KA
AD  - Department of Diagnostic Sciences, College of Dentistry, The University of 
      Tennessee Health Science Center, Memphis, TN, USA.
FAU - Shasteen, Alivia M
AU  - Shasteen AM
AD  - Department of Diagnostic Sciences, School of Dental Medicine, University of 
      Pittsburgh, Pittsburgh, PA, USA.
FAU - Summersgill, Kurt F
AU  - Summersgill KF
AD  - Department of Diagnostic Sciences, School of Dental Medicine, University of 
      Pittsburgh, Pittsburgh, PA, USA.
LA  - eng
PT  - Journal Article
DEP - 20230801
PL  - United States
TA  - Oral Surg Oral Med Oral Pathol Oral Radiol
JT  - Oral surgery, oral medicine, oral pathology and oral radiology
JID - 101576782
SB  - IM
COIS- Declaration of interest None.
EDAT- 2023/10/28 11:42
MHDA- 2023/10/28 11:42
CRDT- 2023/10/27 22:58
PHST- 2023/04/29 00:00 [received]
PHST- 2023/06/08 00:00 [revised]
PHST- 2023/07/26 00:00 [accepted]
PHST- 2023/10/28 11:42 [medline]
PHST- 2023/10/28 11:42 [pubmed]
PHST- 2023/10/27 22:58 [entrez]
AID - S2212-4403(23)00595-3 [pii]
AID - 10.1016/j.oooo.2023.07.050 [doi]
PST - aheadofprint
SO  - Oral Surg Oral Med Oral Pathol Oral Radiol. 2023 Aug 1:S2212-4403(23)00595-3. 
      doi: 10.1016/j.oooo.2023.07.050.