PMID- 37891879
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231030
IS  - 2076-3921 (Print)
IS  - 2076-3921 (Electronic)
IS  - 2076-3921 (Linking)
VI  - 12
IP  - 10
DP  - 2023 Sep 25
TI  - DUOX2-Induced Oxidative Stress Inhibits Intestinal Angiogenesis through MMP3 in a 
      Low-Birth-Weight Piglet Model.
LID - 10.3390/antiox12101800 [doi]
LID - 1800
AB  - Intestinal vessels play a critical role in nutrient absorption, whereas the 
      effect and mechanism of low birth weight (LBW) on its formation remain unclear. 
      Here, twenty newborn piglets were assigned to the control (CON) group (1162 +/- 98 
      g) and LBW group (724 +/- 31 g) according to their birth weight. Results showed 
      that the villus height and the activity of maltase in the jejunum were lower in 
      the LBW group than in the CON group. LBW group exhibited a higher oxidative 
      stress level and impaired mitochondrial function in the jejunum and was lower 
      than the CON group in the intestinal vascular density. To investigate the role of 
      oxidative stress in intestinal angiogenesis, H(2)O(2) was employed to induce 
      oxidative stress in porcine intestinal epithelial cells (IPEC-J2). The results 
      showed that the conditioned media from IPEC-J2 with H(2)O(2) treatment decreased 
      the angiogenesis of porcine vascular endothelial cells (PVEC). Transcriptome 
      analysis revealed that a higher expression level of dual oxidase 2 (DUOX2) was 
      found in the intestine of LBW piglets. Knockdown of DUOX2 in IPEC-J2 increased 
      the proliferation and decreased the oxidative stress level. In addition, 
      conditioned media from IPEC-J2 with DUOX2-knockdown was demonstrated to promote 
      the angiogenesis of PVEC. Mechanistically, the knockdown of DUOX2 decreased the 
      reactive oxygen species (ROS) level, thus increasing the angiogenesis in a matrix 
      metalloproteinase 3 (MMP3) dependent manner. Conclusively, our results indicated 
      that DUOX2-induced oxidative stress inhibited intestinal angiogenesis through 
      MMP3 in a LBW piglet model.
FAU - Zou, Dongbin
AU  - Zou D
AD  - Tropical Crop Genetic Resource Research Institute, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
AD  - College of Life Sciences, Hainan University, Haikou 571101, China.
FAU - Yang, Yun
AU  - Yang Y
AD  - Tropical Crop Genetic Resource Research Institute, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
AD  - College of Animal Science and Technology, Huazhong Agricultural University, Wuhan 
      430070, China.
FAU - Ji, Fengjie
AU  - Ji F
AD  - Tropical Crop Genetic Resource Research Institute, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
FAU - Lv, Renlong
AU  - Lv R
AD  - Tropical Crop Genetic Resource Research Institute, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
FAU - Xu, Tieshan
AU  - Xu T
AD  - Tropical Crop Genetic Resource Research Institute, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
FAU - Hu, Chengjun
AU  - Hu C
AUID- ORCID: 0000-0001-6406-6445
AD  - Tropical Crop Genetic Resource Research Institute, Chinese Academy of Tropical 
      Agricultural Sciences, Haikou 571101, China.
LA  - eng
GR  - 322RC773 and 321QN320/Natural Science Foundation of Hainan Province/
GR  - 1630032023009/Central Public-interest Scientific Institution Basal Research Fund/
GR  - HNARS2022-2-G3/Hainan Modern Agricultural Production Research System/
PT  - Journal Article
DEP - 20230925
PL  - Switzerland
TA  - Antioxidants (Basel)
JT  - Antioxidants (Basel, Switzerland)
JID - 101668981
PMC - PMC10603984
OTO - NOTNLM
OT  - DUOX2
OT  - MMP3
OT  - intestinal angiogenesis
OT  - low birth weight
OT  - oxidative stress
COIS- The authors declare no conflict of interest.
EDAT- 2023/10/28 11:47
MHDA- 2023/10/28 11:48
PMCR- 2023/09/25
CRDT- 2023/10/28 01:01
PHST- 2023/06/27 00:00 [received]
PHST- 2023/09/13 00:00 [revised]
PHST- 2023/09/19 00:00 [accepted]
PHST- 2023/10/28 11:48 [medline]
PHST- 2023/10/28 11:47 [pubmed]
PHST- 2023/10/28 01:01 [entrez]
PHST- 2023/09/25 00:00 [pmc-release]
AID - antiox12101800 [pii]
AID - antioxidants-12-01800 [pii]
AID - 10.3390/antiox12101800 [doi]
PST - epublish
SO  - Antioxidants (Basel). 2023 Sep 25;12(10):1800. doi: 10.3390/antiox12101800.