PMID- 37894222
OWN - NLM
STAT- PubMed-not-MEDLINE
LR  - 20231030
IS  - 2076-2607 (Print)
IS  - 2076-2607 (Electronic)
IS  - 2076-2607 (Linking)
VI  - 11
IP  - 10
DP  - 2023 Oct 15
TI  - Differential Immune-Modulating Activities of Cell Walls and Secreted Metabolites 
      from Probiotic Bacillus coagulans JBI-YZ6.3 under Normal versus Inflamed Culture 
      Conditions.
LID - 10.3390/microorganisms11102564 [doi]
LID - 2564
AB  - Spore-forming probiotic bacteria, including Bacillus coagulans, are resilient and 
      produce a variety of beneficial metabolites. We evaluated the immune-modulating 
      effects of the novel probiotic strain Bacillus coagulans JBI-YZ6.3, where the 
      germinated spores, metabolite fraction, and cell wall fraction were tested in 
      parallel using human peripheral blood mononuclear cell cultures under both normal 
      and lipopolysaccharide-induced inflamed culture conditions. The expression of 
      CD25 and CD69 activation markers was evaluated via flow cytometry. Supernatants 
      were tested for cytokines, interferons, chemokines, and growth factors using 
      Luminex arrays. The germinated spores were highly immunogenic; both the cell wall 
      and metabolite fractions contributed significantly. Under normal culture 
      conditions, increased levels of immune activation were observed as increased 
      expressions of CD25 and CD69 relative to natural killer cells, suggesting an 
      increased ability to attack virus-infected target cells. On monocytes, a complex 
      effect was observed, where the expression of CD25 increased under normal 
      conditions but decreased under inflamed conditions. This, in combination with 
      increased interleukin-10 (IL-10) and decreased monocyte chemoattractant protein-1 
      (MCP-1) production under inflamed conditions, points to anti-inflammatory 
      effects. The production of the stem cell-related growth factor granulocyte 
      colony-stimulating Factor (G-CSF) was enhanced. Further research is warranted to 
      characterize the composition of the postbiotic metabolite fraction and document 
      the characteristics of immunomodulating agents secreted by this probiotic strain.
FAU - Iloba, Ifeanyi
AU  - Iloba I
AUID- ORCID: 0000-0001-9179-8369
AD  - NIS Labs, 1437 Esplanade, Klamath Falls, OR 97601, USA.
FAU - McGarry, Sage V
AU  - McGarry SV
AUID- ORCID: 0000-0003-4382-2605
AD  - NIS Labs, 807 St. George St., Port Dover, ON N0A 1N0, Canada.
FAU - Yu, Liu
AU  - Yu L
AUID- ORCID: 0000-0003-3204-2259
AD  - NIS Labs, 807 St. George St., Port Dover, ON N0A 1N0, Canada.
FAU - Cruickshank, Dina
AU  - Cruickshank D
AD  - NIS Labs, 807 St. George St., Port Dover, ON N0A 1N0, Canada.
FAU - Jensen, Gitte S
AU  - Jensen GS
AUID- ORCID: 0000-0002-9497-1750
AD  - NIS Labs, 1437 Esplanade, Klamath Falls, OR 97601, USA.
LA  - eng
GR  - na/Jeneil Biotech/
PT  - Journal Article
DEP - 20231015
PL  - Switzerland
TA  - Microorganisms
JT  - Microorganisms
JID - 101625893
PMC - PMC10609156
OTO - NOTNLM
OT  - T cells
OT  - anti-inflammatory
OT  - chemokines
OT  - cytokines
OT  - granulocyte colony-stimulating factor (G-CSF)
OT  - monocytes
OT  - natural killer (NK) cells
COIS- The authors declare no conflict of interest. The funders had no role in the 
      design of the study; in the collection, analyses, or interpretation of data; or 
      in the writing of the manuscript. The funders supported the decision to publish 
      the results.
EDAT- 2023/10/28 11:43
MHDA- 2023/10/28 11:44
PMCR- 2023/10/15
CRDT- 2023/10/28 01:14
PHST- 2023/09/16 00:00 [received]
PHST- 2023/10/06 00:00 [revised]
PHST- 2023/10/12 00:00 [accepted]
PHST- 2023/10/28 11:44 [medline]
PHST- 2023/10/28 11:43 [pubmed]
PHST- 2023/10/28 01:14 [entrez]
PHST- 2023/10/15 00:00 [pmc-release]
AID - microorganisms11102564 [pii]
AID - microorganisms-11-02564 [pii]
AID - 10.3390/microorganisms11102564 [doi]
PST - epublish
SO  - Microorganisms. 2023 Oct 15;11(10):2564. doi: 10.3390/microorganisms11102564.